US5084384A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Secretion of insulin-like growth factor-I
Assignee: Monsanto Technology LLC
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Filing: 1990-05-10
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Granted: 1992-01-28
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Expires: 2010-05-10
Abstract
Abstract A method for bacterially producing IGF-I is disclosed in which Gram-negative bacteria are caused to express a gene consisting of a lamB or ompF signal sequence operatively joined to a DNA sequence encoding IGF-I and producing IGF-I which is secreted into the periplasmic space of the bacteria.
Inventors
- 👤 Edith Wong
- 👤 Michael L. Bittner
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 13:00
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/70; C07; C07K; C07K14/00; C07K14/195; C07K14/24; C07K14/435; C07K14/575; C07K14/65; C07K2319/00; C07K2319/01; C07K2319/035; C07K2319/70; C07K2319/74; C07K2319/75; Y; Y10; Y10S; Y10S530/00; Y10S530/808
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
91 words
A method for producing mature IGF-I which comprises: causing expression of a gene in E. coli, said gene comprising, sequentially, a DNA sequence encoding a promoter operable in E. coli, a ribosome binding site, a DNA sequence encoding a lamB signal sequence "consisting of Met-Met-Ile-Thr-Leu-Arg-Lys-Leu-Pro-Leu-Ala-Val-Ala-Val-Ala-Ala-Gly-Val-Met-Ser-Ala-Gln-Ala-Met-Ala which is" directly joined to a DNA sequence encoding IGF-I "wherein said lamB signal sequence is free of any amino acids from the mature lamB protein," and a transcription termination sequence, and recovering the IGF-I which is secreted into the periplasmic space of the E. coli.
Claim 2
Independent Claim
91 words
A method for producing mature IGF-I which comprises: causing expression of a gene in E. coli, said gene comprising, sequentially, a DNA sequence encoding a promoter operable in E. coli, a ribosome binding site, a DNA sequence encoding a lamB signal sequence "consisting of Met-Met-Ile-Thr-Leu-Arg-Lys-Leu-Pro-Leu-Ala-Val-Ala-Val-Ala-Ala-Gly-Val-Met-Ser-Ala-Gln-Ala-Met-Ala which is" directly joined to a DNA sequence encoding IGF-I "wherein said lamB signal sequence is free of any amino acids from the mature lamB protein," and a transcription termination sequence, and recovering the IGF-I which is secreted into the periplasmic space of the E. coli.
Claim 3
Dependent Claim
23 words
The method of claim 1 wherein the E. coli is an E. coli having all the identifying characteristics of ATCC accession number 53588.
Claim 4
Dependent Claim
16 words
The method of claim 1 wherein the DNA sequence encoding IGF-I comprises E. coli preferred codons.
Claim 5
Dependent Claim
12 words
The method of claim 1 wherein the promoter is a recA promoter.
Claim 6
Dependent Claim
14 words
The method of claim 1 wherein the IGF-I is also secreted into the medium.
Claim 7
Dependent Claim
15 words
The method of claim 1 wherein the gene is carried on an extrachromosomal DNA molecule.
Claim 8
Dependent Claim
14 words
The method of claim 6 wherein the extrachromosomal DNA molecule is a plasmid DNA.
Claim 9
Dependent Claim
11 words
The method of claim 7 wherein the plasmid DNA is pMON2690.
Claim 10
Dependent Claim
14 words
The method of claim 6 wherein the extrachromosomal DNA also carries a selectable marker.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-01-27 | 1995-07-29 | 1996-01-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-01-27 | 1999-07-29 | 2000-01-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-01-27 | 2003-07-29 | 2004-01-28 | $7700.00 | Window Closed / Lapsed |