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US5131850A Expired / Lapsed US ⏱️ 0.0 years remaining

Method for cryopreserving musculoskeletal tissues

Assignee: Artivion Inc
•
Filing: 1989-11-03
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Granted: 1992-07-21
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Expires: 2009-11-03
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Abstract

Abstract Disclosed herein is a method for cryopreserving musculoskeletal tissues, such as ligaments, tendons and cartilage, by placing such tissue in contact with a composition containing a cryopreserving agent comprising a cell-penetrating organic solute, which is preferably dimethylsulfoxide, and a glycosaminoglycan, which is preferably chondroitin sulphate, in an amount sufficient to cryopreserve the musculoskeletal tissue. The addition of a glycosaminoglycan to a cryopreserving agent comprising a cell-penetrating organic solute permits a broad range of cooling rates to be employed, rather than the very narrow ranges which are employed using a cryopreserving agent comprising a cell-penetrating organic solvent without the glycosaminoglycan. Also disclosed are a freezing schedule designed to maximize retention of tissue cell viability and biomechanical properties during and after the freezing process, and a thawing schedule which maximizes cell viability.

Inventors

  • 👤 Kelvin G. M. Brockbank

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-10-23 13:39
CPC Classifications
A; A01; A01N; A01N1/00; A01N1/10; A01N1/12; A01N1/122; A01N1/125

Claims Analysis

Total Claims: 10
Claim 1
Independent Claim 48 words
A method of cryopreserving musculoskeletal tissue comprising placing the tissue in contact with a cryopreserving agent comprising a cell-penetrating organic solute and a glycosaminoglycan, in an amount sufficient to cryopreserve the musculoskeletal tissue, and maintaining the musculoskeletal tissue in contact with the cryopreserving agent in a frozen state.
Claim 2
Independent Claim 48 words
A method of cryopreserving musculoskeletal tissue comprising placing the tissue in contact with a cryopreserving agent comprising a cell-penetrating organic solute and a glycosaminoglycan, in an amount sufficient to cryopreserve the musculoskeletal tissue, and maintaining the musculoskeletal tissue in contact with the cryopreserving agent in a frozen state.
Claim 3
Dependent Claim 24 words
The method of claim 1, wherein the musculoskeletal tissue is placed in contact with the cryopreserving agent which comprises chondroitin sulphate as the glycosaminoglycan.
Claim 4
Dependent Claim 31 words
The method of claim 1, wherein the musculoskeletal tissue is placed in contact with the cryopreserving agent which comprises the glycosaminoglycan in a concentration of from about 1% to about 10%.
Claim 5
Dependent Claim 31 words
The method of claim 3, wherein the musculosketal tissue is placed in contact with the cryopreserving agent which comprises the glycosaminoglycan in a concentration of from about 2.5% to about 5%.
Claim 6
Dependent Claim 27 words
The method of claim 4, wherein the musculoskeletal tissue is placed in contact with the cryopreserving agent which comprises the glycosaminoglycan in a concentration of about 2.5%.
Claim 7
Dependent Claim 25 words
The method of claim 1, wherein the musculoskeletal tissue is placed in contact with the cryopreserving agent which comprises dimethylsulphoxide as the cell-penetrating organic solute.
Claim 8
Dependent Claim 33 words
The method of claim 1, wherein the musculoskeletal tissue is placed in contact with the cryopreserving agent which comprises the cell-penetrating organic solute in a concentration of from about 0.5M to about 3M.
Claim 9
Dependent Claim 33 words
The method of claim 7, wherein the musculoskeletal tissue is placed in contact with the cryopreserving agent which comprises the cell-penetrating organic solute in a concentration of from about 0.75M to about 1.5M.
Claim 10
Dependent Claim 28 words
The method of claim 8, wherein the musculoskeletal tissue is placed in contact with the cryopreserving agent which comprises the cell-penetrating organic solute in a concentration of 1M.

References Cited (Backward Citations)

US3033662A Cited
US3444039A Cited
US4486416A Cited
US4959319A Cited
US4800429A Cited

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1995-07-21 1996-01-20 1996-07-21 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 1999-07-21 2000-01-20 2000-07-21 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 2003-07-21 2004-01-20 2004-07-21 $7700.00 Window Closed / Lapsed

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