Enhanced growth medium and method for culturing human mammary epithelial cells

Claim 3

A method for passaging the epithelial cells obtained by the steps of claim 1 to obtain a clonal culture, said method comprising the additional steps of: separating individual cells from the clumps of epithelial cells; and plating individual cells onto a substrate in a medium including conditioned media obtained from cultures of cells selected from the group consisting of human fetal intestine epithelial cells and human bladder epithelial cells so that the cells proliferate in isolation from one another to form colonies.

Claim 4

An assay for determining the toxicity of a particular agent to human mammary epithelial cells, wherein viable colonies of human mammary epithelial cells are prepared by: digesting samples of human mammary tissue with an enzyme digestion mixture including at least one enzyme selected to break down the mammary tissue into clumps of epithelial cells substantially free from attached stromal cells; separating the clumps of epithelial cells from the stromal cells and other cellular material; and, culturing the clumps of epithelial cells in a medium including conditioned media obtained from cultures of cells selected from the group consisting of human fetal intestine epithelial cells and human bladder epithelial cells so that the cells in the clumps proliferate; separating individual cells from the clumps of epithelial cells; and plating a relatively small number of the individual cells onto a substrate in a medium including conditioned media from at least one media obtained from human fetal intestine epithelial cells and human bladder epithelial cells so that the cells proliferate in isolation from one another to form colonies; said assay comprising: exposing the viable colonies to the particular agent; and determining the survival rate of the colonies.

Claim 5

An assay for determining the toxicity of a particular agent to human mammary epithelial cells, wherein viable colonies of human mammary epithelial cells are prepared by: digesting samples of human mammary tissue with an enzyme digestion mixture including at least one enzyme selected to break down the mammary tissue into clumps of epithelial cells substantially free from attached stromal cells; separating the clumps of epithelial cells from the stromal cells and other cellular material; and, culturing the clumps of epithelial cells in a medium including conditioned media obtained from cultures of cells selected from the group consisting of human fetal intestine epithelial cells and human bladder epithelial cells so that the cells in the clumps proliferate; separating individual cells from the clumps of epithelial cells; and plating a relatively small number of the individual cells onto a substrate in a medium including conditioned media from at least one media obtained from human fetal intestine epithelial cells and human bladder epithelial cells so that the cells proliferate in isolation from one another to form colonies; said assay comprising: exposing the viable colonies to the particular agent; and determining the survival rate of the colonies.

Claim 6

A method as in claim 1, wherein the step of digesting the tissue is carried out with agitation and at a temperature in the range from approximately 20° to 37° C. and wherein said digestion step is terminated when clumps of the cells are observed to be formed.

Claim 7

A method as in claim 2, wherein the step of separating individual cells from the clumps of epithelial cells comprises treating the clumps with a mixture including trypsin.

Claim 8

A method as in claim 2, wherein the substrate is plated with feeder cells prior to culturing the epithelial cells.

Claim 9

A method as in claim 6, wherein the feeder cells are normal human skin fibroblasts.

Claim 10

An assay for determining the toxicity of a particular agent to human mammary epithelial cells, said assay employing a culture of human mammary epithelial cells prepared by: digesting samples of human mammary tissue with an enzyme digestion mixture including at least one enzyme selected to break down the mammary tissue into clumps of epithelial cells substantially free from attached stromal cells; separating the clumps of epithelial cells from the stromal cells and other cellular material; and culturing the clumps of epithelial cells in a medium including conditioned media obtained from cultures of cells selected from the group consisting of human fetal intestine epithelial cells and human bladder epithelial cells so that the cells in the clumps proliferate; separating individual cells from the clumps of epithelial cells; and plating individual cells onto a substrate in a medium including conditioned media from at least one of the media obtained from human fetal intestine epithelial cells and human bladder epithelial cells so that the cells proliferate in isolation from one another to form colonies, wherein said substrate has been plated with feeder cells prior to culturing the epithelial cells; said assay comprising: exposing the plated cells to the agent; terminating the exposure of the colonies to the treatment; providing supplemental growth media further including feeder cells; allowing the colonies plated on the substrate to proliferate until it is possible to distinguish those colonies which continue to grow from those colonies which have ceased to grow prior to determining the survival rate; and determining the toxicity by comparing the number of viable colonies with the number of colonies which have ceased to grow.

Claim 11

An assay for determining the toxicity of a particular agent to human mammary epithelial cells, said assay employing a culture of human mammary epithelial cells prepared by: digesting samples of human mammary tissue with an enzyme digestion mixture including at least one enzyme selected to break down the mammary tissue into clumps of epithelial cells substantially free from attached stromal cells; separating the clumps of epithelial cells from the stromal cells and other cellular material; and culturing the clumps of epithelial cells in a medium including conditioned media obtained from cultures of cells selected from the group consisting of human fetal intestine epithelial cells and human bladder epithelial cells so that the cells in the clumps proliferate; separating individual cells from the clumps of epithelial cells; and plating individual cells onto a substrate in a medium including conditioned media from at least one of the media obtained from human fetal intestine epithelial cells and human bladder epithelial cells so that the cells proliferate in isolation from one another to form colonies, wherein said substrate has been plated with feeder cells prior to culturing the epithelial cells; said assay comprising: exposing the plated cells to the agent; terminating the exposure of the colonies to the treatment; providing supplemental growth media further including feeder cells; allowing the colonies plated on the substrate to proliferate until it is possible to distinguish those colonies which continue to grow from those colonies which have ceased to grow prior to determining the survival rate; and determining the toxicity by comparing the number of viable colonies with the number of colonies which have ceased to grow.

Claim 12

A growth medium, comprising insulin, cholera toxin and conditioned medium obtained from a culture of cells selected from the group consisting of human fetal intestine epithelial cells and human bladder epithelial cells, said components being present in basal medium at concentrations effective to promote the growth of human mammary epithelial cells therein.

Claim 13

A growth medium, comprising insulin, cholera toxin and conditioned medium obtained from a culture of cells selected from the group consisting of human fetal intestine epithelial cells and human bladder epithelial cells, said components being present in basal medium at concentrations effective to promote the growth of human mammary epithelial cells therein.

Claim 14

A growth medium, as in claim 9 wherein the insulin is present at a concentration in the range from approximately 0.1 to 20 μg/ml.

Claim 15

A growth medium, as in claim 9 wherein the cholera toxin is present at a concentration in the range from approximately 0.1 to 1000 ng/ml.

Claim 16

A growth medium, as in claim 9 wherein the conditioned media is present at a concentration in the range from approximately 10 to 50 weight percent.

Claim 17

A growth medium, as in claim 9 including additional components selected from the group consisting of epidermal growth factor at a concentration not exceeding approximately 20 ng/ml, triiodothyronine at a concentration not exceeding approximately 10 -7 M, estradiol at a concentration not exceeding 10 -7 M, and serum not exceeding 3% by weight.

Claim 18

A viable culture of human mammary epithetial cells in a basal medium comprising said cells, insulin, cholera toxin and conditioned medium obtained from a culture of cells selected from the group consisting of human fetal instestine epithelial cells and human bladder epithelial cells wherein said insulin, cholera toxin and conditioned medium are present in amounts sufficient to promote the growth of human mammary epithetial cells.

Claim 19

A viable culture of human mammary epithetial cells in a basal medium comprising said cells, insulin, cholera toxin and conditioned medium obtained from a culture of cells selected from the group consisting of human fetal instestine epithelial cells and human bladder epithelial cells wherein said insulin, cholera toxin and conditioned medium are present in amounts sufficient to promote the growth of human mammary epithetial cells.