EP0058428A2
Expired / Lapsed
EP
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An enzyme immuno-assay for simultaneously measuring a plurality of samples and test vessel for carrying out this method
Assignee: Eisai Co Ltd
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Filing: 1982-02-16
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Granted: —
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Expires: 2002-02-16
Abstract
Abstract A method for measuring substances by enzyme immunoassay and a test vessel for carrying out this method are described. The method according to the invention relates to an improvement in the sandwich method for enzyme immunoassay. In controlling the temperature conditions the fluctiation of results can be minimized.
Inventors
- 👤 Yuzaburo Namba
- 👤 Tohru Naraki
- 👤 Takashi Sawada
- 👤 Toyoshiro Kitamura
- 👤 Minoru Tohda
- 👤 Tomiaki Morimoto
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-04 12:21
CPC Classifications
B; B01; B01L; B01L3/00; B01L3/50; B01L3/508; B01L3/5085; B01L3/50851; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
202 words
A method for measuring substances by enzyme immune- assay , characterized by simultaneously measuring a plurality of specimens by said immunoassay which comprises the steps of: a. reacting a substance to be measured (specimens) with a material which will be specifically combined with the substance to be measured and which has been made in a form of the solid phase; b. separating the reaction mixture in the Step (a) into the solid phase and the liquid phase; c. reacting the solid phase with an enzyme-labelled product of a material which will be specifically combined with the substance to be measured; d. separating the reaction mixture in the Step (c) into the solid phase and the liquid phase; e. reacting the solid phase or the liquid phase with a substrate for the enzyme in the Step (c); and f. measuring the optical density of the reaction mixture; in Step (a), successively contacting the respective specimens with the material which will be specifically combined with the substance to be measured and which has been made in the solid phase, under cooling condition at a temperature ranging from 0°C. to 15°C., followed by simultaneously incubating them at a temperature ranging from room temperature to 45°C.
Claim 2
Independent Claim
202 words
A method for measuring substances by enzyme immune- assay , characterized by simultaneously measuring a plurality of specimens by said immunoassay which comprises the steps of: a. reacting a substance to be measured (specimens) with a material which will be specifically combined with the substance to be measured and which has been made in a form of the solid phase; b. separating the reaction mixture in the Step (a) into the solid phase and the liquid phase; c. reacting the solid phase with an enzyme-labelled product of a material which will be specifically combined with the substance to be measured; d. separating the reaction mixture in the Step (c) into the solid phase and the liquid phase; e. reacting the solid phase or the liquid phase with a substrate for the enzyme in the Step (c); and f. measuring the optical density of the reaction mixture; in Step (a), successively contacting the respective specimens with the material which will be specifically combined with the substance to be measured and which has been made in the solid phase, under cooling condition at a temperature ranging from 0°C. to 15°C., followed by simultaneously incubating them at a temperature ranging from room temperature to 45°C.
Claim 3
Dependent Claim
32 words
The method for measuring a substance according to Claim 1 wherein the substance to be measured is a material which has a combination partner which will be specifically combined with said substance.
Claim 4
Dependent Claim
38 words
The method for measuring a substance according to Claim 1 wherein the material which will be specifically combined with the substance to be measured is made in the' solid phase by fixing said substance to an insoluble carrier.
Claim 5
Dependent Claim
51 words
The method for measuring a substance according to Claim 1 wherein the successive addition of the enzyme-labelled product to the solid phase in the Step c is effected at a temperature ranging from 0°C. to 15°C., and the reaction is then conducted at a temperature ranging from room temperature to 450C.
Claim 6
Dependent Claim
57 words
A test vessel which comprises closely contacting and unifying, to a receiver made of a synthetic resin providing at least one concave portion, a base frame consisting of a thin layer plate or a foil which comprises a metal as a predominant component, the metal being capable of closely contacting to the outer wall of the receiver.
Claim 7
Dependent Claim
57 words
A test vessel which comprises closely contacting and unifying, to a receiver made of a synthetic resin providing at least one concave portion, a base frame consisting of a thin layer plate or a foil which comprises a metal as a predominant component, the metal being capable of closely contacting to the outer wall of the receiver.
Claim 8
Dependent Claim
19 words
The test vessel according to Claim 5, wherein the vessel is used for simultaneously measuring specimens by enzyme immunoassay.
Claim 9
Dependent Claim
19 words
The test vessel according to Claim 5, wherein the vessel is used for the method according to Claim 1.
References Cited (Backward Citations)
No citation records indexed.
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