Patent US5130239A successfully refreshed from Google Patents.
US5130239A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for preparing lactosucrose high-content power
Assignee: ENSUIKO SEITO KK
•
Filing: 1991-03-07
•
Granted: 1992-07-14
•
Expires: 2011-03-07
Abstract
Abstract The lactosucrose high-content powder according to the present invention is prepared by allowing an aqueous solution containing sucrose and lactose to act on a saccharide-transferring enzyme, removing concomitant saccharides in the resultant saccharide solution containing lactosucrose to obtain a lactosucrose high-content solution with a lactosucrose content of 45 w/w % or higher on sugar composition, and spray-drying the resultant solution to obtain a lactosucrose high-content powder. The powder is incorporated in an orally- or parenterally-administrable product to obtain an orally- or parenterally-administrable product which exerts a selective growth-promoting-effect on a microorganism of the genus Bifidobacterium in vivo.
Inventors
- 👤 Kozo Hara
- 👤 Kohki Fujita
- 👤 Masayuki Yamashita
- 👤 Yasuhiko Tsunetomi
- 👤 Shuzo Sakai
- 👤 Toshio Miyake
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 08:06
CPC Classifications
A; A23; A23G; A23G4/00; A23G4/06; A23G4/10; C; C12; C12P; C12P19/00; A23C; A23C9/00; A23C9/152; A23C9/156; A23F; A23F5/00; A23F5/24; A23F5/243; A23G1/00; A23G1/30; A23G1/56; A23G3/00; A23G3/34; A23G3/346; A23K; A23K20/00; A23K20/10; A23K20/163; A23L; A23L27/00; A23L27/30; A23L27/33; A23L9/00; A23L9/10; C12P19/18; C12Y; C12Y204/00; C12Y204/01; C12Y204/0101; C12Y302/00; C12Y302/01; C12Y302/01026; C13; C13K; C13K13/00; A23G2200/00; A23G2200/06; Y; Y10; Y10S; Y10S435/00; Y10S435/803; Y10S435/8215; Y10S435/822; Y10S435/83
Claims Analysis
Total Claims: 32
Claim 1
Independent Claim
93 words
A process for preparing a lactosucrose high-content powder, which comprises: (a) allowing a saccharide-transferring enzyme to act on an aqueous solution containing sucrose and lactose, wherein the ratio of said sucrose and lactose is in the range of about 1:10 to 10:1; (b) removing concomitant saccharides from the resultant saccharide solution containing lactosucrose to obtain a lactosucrose high-content solution with a lactosucrose content of 45 w/w %, d.s.b., or higher or sugar composition; and (c) spray-drying the lactosucrose high-content solution into a free-flowing powder having a moisture content of lower than about 3%.
Claim 2
Dependent Claim
93 words
A process for preparing a lactosucrose high-content powder, which comprises: (a) allowing a saccharide-transferring enzyme to act on an aqueous solution containing sucrose and lactose, wherein the ratio of said sucrose and lactose is in the range of about 1:10 to 10:1; (b) removing concomitant saccharides from the resultant saccharide solution containing lactosucrose to obtain a lactosucrose high-content solution with a lactosucrose content of 45 w/w %, d.s.b., or higher or sugar composition; and (c) spray-drying the lactosucrose high-content solution into a free-flowing powder having a moisture content of lower than about 3%.
Claim 3
Dependent Claim
27 words
The process of claim 1, wherein a total concentration of sucrose and lactose in the step (a) is in the range of about 10-60 w/w %, d.s.b.
Claim 4
Dependent Claim
32 words
The process of claim 1, wherein a pH and a temperature in the step (a) are a pH in the range of 3-9 and a temperature in the range of 5°-70° C.
Claim 5
Dependent Claim
31 words
The process of claim 1, wherein said saccharide-transferring enzyme in the step (a) is a member selected from the group consisting of a fructose-transferring enzyme, a galactose-transferring enzyme, and mixtures thereof.
Claim 6
Dependent Claim
13 words
The process of claim 6, wherein said fructose-transferring enzyme is levansucrase or β-D-fructofuranosidase.
Claim 7
Dependent Claim
19 words
The process of claim 5, wherein said levansucrase is derived from a microorganism of the genus Bacillus or Aerobacter.
Claim 8
Dependent Claim
17 words
The process of claim 5, wherein said β-D-fructofuranosidase is derived from a microorganism of the genus Arthrobacter.
Claim 9
Dependent Claim
15 words
The process of claim 7, wherein said microorganism is of Arthrobacter sp. K-1 (FERM P-10736).
Claim 10
Dependent Claim
20 words
The process of claim 4, wherein said galactosetransferring enzyme is derived from a microorganism of the genus Sporoboromyces or Rahnella.
Claim 11
Dependent Claim
52 words
The process of claim 1, wherein the step (b) comprises one or more methods selected from the group consisting of a column chromatography using a strongly-acidic cation exchange resin of alkali-metal- or alkaline-earth metal-form, a fermentation method using an invertase-defective yeast, and a method to remove a concomitant lactose by crystallizing it.
Claim 12
Dependent Claim
24 words
The process of claim 10, wherein said column chromatography is effected by setting a gel-bed depth of said resin to at least 3 m.
Claim 13
Dependent Claim
20 words
The process of claim 10, wherein said column chromatography is effected by using two or more columns cascaded in series.
Claim 14
Dependent Claim
28 words
The process of claim 10, wherein said column chromatography is effected by setting the inner temperature of said column to a temperature in the range of 30°-70° C.
Claim 15
Dependent Claim
20 words
The process of claim 1, wherein the step (c) comprises a method using a rotating-disc, pressure nozzle, or twin-fluid nozzle.
Claim 16
Dependent Claim
91 words
A process for preparing an orally- or parenterally-administrable product, which comprises: (a) allowing a saccharide-transferring enzyme to act on an aqueous solution containing sucrose and lactose; (b) removing concomitant saccharides from the resultant saccharide solution containing lactosucrose to obtain a lactosucrose high-content solution with a lactosucrose content of 45 w/w %, d.s.b, or higher on sugar composition; (c) spray-drying the lactosucrose high-content solution into a free-flowing powder solution having a moisture content of lower than about 3%; and (d) incorporating the resultant lactosucrose high-content powder in an orally- or parenterally-administrable product.
Claim 17
Dependent Claim
91 words
A process for preparing an orally- or parenterally-administrable product, which comprises: (a) allowing a saccharide-transferring enzyme to act on an aqueous solution containing sucrose and lactose; (b) removing concomitant saccharides from the resultant saccharide solution containing lactosucrose to obtain a lactosucrose high-content solution with a lactosucrose content of 45 w/w %, d.s.b, or higher on sugar composition; (c) spray-drying the lactosucrose high-content solution into a free-flowing powder solution having a moisture content of lower than about 3%; and (d) incorporating the resultant lactosucrose high-content powder in an orally- or parenterally-administrable product.
Claim 18
Dependent Claim
27 words
The process of claim 15, wherein a total concentration of sucrose and lactose in the step (a) is in the range of about 10-60 w/w %, d.s.b.
Claim 19
Dependent Claim
22 words
The process of claim 16, wherein a ratio of said sucrose and lactose is in the range of about 1:10 to 10:1.
Claim 20
Dependent Claim
32 words
The process of claim 15, wherein a pH and a temperature in the step (a) are a pH in the range of 3-9 and a temperature in the range of 5°-70° C.
Claim 21
Dependent Claim
31 words
The process of claim 15, wherein said saccharide-transferring enzyme in the step (a) is a member selected from the group consisting of a fructose-transferring enzyme, a galactose-transferring enzyme, and mixtures thereof.
Claim 22
Dependent Claim
13 words
The process of claim 22, wherein said fructose-transferring enzyme is levansucrase or β-D-fructofuranosidase.
Claim 23
Dependent Claim
19 words
The process of claim 20, wherein said levansucrase is derived from a microorganism of the genus Bacillus or Aerobacter.
Claim 24
Dependent Claim
17 words
The process of claim 20, wherein said β-D-fructofuranosidase is derived from a microorganism of the genus Arthrobacter.
Claim 25
Dependent Claim
15 words
The process of claim 22, wherein said microorganism is of Arthrobacter sp. K-1 (FERM P-10736).
Claim 26
Dependent Claim
20 words
The process of claim 19, wherein said galactosetransferring enzyme is derived from a microorganism of the genus Sporoboromyces or Rahnella.
Claim 27
Dependent Claim
52 words
The process of claim 15, wherein the step (b) comprises one or more methods selected from the group consisting of a column chromatography using a strongly-acidic cation exchange resin of alkali-metal- or alkaline-earth metal-form, a fermentation method using an invertase-defective yeast, and a method to remove a concomitant lactose by crystallizing it.
Claim 28
Dependent Claim
24 words
The process of claim 25, wherein said column chromatography is effected by setting a gel-bed depth of said resin to at least 3 m.
Claim 29
Dependent Claim
20 words
The process of claim 25, wherein said column chromatography is effected by using two or more columns cascaded in series.
Claim 30
Dependent Claim
28 words
The process of claim 25, wherein said column chromatography is effected by setting the inner temperature of said column to a temperature in the range of 30°-70° C.
Claim 31
Dependent Claim
20 words
The process of claim 15, wherein the step (c) comprises a method using a rotating-disc, pressure nozzle, or twin-fluid nozzle
Claim 32
Dependent Claim
18 words
The process of claim 15, wherein said product is a food product, feed, pet food, pharmaceutical or cosmetic.
References Cited (Backward Citations)
JPS5758905A
Cited
US4431737A
Cited
JPS5953835A
Cited
US4617269A
Cited
US4859488A
Cited
JPH0235095A
Cited
JPH0327285A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-07-14 | 1996-01-13 | 1996-07-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-07-14 | 2000-01-13 | 2000-07-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-07-14 | 2004-01-13 | 2004-07-14 | $7700.00 | Window Closed / Lapsed |