EP0105521A2
Expired / Lapsed
EP
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Novel DNA, microorganism transformed therewith and use thereof
Assignee: Takeda Pharmaceutical Co Ltd
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Filing: 1983-10-04
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Granted: —
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Expires: 2003-10-04
Abstract
Abstract Peptide can be obtained, not in the form of a chimera peptide with rec A protein, by growing a transformant which contains a DNA containing one or more rec A promoters, at the 3'-end of the rec A promoter or promoters no translational start codon ATG being directly linked, and containing downstream from the rec A promoter or promoters a peptide-encoding nucleotide beginning with a translational start codon and recovering the peptide.
Inventors
- 👤 Koichi Igarashi
- 👤 Tsutomu Kurokawa
- 👤 Reiko Sasada
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-08 20:39
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/70; C07; C07K; C07K16/00
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
28 words
A DNA which contains one or more rec A promoters, at the 3'-end of the rec A promoter or promoters no translational start codon ATG being directly linked.
Claim 2
Dependent Claim
28 words
A DNA which contains one or more rec A promoters, at the 3'-end of the rec A promoter or promoters no translational start codon ATG being directly linked.
Claim 3
Dependent Claim
31 words
A DNA according to Claim 1, wherein a nucleotide or nucleotides possessing a restriction enzyme recognition site are linked at one or each end of the rec A promoter or promoters.
Claim 4
Dependent Claim
20 words
A DNA according to Claim 1, wherein the number of the rec A promoters contained therein is one or two.
Claim 5
Dependent Claim
23 words
A DNA according to Claim 1, wherein the rec A promoter is the nucleotide of the nucleotide sequence as shown in Figure 1.
Claim 6
Dependent Claim
31 words
A DNA according to Claim 1, which contains downstream from therec A promoter or promoters a peptide-encoding nucleotide beginning with a translational start codon other than the rec A structural gene.
Claim 7
Dependent Claim
13 words
A DNA according to Claim 5, wherein the translational start codon is ATG.
Claim 8
Dependent Claim
24 words
A DNA according to Claim 5, wherein the peptide-encoding nucleotide codes for a peptide containing human immunoglobulin E H-chain polypeptide or a fragment thereof.
Claim 9
Dependent Claim
31 words
A transformant which contains a DNA containing one or more rec A promoters, at the 3'-end of the rec A promoter or promoters no translational start codon ATG being directly linked.
Claim 10
Dependent Claim
31 words
A transformant which contains a DNA containing one or more rec A promoters, at the 3'-end of the rec A promoter or promoters no translational start codon ATG being directly linked.
Claim 11
Dependent Claim
34 words
A transformant according to Claim 8, wherein the DNA contains downstream from the rec A promoter or promoters a peptide-encoding nucleotide beginning with a translational start codon other than the rec A structural gene.
Claim 12
Dependent Claim
10 words
A transformant according to Claim 8, which is Escherichia coli.
Claim 13
Dependent Claim
12 words
The transformant according to Claim 8, which is Escherichia coli 294/pGETrec301 (IFO-14287).
Claim 14
Independent Claim
83 words
A method of producing a peptide, which comprises al growing a transformant which contains a DNA containing one or more rec A promoters, at the 3'-end of the rec A promoter or promoters no translational start codon ATG being directly linked, and containing downstream from the rec A promoter or promoters a peptide encoding nucleotide beginning with a translational start codon other than the rec A structural gene, bl accumulating said peptide in the culture, and c) isolating the same from said culture.
Claim 15
Independent Claim
83 words
A method of producing a peptide, which comprises al growing a transformant which contains a DNA containing one or more rec A promoters, at the 3'-end of the rec A promoter or promoters no translational start codon ATG being directly linked, and containing downstream from the rec A promoter or promoters a peptide encoding nucleotide beginning with a translational start codon other than the rec A structural gene, bl accumulating said peptide in the culture, and c) isolating the same from said culture.
Claim 16
Dependent Claim
22 words
A method according to Claim 12, wherein the peptide is a peptide containing human immunoglobulin E H-chain polypeptide or a fragment thereof.
Claim 17
Independent Claim
213 words
A method of producing a DNA containing one or more rec A promoters, at the 3 1- end of the rec A promoter or promoters no translational start codon ATG being directly linked, which comprises al digesting a DNA containing the rec A promoter and the rec A structural gene or a fragment thereof with a restriction enzyme to give a nucleotide containing a fragment of the rec A promoter of which the SD sequence is missing, and b) ligating the thus obtained nucleotide with chemically synthesized nucleotide or nucleotides containing the SD sequence to give a DNA containing the rec A promoter, at the 3 1- end of the rec A promoter or promoters no translational start codon ATG being directly linked, and if desired, cl ligating the DNA with a nucleotide or nucleotides possessing a restriction enzyme recognition site, and if desired, d) inserting the DNA into a plasmid to give a recombinant plasmid containing one or more rec A promoters, at the 3'-end of the rec A promoter or promoters, and if desired, el transforming a host organism with said recombinant plasmid, and if desired, f) inserting a peptide-encoding nucleotide having a translational start codon at its 5'-end downstream from the rec A promoter or promoters in said recombinant plasmid.
Claim 18
Independent Claim
213 words
A method of producing a DNA containing one or more rec A promoters, at the 3 1- end of the rec A promoter or promoters no translational start codon ATG being directly linked, which comprises al digesting a DNA containing the rec A promoter and the rec A structural gene or a fragment thereof with a restriction enzyme to give a nucleotide containing a fragment of the rec A promoter of which the SD sequence is missing, and b) ligating the thus obtained nucleotide with chemically synthesized nucleotide or nucleotides containing the SD sequence to give a DNA containing the rec A promoter, at the 3 1- end of the rec A promoter or promoters no translational start codon ATG being directly linked, and if desired, cl ligating the DNA with a nucleotide or nucleotides possessing a restriction enzyme recognition site, and if desired, d) inserting the DNA into a plasmid to give a recombinant plasmid containing one or more rec A promoters, at the 3'-end of the rec A promoter or promoters, and if desired, el transforming a host organism with said recombinant plasmid, and if desired, f) inserting a peptide-encoding nucleotide having a translational start codon at its 5'-end downstream from the rec A promoter or promoters in said recombinant plasmid.
Claim 19
Independent Claim
44 words
A method of preparing a transformant which contains a DNA containing one or more rec A promoters, at the 3'-end of the rec A promoter or promoters no translational start codon ATG being directly linked, which comprises transforming a host organism with said DNA.
Claim 20
Independent Claim
44 words
A method of preparing a transformant which contains a DNA containing one or more rec A promoters, at the 3'-end of the rec A promoter or promoters no translational start codon ATG being directly linked, which comprises transforming a host organism with said DNA.
References Cited (Backward Citations)
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