Patent US5086476A successfully refreshed from Google Patents.
US5086476A
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Method and apparatus for determining a proliferation index of a cell sample
Assignee: Cell Analysis Systems Inc
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Filing: 1989-02-24
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Granted: 1992-02-04
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Expires: 2009-02-24
Abstract
Abstract An image processing method and apparatus determines a proliferation index of a cell sample by staining the cells with a chromogen for a proliferation substance and a counterstain for the cell nuclei. The chromogen is activated by an antibody-enzyme conjugate which binds to the proliferation substance to produce a stained cell sample. The stained cell sample is examined with an optical microscope, forming a portion of the apparatus, which produces a magnified cell sample image. The apparatus optically filters the cell sample image and produces a pair of optically enhanced proliferation substance and cell nuclei images. The enhanced images are electronically analyzed to determine the amounts of cell nuclei and proliferation substance appearing in the images, respectively. The amounts are then compared to yield a proliferation index for the portion of the cell sample appearing in the cell sample image.
Inventors
- 👤 James W. Bacus
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 05:00
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/569; G01N33/56966; G01N15/00; G01N15/10; G01N15/14; G01N15/1468; G01N33/56972; G06; G06V; G06V20/00; G06V20/60; G06V20/69; G01N15/1429; G01N15/1433; G01N21/00; G01N21/62; G01N21/63; G01N21/64; G01N21/645; G01N21/6456; G01N21/6458; G01N2201/00; G01N2201/12; G01N2201/127
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
250 words
An apparatus for determining a proliferation index of a cell sample, comprising: first means for optically sensing portions of a cell sample having a proliferation optically enhanced substance thereon and producing a proliferation substance signal corresponding thereto wherein the first means further comprises an image enhancing optical filter allowing transmission of light for an optical absorbing region of a chromogen associated with the optically-enhanced proliferation substance at a reduced optical absorbing transmission region of a stain optically marking the cell nuclei; second means for optically sensing portions of a cell sample having optically marked cell nuclei and producing a cell nuclei signal corresponding thereto wherein the second means further comprises an optical filter allowing transmission of light at an optical absorbing region of a stain, which is optically marking the cell nuclei, and at an optical absorbing region of a chromogen associated with the optically-enhanced proliferation substance; first determining means coupled to the first sensing means for determining an amount of the proliferation substance and producing a proliferation substance amount signal corresponding thereto; second determining means coupled to the second sensing means for determining an amount of optically marked cell nuclei and producing a cell nuclei amount signal corresponding thereto; and proliferation index determining means coupled to the first determining means and receiving the proliferation substance amount signal and coupled to the second determining means and receiving the cell nuclei amount signal therefrom, for determining a proliferation index from the proliferation substance amount signal and the cell nuclei amount signal.
Claim 2
Independent Claim
250 words
An apparatus for determining a proliferation index of a cell sample, comprising: first means for optically sensing portions of a cell sample having a proliferation optically enhanced substance thereon and producing a proliferation substance signal corresponding thereto wherein the first means further comprises an image enhancing optical filter allowing transmission of light for an optical absorbing region of a chromogen associated with the optically-enhanced proliferation substance at a reduced optical absorbing transmission region of a stain optically marking the cell nuclei; second means for optically sensing portions of a cell sample having optically marked cell nuclei and producing a cell nuclei signal corresponding thereto wherein the second means further comprises an optical filter allowing transmission of light at an optical absorbing region of a stain, which is optically marking the cell nuclei, and at an optical absorbing region of a chromogen associated with the optically-enhanced proliferation substance; first determining means coupled to the first sensing means for determining an amount of the proliferation substance and producing a proliferation substance amount signal corresponding thereto; second determining means coupled to the second sensing means for determining an amount of optically marked cell nuclei and producing a cell nuclei amount signal corresponding thereto; and proliferation index determining means coupled to the first determining means and receiving the proliferation substance amount signal and coupled to the second determining means and receiving the cell nuclei amount signal therefrom, for determining a proliferation index from the proliferation substance amount signal and the cell nuclei amount signal.
Claim 3
Dependent Claim
63 words
An apparatus for determining a proliferation index of a cell sample as defined in claim 1, wherein the first means further comprises an image enhancing optical filter which allows transmission of light at an optical absorbing region of a chromogen associated with the proliferation substance and at an essentially one hundred percent optical transmission region of a stain optically marking the cell nuclei.
Claim 4
Dependent Claim
33 words
An apparatus for determining a proliferation index of a cell sample as defined in claim 2, wherein the first means further comprises means for storing a digitized image of the optically-enhanced proliferatino substance.
Claim 5
Dependent Claim
54 words
An apparatus for determining a proliferation index of a cell sample as defined in claim 1, wherein the first determining means further comprises means for determining an image area occupied by the optically-enhanced proliferation substance and wherein the proliferation substance amount signal is indicative of the image area occupied by the optically-enhanced proliferation substance.
Claim 6
Dependent Claim
55 words
An apparatus for determining a proliferation index of a cell sample as defined in claim 4, wherein the second determining means further comprises means for determining an image area occupied by the optically marked cell nuclei and the cell nuclei amount signal is indicative of the image area occupied by the optically marked cell nuclei.
Claim 7
Dependent Claim
62 words
An apparatus for determining a proliferation index of a cell sample as defined in claim 1, wherein the first determining means further comprises means for determining a number of cell nuclei in an image field having the optically-enhanced proliferation substance therein, and the proliferation substance amount signal is indicative of the number of optically marked cell nuclei having the optically-enhanced proliferation substance.
Claim 8
Dependent Claim
55 words
An apparatus for determining a proliferation index of a cell sample as defined in claim 6, wherein the second determining means further comprises means for determining a number of optically marked cell nuclei in the image field, and the cell nuclei amount signal is indicative of the optically marked cell nuclei in the image field.
References Cited (Backward Citations)
US3297879A
Cited
US3481659A
Cited
US3532412A
Cited
US3773425A
Cited
US3847486A
Cited
US4207554A
Cited
US4125828A
Cited
US3851156A
Cited
US3907437A
Cited
US3895854A
Cited
US3977791A
Cited
US4045772A
Cited
US4174178A
Cited
US4061914A
Cited
US4017192A
Cited
US4000417A
Cited
US4152410A
Cited
US4048616A
Cited
US4132767A
Cited
US4160817A
Cited
US4129854A
Cited
US4199748A
Cited
US4097845A
Cited
US4307376A
Cited
US4232970A
Cited
US4175860A
Cited
US4231660A
Cited
US4213036A
Cited
US4232001A
Cited
US4227814A
Cited
US4523278A
Cited
US4219440A
Cited
US4404683A
Cited
US4362386A
Cited
US4257709A
Cited
US4446871A
Cited
US4453266A
Cited
US4389669A
Cited
US4408231A
Cited
US4562593A
Cited
US4513438A
Cited
JPS5988716A
Cited
US4592089A
Cited
US4741043A
Cited
US4741043B1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-02-03 | 1995-08-05 | 1996-02-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-02-03 | 1999-08-05 | 2000-02-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-02-03 | 2003-08-05 | 2004-02-04 | $7700.00 | Window Closed / Lapsed |