US5108733A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Stabilized trinder reagent
Assignee: Instrumentation Laboratory SpA
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Filing: 1990-08-30
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Granted: 1992-04-28
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Expires: 2010-08-30
Abstract
Abstract Spontaneous aspecific coloration in so-called Trinder reagents which can alter subsequent colorimetric determinations is inhibited by adding compounds of the chelating agent class to the solution. The resultant compositions contain a peroxidase enzyme, a phenylpyrazone derivative, a compound of phenolic or aromatic amine structure and a stabilizer. Preferred stabilizers are ethylenediaminetetraacetic acid (EDTA) and diethylenetriaminepentaacetic acid (DTPA).
Inventors
- 👤 Dario Frontini
- 👤 Maurizio D'Alterio
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 06:48
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/28
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
29 words
A method for inhibiting spontaneous aspecific coloration in a Trinder reagent containing a phenylpyrazone derivative, characterized by adding an effective amount of a chelating agent to the Trinder reagent.
Claim 2
Independent Claim
29 words
A method for inhibiting spontaneous aspecific coloration in a Trinder reagent containing a phenylpyrazone derivative, characterized by adding an effective amount of a chelating agent to the Trinder reagent.
Claim 3
Dependent Claim
22 words
A method as claimed in claim 1, characterized in that the chelating agent used is EDTA (ethylenediaminetetraacetic acid) or a salt thereof.
Claim 4
Dependent Claim
22 words
A method as claimed in claim 1, characterised in that the chelating agent used is DTPA (diethylenetriaminepentaacetic acid) or a salt thereof.
Claim 5
Dependent Claim
25 words
A method as claimed in claim 1, characterized in that the chelating agent is added in a concentration of between 0.005 mM and 50 mM.
Claim 6
Dependent Claim
14 words
The method of claim 4 wherein said concentration is 1 mM to 10 mM.
Claim 7
Dependent Claim
16 words
The method of claim 1 wherein the chelating agent used is a polyaminepolyacetic acid or salt.
References Cited (Backward Citations)
USRE29498E
Cited
US3992158A
Cited
EP0071730A1
Cited
EP0123115A2
Cited
US4716110A
Cited
WO1986004610A1
Cited
EP0230229A2
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-04-28 | 1995-10-28 | 1996-04-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-04-28 | 1999-10-28 | 2000-04-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-04-28 | 2003-10-28 | 2004-04-28 | $7700.00 | Window Closed / Lapsed |