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US5134057A
Expired / Lapsed
US
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Method of providing a substrate with a layer comprising a polyvinyl based hydrogel and a biochemically active material
Assignee: Drager Nederland BV
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Filing: 1989-10-10
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Granted: 1992-07-28
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Expires: 2009-10-10
Abstract
Abstract Disclosed is a method of providing a substrate with a layer comprising a polyvinyl base hydrogel and a biochemically active material by photolithograhy, comprising coating the substrate by centrifugal force with an aqueous solution of a photosensitive hydrogel forming polymer, a crosslinking agent and a biochemically active material, drying said coating, exposing the coated substrate through a photomask to ultraviolet radiation and developing said exposed coating, which is characterized by applying to the substrate an aqueous solution comprising as the cross-linking agent a polyazonium compound and glutardialdehyde. Because of this specific combination of cross-linking agents superior properties with respect to the binding of the biochemically active material are obtained.
Inventors
- 👤 Martinus H. Kuypers
- 👤 Gerardus F. J. Steeghs
- 👤 Egbert Brinkmann
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 06:49
CPC Classifications
G; G03; G03F; G03F7/00; G03F7/004; G03F7/016; A; A61; A61L; A61L33/00; A61L33/0005; A61L33/0011; A61L33/0029
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
129 words
Method of providing a substrate with a layer comprising a polyvinyl based hydrogel and a biochemically active material by photolithography, comprising combining a preformed first aqueous solution of a photosensitive hydrogel forming polymer and a polyazonium compound as a first cross linking agent with a preformed second aqueous solution of a biochemically active material and glutardialdehyde as a second cross linking agent to form a combination aqueous solution of the hydrogel forming polymer, the biochemically active material and the combination of the polyazonium compound and glutardialdehyde as cross linking agents, coating the substrate by centrifugal force with said combination aqueous solution, drying the coating, exposing the coated substrate through a photomask to ultraviolet radiation and developing the exposed coating by dissolving the non-irradiated parts to form a negative image.
Claim 2
Dependent Claim
129 words
Method of providing a substrate with a layer comprising a polyvinyl based hydrogel and a biochemically active material by photolithography, comprising combining a preformed first aqueous solution of a photosensitive hydrogel forming polymer and a polyazonium compound as a first cross linking agent with a preformed second aqueous solution of a biochemically active material and glutardialdehyde as a second cross linking agent to form a combination aqueous solution of the hydrogel forming polymer, the biochemically active material and the combination of the polyazonium compound and glutardialdehyde as cross linking agents, coating the substrate by centrifugal force with said combination aqueous solution, drying the coating, exposing the coated substrate through a photomask to ultraviolet radiation and developing the exposed coating by dissolving the non-irradiated parts to form a negative image.
Claim 3
Dependent Claim
26 words
Method of claim 1 wherein the polyazonium compound is a monomeric or polymeric organic compound comprising at least two side chain and/or terminal diazonium salt groups.
Claim 4
Dependent Claim
49 words
Method of claim 2 wherein the polyazonium compound is a member selected from the group consisting of a benzidine tetrazonium salt, a diethyl benzidine tetrazonium salt, a dichloro benzidine tetrazonium salt, an o-tolidine tetrazonium salt, an o-dianisidine tetrazonium salt and a reaction product of diphenylamine diazonium sulfate with formaldehyde.
Claim 5
Dependent Claim
15 words
Method of claim 1 wherein the hydrogel forming polymer is polyvinyl alcohol or polyvinyl pyrrolidone.
Claim 6
Dependent Claim
24 words
Method of claim 1 wherein the biochemically active material is a member selected from the group consisting of a protein, proteinous material and polysaccharide.
Claim 7
Dependent Claim
17 words
Method of claim 5 wherein the member of said group is an anti-thrombogenic material or an enzyme.
Claim 8
Dependent Claim
10 words
Method of claim 6 wherein the anti-thrombogenic material is heparin.
Claim 9
Dependent Claim
27 words
Method of claim 6 wherein the enzyme is an enzyme catalyzing a biochemical reaction in which hydrogen peroxide is formed, or in which the pH is changed.
Claim 10
Dependent Claim
28 words
Method of claim 8 wherein the enzyme is an enzyme catalyzing a biochemical reaction in which hydrogen peroxide is formed, and the enzyme is glucose oxidase or alcoholase.
Claim 11
Dependent Claim
88 words
Method of claim 1 wherein the combination aqueous solution is a solution in water or in saline of 0.9% by weight of NaCl, comprising 0.5 to 12% by weight of the hydrogel forming polymer and, based upon 100 parts by weight of the hydrogel forming polymer, 1 to 3 parts by weight of the polyazonium compound, 0.5 to 10 parts by weight of glutardialdehyde, and up to 20 parts by weight of heparin or up to 10 parts by weight of glucose oxidase as the biochemically active material.
Claim 12
Dependent Claim
78 words
Method of claim 10 wherein the combination aqueous solution is a solution in water or in saline of 0.9% by weight of NaCl, comprising 0.5 to 12% by weight of the hydrogel forming polymer and, based upon 100 parts by weight of the hydrogel forming polymer, 2 to 2.5 parts by weight of the polyazonium compound, 5 to 8.5 parts by weight of glutardialdehyde, and 2 to 15 parts by weight of heparin as the biochemically active material.
Claim 13
Dependent Claim
79 words
Method of claim 10 wherein the combination aqueous solution is a solution in water or in saline of 0.9% by weight of NaCl, comprising 0.5 to 12% by weight of the hydrogel forming polymer and, based upon 100 parts by weight of the hydrogel forming polymer, 2 to 2.5 parts by weight of the polyazonium compound, 5 to 8.5 parts by weight of glutardialdehyde, and 2 to 4 parts by weight of glucose oxidase as the biochemically active material.
Claim 14
Dependent Claim
20 words
Method of claim 10 wherein the combination aqueous solution comprises 5 to 9% by weight of the hydrogel forming polymer.
Claim 15
Dependent Claim
19 words
Method of claim 10 wherein the combination aqueous solution comprises about 7% by weight of the hydrogel forming polymer.
Claim 16
Dependent Claim
13 words
Method of claim 1 wherein the substrate is part of a biochemical sensor.
Claim 17
Dependent Claim
12 words
Method of claim 15 wherein the substrate is made of non-conductive material.
Claim 18
Dependent Claim
15 words
Method of claim 16 wherein the substrate is made of silicon, alumina, silica or glass.
Claim 19
Dependent Claim
54 words
Method of claim 15 wherein the substrate is first provided with a base layer by applying an aqueous solution comprising a photosensitive hydrogel forming polymer, and a polyazonium compound and glutardialdehyde as cross linking agents, to form a base coating on the substrate, and thereafter coating onto the base coating said combination aqueous solution.
Claim 20
Dependent Claim
67 words
Method of claim 18 wherein the aqueous solution used to form the base layer is a solution in water or in saline of 0.9% by weight of NaCl, comprising 0.5 to 12% by weight of the hydrogel forming polymer and, based upon 100 parts by weight of the hydrogel forming polymer, 2 to 3 parts by weight of the polyazonium compound and >0.5 by weight of glutardialdehyde.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-07-28 | 1996-01-27 | 1996-07-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-07-28 | 2000-01-27 | 2000-07-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-07-28 | 2004-01-27 | 2004-07-28 | $7700.00 | Window Closed / Lapsed |