Very low protein nutrient medium for cell culture

Claim 1

A nutrient medium for in vitro animal cell culture, consisting essentially of the following: ______________________________________ CaCl.sub.2.2H.sub.2 O 1 × 10.sup.-3 M CuSO.sub.4.5H.sub.2 O 3 × 10.sup.-9 M FeSO.sub.4.7H.sub.2 O 1 × 10.sup.-6 M Fe(NO.sub.3).sub.3.9H.sub.2 O 2 × 10.sup.-7 M KCl 4 × 10.sup.-3 M MgSO.sub.4.7H.sub.2 O 8 × 10.sup.-4 M NaCl 1.05 × 10.sup.-1 M Na.sub.2 HPO.sub.4.7H.sub.2 O 3 × 10.sup.-4 M NaH.sub.2 PO.sub.4.2H.sub.2 O 6 × 10.sup.-4 M Na.sub.2 SeO.sub.3.5H.sub.2 O 3 × 10.sup.-8 M Na.sub.2 SiO.sub.3.9H.sub.2 O 1 × 10.sup.-5 M (NH.sub.4)6Mo.sub.7 O.sub.24.4H.sub.2 O 3 × 10.sup.-9 M NH.sub.4 VO.sub.3 .sup. 5 × 10.sup.-10 M NiSO.sub.4.6H.sub.2 O .sup. 3 × 10.sup.-10 M ZnSO.sub.4.7H.sub.2 O 8 × 10.sup.-7 M inorganic iron source 5 × 10.sup.-5 M L-Arg 8 × 10.sup.-4 M L-Cys HCl.H.sub.2 O 3 × 10.sup.-4 M L-Gln 5 × 10.sup.-3 M L-His HCl.H.sub.2 O 2 × 10.sup.-4 M L-Hydroxy-Pro 1 × 10.sup.-4 M L-Ile 6 × 10.sup.-4 M L-Leu 6 × 10.sup.-4 M L-Lys HCl 8 × 10.sup.-4 M L-Met 1 × 10.sup.-3 M L-Phe 3 × 10.sup.-4 M L-Thr 6 × 10.sup.-4 M L-Trp 6 × 10.sup.-5 M L-Tyr (diNa.sup.+)2H.sub.2 O 3 × 10.sup.-4 M L-Val 6 × 10.sup.-4 M L-Ala 2 × 10.sup.-5 M L-Asn.H.sub.2 O 3 × 10.sup.-4 M L-Asp 2 × 10.sup.-5 M L-Glu 2 × 10.sup.-5 M Gly 3 × 10.sup.- 5 M L-Pro 2 × 10.sup.-4 M L-Ser 3 × 10.sup.-4 M Glutathione 1 × 10.sup.-6 M Putrescine 2HCl 3 × 10.sup.-7 M Biotin 3 × 10.sup.-8 M D-Ca pantothenate 2 × 10.sup.-5 M Folic acid 6 × 10.sup.-6 M Folinic acid (Ca.sup.+).5H.sub.2 O 1 × 10.sup.-6 M Niacinamide 3 × 10.sup.-5 M (Nicotinamide) p-Aminobenzoic acid 3 × 10.sup.-6 M Pyridoxal HCl 1 × 10.sup.-5 M Pyridoxine HCl 3 × 10.sup.-7 M Riboflavin 8 × 10.sup.-7 M Thiamine HCl 9 × 10.sup.-6 M Vitamin B12 3 × 10.sup.-7 M D-Glucose 2 × 10.sup.-2 M Na Pyruvate 1 × 10.sup.-3 M Adenine 1 × 10.sup.-6 M Hypoxanthine (Na.sup.+) 7 × 10.sup.-6 M Thymidine HCl 1 × 10.sup.-5 M Choline chloride 1 × 10.sup.-4 M Ethanolamine HCl 2 × 10.sup.-5 M i-Inositol 1 × 10.sup.-4 M Linoleic acid 1 × 10.sup.-7 M Lipoic acid 2 × 10.sup.-7 M HEPES 2.5 × 10.sup.-2 M NaOH 1.23 × 10.sup.-2 M NaHCO.sub.3 3 × 10.sup.-3 M Insulin 8.4 × 10.sup.-7 M Modified Polyurethane 1.7 × 10.sup.-6 M Synthetic Growth Factor comprising oxyethylene- based diols or polyol units on which at least some of the hydroxy groups have been capped with polyisocyanate wherein the isocyanates of said polyisocyanates have been modified by an isocyanate (NCO) reactive functional group and wherein said modified polymer has a free functional group consisting of sulfhydryl or amino. ______________________________________