EP0230229A2
Expired / Lapsed
EP
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Stable composition for the determination of peroxidatively active substances
Assignee: Bayer Corp
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Filing: 1987-01-08
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Granted: —
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Expires: 2007-01-08
Abstract
Abstract A stable test device for the determination of a peroxidatively active substance comprising a carrier containing 1,4-diisopropylbenzene dihydroperoxide and a benzidine indicator in a molar ratio of from 0.9 to 3.0. The composition can be incorporated with a carrier matrix with a ferric chelate to provide ascorbate resistance, important in a urine test for occult blood. The test device is stable under storage and heat stress and can be used to prepare a multitest strip containing a glucose test based on a peroxidase/potassium iodide indicator system.
Inventors
- 👤 Ibrahim A. Ismail
- 👤 Teresa Yip
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-02 07:54
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/28; C12Q2326/00; C12Q2326/10; C12Q2326/12; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S436/00; Y10S436/904
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
33 words
A stable test composition for the determination of a peroxidatively active substance in a sample, comprising: 1,4-diisopropylbenzene dihydroperoxide and a benzidine indicator in a molar ratio of from about 0.9 to about 3.0.
Claim 2
Dependent Claim
33 words
A stable test composition for the determination of a peroxidatively active substance in a sample, comprising: 1,4-diisopropylbenzene dihydroperoxide and a benzidine indicator in a molar ratio of from about 0.9 to about 3.0.
Claim 3
Dependent Claim
11 words
The stable composition of claim 1 which additionally includes an enhancer.
Claim 4
Dependent Claim
16 words
The test composition of claim 2 in which the enhancer is chosen from 4-methylquinoline or 6-methoxyquinoline.
Claim 5
Dependent Claim
31 words
The stable test composition of any of claims 1 to 3 which additionally includes a buffer capable of providing a pH in the pH range of from about 4 to 7.5.
Claim 6
Dependent Claim
71 words
A stable test device sensitive to the presence of at least one part hemoglobin in one million parts of a body sample comprising: a) a carrier matrix; and b) incorporated therein a test composition comprising, 1,4-diisopropylbenzene dihydroperoxide and a benzidine indicator in a molar ratio of from about 0.9 to about 3.0; an enhancer, and; a buffer capable of providing a pH in the range from about 4 to about 7.5.
Claim 7
Dependent Claim
71 words
A stable test device sensitive to the presence of at least one part hemoglobin in one million parts of a body sample comprising: a) a carrier matrix; and b) incorporated therein a test composition comprising, 1,4-diisopropylbenzene dihydroperoxide and a benzidine indicator in a molar ratio of from about 0.9 to about 3.0; an enhancer, and; a buffer capable of providing a pH in the range from about 4 to about 7.5.
Claim 8
Dependent Claim
21 words
The test device of claim 5 which additionally includes a ferric chelate capable of providing ascorbate resistance to the test composition.
Claim 9
Dependent Claim
38 words
The test device of claim 6 in which the ferric chelate is the ferric chelate of N-(2-hydroxyethyl)ethylenediamine triacetic acid and the buffer is capable of providing a pH in the range of from about 6.5 to about 7.1.
Claim 10
Dependent Claim
32 words
The test device of any of claims 5 to 7, in which the buffer is a combination of tris(hydroxymethyl)aminomethane-malonic acid and triethanolamine borate, in a molar ratio of about 1 to 3.
Claim 11
Dependent Claim
15 words
The test device of any of claims 5 to 8 which additionally includes polyvinyl pyrrolidine.
Claim 12
Independent Claim
138 words
A method of preparing a stable, ascorbate resistant test device sensitive to the presence of at least one part hemoglobin per million parts of a body fluid sample, comprising the steps of: a) preparing a first solution containing a ferric chelate capable of imparting ascorbate resistance to the test device and a buffer capable of providing a pH in the range of from about 6.5 to about 7.1; b) preparing a second solution containing an organic solvent, 1,4-diisopropylbenzene dihydroperoxide and a benzidine indicator in a molar ratio o f from about 1.6 to 3.0 and an enhancer; c) incorporating a carrier matrix with either the first or second solution; d) drying the incorporated carrier; e) incorporating the dried carrier with the other of the first or second solutions, not previously incorporated; and f) drying the doubly incorporated carrier.
Claim 13
Independent Claim
138 words
A method of preparing a stable, ascorbate resistant test device sensitive to the presence of at least one part hemoglobin per million parts of a body fluid sample, comprising the steps of: a) preparing a first solution containing a ferric chelate capable of imparting ascorbate resistance to the test device and a buffer capable of providing a pH in the range of from about 6.5 to about 7.1; b) preparing a second solution containing an organic solvent, 1,4-diisopropylbenzene dihydroperoxide and a benzidine indicator in a molar ratio o f from about 1.6 to 3.0 and an enhancer; c) incorporating a carrier matrix with either the first or second solution; d) drying the incorporated carrier; e) incorporating the dried carrier with the other of the first or second solutions, not previously incorporated; and f) drying the doubly incorporated carrier.
References Cited (Backward Citations)
No citation records indexed.
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