EP0103958A1
Expired / Lapsed
EP
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Immunoassay method
Assignee: Syva Co
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Filing: 1983-07-26
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Granted: —
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Expires: 2003-07-26
Abstract
Abstract In a solid support "dip-stick" immunoassay wherein a peroxidase catalyses the formation of a dye, ascorbate interference with the assay is reduced or eliminated by impregnating the support with periodate.
Inventors
- 👤 Henry Tom
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-04 17:04
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/28; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54393; G01N33/544; G01N33/548; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S435/962; Y10S435/963; Y10S435/964; Y10S435/97; Y10S436/00; Y10S436/807; Y10S436/81; Y10S436/825; Y10T; Y10T436/00; Y10T436/25; Y10T436/25125; Y10T436/2525
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
99 words
1 . An immunoassay method employing a bibulous su p- port to which is attached a mip and the amount of a mipperoxidase conjugate which binds to the mip on the support is related to the amount of analyte in a sample, where the peroxidase catalyzes the formation of a dye which binds to said support and ascorbate in said sample interferes with the production of said dye, characterised in that said support is impregnated with a sufficient amount of periodate to reduce said ascorbate interference to a level which does not interfere with the detection of said analyte.
Claim 2
Dependent Claim
99 words
1 . An immunoassay method employing a bibulous su p- port to which is attached a mip and the amount of a mipperoxidase conjugate which binds to the mip on the support is related to the amount of analyte in a sample, where the peroxidase catalyzes the formation of a dye which binds to said support and ascorbate in said sample interferes with the production of said dye, characterised in that said support is impregnated with a sufficient amount of periodate to reduce said ascorbate interference to a level which does not interfere with the detection of said analyte.
Claim 3
Dependent Claim
21 words
A method according to Claim 1, wherein said periodate is impregnated in combination with a protein stabilizer and a saccharide stabilizer.
Claim 4
Dependent Claim
18 words
A method according to Claim 2, wherein said protein stabilizer is albumin and said saccharide stabilizer is sucrose.
Claim 5
Dependent Claim
22 words
A method according to Claim 1, wherein an oxidase capable of catalyzing the formation of hydrogen peroxide is bound to said support.
Claim 6
Independent Claim
18 words
A method according to any of Claims 1 or 4, wherein an antibody is bound to said support.
References Cited (Backward Citations)
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