US5089383A
Expired / Lapsed
US
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Heterogeneous assay having delayed signal production
Assignee: Siemens Healthcare Diagnostics GmbH Germany
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Filing: 1988-12-01
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Granted: 1992-02-18
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Expires: 2008-12-01
Abstract
Abstract Methods and compositions are provided for assays involving members of a specific binding pair ("sbp members") and members of a signal producing system ("sps members"). The signal producing system is capable of producing a detectible signal in relation to the presence or amount of an analyte in a sample suspected of containing the analyte. Exemplary of sps members are enzymes and enzyme substrates, which react with each other to produce a signal. The improvement of the present invention comprises temporarily delaying the production of the signal without subsequent reagent addition. The delay can be achieved by employing an inhibitor which can be an alternate substrate for the enzyme or a compound which reacts with the product of the enzyme and its substrate in an effective amount.
Inventors
- 👤 Sydney Leeder
- 👤 Robert F. Zuk
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 05:44
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/536; G01N33/542; G01N33/543; G01N33/54306; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S435/97; Y10S436/00; Y10S436/807; Y10S436/81
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
121 words
In an assay method involving the reaction of members of a specific binding pair and the reaction of members of a signal producing system capable of producing a detectable signal in relation to the presence or amount of an analyte in the sample suspected of containing said analyte wherein at least one specific binding pair member is bound to at least one signal producing system member, wherein the improvement comprises the step of temporarily delaying the production of said signal by employing an effective amount of an exhaustible signal inhibitor, said signal inhibitor being a compound that interacts with one of said signal producing system members, or a product thereof, to delay production of said signal for a predetermined time period.
Claim 2
Dependent Claim
121 words
In an assay method involving the reaction of members of a specific binding pair and the reaction of members of a signal producing system capable of producing a detectable signal in relation to the presence or amount of an analyte in the sample suspected of containing said analyte wherein at least one specific binding pair member is bound to at least one signal producing system member, wherein the improvement comprises the step of temporarily delaying the production of said signal by employing an effective amount of an exhaustible signal inhibitor, said signal inhibitor being a compound that interacts with one of said signal producing system members, or a product thereof, to delay production of said signal for a predetermined time period.
Claim 3
Dependent Claim
19 words
The method of claim 1 wherein said delay step comprises delaying said reaction of said signal producing system members.
Claim 4
Dependent Claim
42 words
The method of claim 1 wherein said delay step further comprises having an admixture of one or more of the signal producing system members, mixed with said exhaustible signal inhibitor in an amount sufficient to temporarily delay the production of said signal.
Claim 5
Dependent Claim
20 words
The method of claim 3 wherein said signal producing system members are an enzyme and a substrate for said enzyme.
Claim 6
Dependent Claim
11 words
The method of claim 4 wherein said enzyme is a peroxidase.
Claim 7
Dependent Claim
17 words
The method of claim 4 wherein said exhaustible signal inhibitor is an alternative substrate for said enzyme.
Claim 8
Dependent Claim
38 words
The method of claim 4 wherein said delay step comprises the reaction of said exhaustible signal inhibitor with a product of the reaction of said enzyme with its substrate, said product being capable of generating a detectable signal.
Claim 9
Dependent Claim
19 words
The method of claim 4 wherein said exhaustible signal inhibitor is ascorbic acid or a salt or ester thereof.
Claim 10
Dependent Claim
54 words
The method of claim 3 wherein said exhaustible signal inhibitor is present in an amount sufficient to delay the production of said signal for a period of time approximately equivalent to the period of time for one member of said specific binding pair to bind with the other member of said specific binding pair.
References Cited (Backward Citations)
US3413197A
Cited
US3862885A
Cited
US4281065A
Cited
US4234680A
Cited
EP0058428A2
Cited
EP0100619A1
Cited
US4435504A
Cited
EP0103958A1
Cited
US4444880A
Cited
EP0123902A2
Cited
US4837395A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-02-17 | 1995-08-19 | 1996-02-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-02-17 | 1999-08-19 | 2000-02-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-02-17 | 2003-08-19 | 2004-02-18 | $7700.00 | Window Closed / Lapsed |