US4336336A
Expired / Lapsed
US
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Fused gene and method of making and using same
Assignee: Harvard University
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Filing: 1979-01-12
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Granted: 1982-06-22
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Expires: 1999-01-12
Abstract
Abstract This invention is a method for fusing, within a bacterial host, a gene for a cytoplasmic protein to a gene for a non-cytoplasmic protein, so that the hybrid protein produced is transported to, near, or beyond the cell surface for ease of collection and purification, or for immunological use. The invention also encompasses the fused gene created by the disclosed method.
Inventors
- 👤 Thomas J. Silhavy
- 👤 Howard A. Shuman
- 👤 Jon Beckwith
- 👤 Maxime Schwartz
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 03:47
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/11; C12N15/62
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
70 words
A fused gene which comprises a bacterial gene coding for a cytoplasmic protein fused to the non-cytoplasmic directing region of a gene coding for a non-cytoplasmic protein which is normally transported to or beyond the cell surface, such fused gene being capable of coding for a hybrid protein which is transported beyond the periplasmic space and either to the cell surface, or beyond the cell surface, of a bacterial host.
Claim 2
Dependent Claim
70 words
A fused gene which comprises a bacterial gene coding for a cytoplasmic protein fused to the non-cytoplasmic directing region of a gene coding for a non-cytoplasmic protein which is normally transported to or beyond the cell surface, such fused gene being capable of coding for a hybrid protein which is transported beyond the periplasmic space and either to the cell surface, or beyond the cell surface, of a bacterial host.
Claim 3
Dependent Claim
21 words
The fused gene of claim 1, further comprising a gene coding for a eukaryotic cell protein inserted into said fused gene.
Claim 4
Dependent Claim
20 words
The fused gene of claim 1, further comprising a gene coding for a viral protein inserted into said fused gene.
Claim 5
Dependent Claim
20 words
The fused gene of claim 1, further comprising a gene coding for a bacterial protein inserted into said fused gene.
Claim 6
Dependent Claim
44 words
A method for making the fused gene of claim 1 which comprises, within a host bacterium, fusing to a gene coding for said cytoplasmic protein the non-cytoplasmic-directing region of a gene coding for a non-cytoplasmic protein, and selecting the bacteria containing the fused gene.
Claim 7
Dependent Claim
26 words
The method as claimed in claim 5, which further comprises the step of inserting into said fused gene the gene coding for a eukaryotic cell protein.
Claim 8
Dependent Claim
25 words
The method as claimed in claim 5, which further comprises the step of inserting into said fused gene the gene coding for a viral protein.
Claim 9
Dependent Claim
25 words
The method as claimed in claim 5, which further comprises the step of inserting into said fused gene the gene coding for a bacterial protein.
Claim 10
Independent Claim
23 words
The method as claimed in any of claims 5 to 8 in which said host bacterium is any of the gram negative bacteria.
Claim 11
Independent Claim
23 words
The method as claimed in any of claims 5 to 8 in which said host bacterium is any of the gram positive bacteria.
Claim 12
Independent Claim
37 words
The method as claimed in any of claims 5 to 8 in which said gene coding for said cytoplasmic protein is part of a phage which is inserted into the host bacterial chromosome to create the fusion.
Claim 13
Independent Claim
24 words
The method as claimed in any of claims 5 to 8 in which said gene for said cytoplasmic protein is the gene for β-galactosidase.
Claim 14
Dependent Claim
20 words
The method as claimed in claim 9 in which said gene for said cytoplasmic protein is the gene for β-galactosidase.
Claim 15
Dependent Claim
20 words
The method as claimed in claim 10 in which said gene for said cytoplasmic protein is the gene for β-galactosidase.
Claim 16
Independent Claim
25 words
The method as claimed in any of claims 5 to 8 in which said gene coding for said non-cytoplasmic protein is the gene for λ-receptor.
Claim 17
Dependent Claim
15 words
The method as claimed in claim 11 in which said phage is a lambdoid phage.
Claim 18
Dependent Claim
16 words
The method as claimed in claim 16 in which said lambdoid phage is λ P1 (209,118).
Claim 19
Dependent Claim
20 words
The method as claimed in claim 11 in which said gene for said cytoplasmic protein is the gene for β-galactosidase.
Claim 20
Dependent Claim
22 words
The method as claimed in claim 16 or 17 in which said gene for said cytoplasmic protein is the gene for β-galactosidase.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-06-21 | 1985-12-21 | 1986-06-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-06-21 | 1989-12-21 | 1990-06-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-06-21 | 1993-12-21 | 1994-06-22 | $7700.00 | Window Closed / Lapsed |