US4851346A
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US
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Chemically defined growth medium
Assignee: Bayer Corp
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Filing: 1987-12-21
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Granted: 1989-07-25
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Expires: 2007-12-21
Abstract
Abstract A growth medium suitable for increasing the yield of plasminogen activator obtained from plasminogen activator-producing cells. The growth medium is composed of a 1:1:1 (by weight) mixture of Waymouth's MB752/1, Dulbecco MEM and Ham's F-12. The growth medium is preferably supplemented with growth factors.
Inventors
- 👤 Sham Y. Chan
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 21:36
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6424; C12N9/6456; C12N1/00; C12R; C12R2001/00; C12R2001/91
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
25 words
A chemically defined growth medium designated as SYC medium, consisting essentially of a 1:1:1: (by weight) mixture of Waymouth's MB752/1, Dulbecco MEM and Ham's F-12.
Claim 2
Dependent Claim
25 words
A chemically defined growth medium designated as SYC medium, consisting essentially of a 1:1:1: (by weight) mixture of Waymouth's MB752/1, Dulbecco MEM and Ham's F-12.
Claim 3
Dependent Claim
19 words
The chemically defined growth medium of claim 1 supplemented with fetuin, bovine serum albumin, insulin, transferrin, 5α-dihydrotestosterone and dexamethasone.
Claim 4
Dependent Claim
73 words
The chemically defined growth medium of claim 2 supplemented with fetuin in a concentration of from 50-150 milligrams per liter; bovine serum albumin in a concentration of from 50-150 milligrams per liter; insulin in a concentration of from 5-10 milligrams per liter; transferrin in a concentration of from 5-40 milligrams per liter; 5α-dihydrotestosterone in a concentration of from 50-200 micrograms per liter; and dexamethasone in a concentration of from 50-200 micrograms per liter.
Claim 5
Dependent Claim
67 words
The chemically defined growth medium of claim 3 supplemented with fetuin in a concentration of 75 milligrams per liter; bovine serum albumin in a concentration of 75 milligrams per liter; insulin in a concentration of 8 milligrams per liter; transferrin in a concentration of 25 milligrams per liter; 5α-dihydrotestosterone in a concentration of 100 micrograms per liter; and dexamethasone in a concentration of 100 micrograms per liter.
References Cited (Backward Citations)
US4443546A
Cited
US4560655A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-07-24 | 1993-01-23 | 1993-07-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-07-24 | 1997-01-23 | 1997-07-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-07-24 | 2001-01-23 | 2001-07-25 | $7700.00 | Window Closed / Lapsed |