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US5133908A
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Process for the preparation of dispersible colloidal systems of a substance in the form of nanoparticles
Assignee: Centre National de la Recherche Scientifique
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Filing: 1989-06-30
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Granted: 1992-07-28
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Expires: 2009-06-30
Abstract
Abstract The Process according to the invention comprises: (1) the preparation of a liquid phase consisting essentially of a solution of the substance in a solvent or in a mixture of solvents to which may be added one or more surfactants, (2) the preparation of a second liquid phase consisting essentially of a non-solvent of a mixture of non-solvents for the substance and to which may be added one or more surfactants, the non-solvent or the mixture of non-solvents for the substance being miscible in all proportions with the solvent or the mixture of solvents for the substance, (3) the addition of one of the liquid phases prepared in (1) or (2) to the other with moderate stirring so as to produce a colloidal suspension of nanoparticles of the substance and, (4) if desired, the removal of all or part of the solvent or the mixture of solvents for the substance and of the non-solvent or the mixture of non-solvents for the substance so as to produce a colloidal suspension of nanoparticles of the desired concentration or to produce a powder of nanoparticles. Said substance may be a protein. Field of use: chemistry, biochemistry, pharmacy, medicine, cosmetics.
Inventors
- 👤 Serge Stainmesse
- 👤 Hatem Fessi
- 👤 Jean-Phillppe Devissaguet
- 👤 Francis Puisieux
- 👤 Curt Theis
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 20:15
CPC Classifications
A; A61; A61K; A61K9/00; A61K9/48; A61K9/50; A61K9/51; A61K9/5107; A61K9/513; A61K9/5169; A61K8/00; A61K8/02; A61K8/0241; A61K8/18; A61K8/30; A61K8/64; A61K8/65; A61K9/5192; A61Q; A61Q19/00; B; B82; B82Y; B82Y5/00; C; C08; C08J; C08J3/00; C08J3/12; C08J3/14; A61K2800/00; A61K2800/40; A61K2800/41; A61K2800/413; A61K2800/56; Y; Y10; Y10S; Y10S977/00; Y10S977/70; Y10S977/773; Y10S977/778; Y10S977/786; Y10S977/84
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
144 words
A process for the preparation of dispersible colloidal systems of a protein in the form of spherical particles of the matrix type and of a size less than 500 nm (nanoparticles), comprising: combining (1) a first liquid phase consisting essentially of a solution of the protein in water or in an aqueous mixture, said first liquid phase being at a temperature lower than the coagulation temperature of the protein; and (2) a greater volume of a second liquid phase consisting essentially of water or an aqueous mixture at a temperature higher than the coagulation temperature of the protein, which may contain a biologically active substance and to which may be added one or more surfactants; thereby substantially instantaneously to precipitate from said first liquid phase and said second liquid phase particles of said protein to produce a colloidal suspension of nanoparticles of the protein.
Claim 2
Dependent Claim
144 words
A process for the preparation of dispersible colloidal systems of a protein in the form of spherical particles of the matrix type and of a size less than 500 nm (nanoparticles), comprising: combining (1) a first liquid phase consisting essentially of a solution of the protein in water or in an aqueous mixture, said first liquid phase being at a temperature lower than the coagulation temperature of the protein; and (2) a greater volume of a second liquid phase consisting essentially of water or an aqueous mixture at a temperature higher than the coagulation temperature of the protein, which may contain a biologically active substance and to which may be added one or more surfactants; thereby substantially instantaneously to precipitate from said first liquid phase and said second liquid phase particles of said protein to produce a colloidal suspension of nanoparticles of the protein.
Claim 3
Dependent Claim
13 words
The process according to claim 1, wherein the protein is a serum albumin.
Claim 4
Dependent Claim
12 words
The process according to claim 1, wherein the protein is an elastin.
Claim 5
Dependent Claim
17 words
The process according to claim 1, wherein said first liquid phase further comprises a biologically active substance.
Claim 6
Dependent Claim
25 words
The process according to claim 4, wherein the combining step is performed such that the biologically active substance is bound to the nanoparticles of protein.
Claim 7
Dependent Claim
40 words
The process according to claim 1, further comprising removing at least part of the water or aqueous mixture in said colloidal suspension, so as to form a colloidal suspension of a desired concentration or to form a powder of nanoparticles.
Claim 8
Dependent Claim
19 words
The process according to claim 6, wherein all of the water in the colloidal suspension is removed by lyophilization.
Claim 9
Dependent Claim
20 words
The process according to claim 1, wherein the first liquid phase has a temperature of from 0° to 50° C.
Claim 10
Dependent Claim
20 words
The process according to claim 1, wherein the second liquid phase has a temperature of from 80° to 100° C.
Claim 11
Dependent Claim
30 words
The process according to claim 1, wherein the pH of the combined first and second liquid phases is two to three units removed from the isoelectric point of the protein.
Claim 12
Dependent Claim
23 words
The process according to claim 1, wherein the protein is present in the first liquid phase in a concentration of 0.1 to 10%.
Claim 13
Dependent Claim
15 words
The process according to claim 11, wherein the protein concentration is from 0.5 to 4%.
Claim 14
Dependent Claim
24 words
The process according to claim 1, wherein the ratio volumes of the first liquid phase to the second liquid phase is at least 0.1.
Claim 15
Dependent Claim
14 words
The process according to claim 13, wherein the ratio is from 0.1 to 0.6.
Claim 16
Dependent Claim
18 words
The process according to claim 1, wherein the nanoparticles have a size of about 150 to 300 nm.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-07-28 | 1996-01-27 | 1996-07-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-07-28 | 2000-01-27 | 2000-07-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-07-28 | 2004-01-27 | 2004-07-28 | $7700.00 | Window Closed / Lapsed |