US5082774A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Recombinant human nerve growth factor
Assignee: General Hospital Corp
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Filing: 1988-08-30
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Granted: 1992-01-21
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Expires: 2008-08-30
Abstract
Abstract An expression vector replicable in a mammalian cell includes a prepro-NGF encoding DNA sequence operably linked with regulatory DNA capable of effecting the expression of the prepro-NGF encoding DNA sequence in the cell. Also, a mammalian cell is transfected with such an expression vector. Also, a method for making recombinant human nerve growth factor includes transfecting a mammalian cell with such an expression vector, maintaining the cell in a culture medium, and isolating recombinant human nerve growth factor from the medium. Also, a composition includes recombinant human nerve growth factor made according to such a method combined with a pharmaceutically acceptable carrier.
Inventors
- 👤 Gerhard Heinrich
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 01:19
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85; C07; C07K; C07K14/00; C07K14/435; C07K14/475; C07K14/48; A; A61; A61K; A61K38/00
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
66 words
A vector capable of replicating in a mammalian cell, said vector comprising a human prepro-NGF encoding DNA sequence consisting essentially of the coding region of exon 4 of the human NGF gene and regulator DNA operationally associated with and capable of effecting the expression of said human prepro-NGF encoding DNA sequence, said vector being capable of expressing biologically active recombinant human NGF in said mammalian cell.
Claim 2
Dependent Claim
66 words
A vector capable of replicating in a mammalian cell, said vector comprising a human prepro-NGF encoding DNA sequence consisting essentially of the coding region of exon 4 of the human NGF gene and regulator DNA operationally associated with and capable of effecting the expression of said human prepro-NGF encoding DNA sequence, said vector being capable of expressing biologically active recombinant human NGF in said mammalian cell.
Claim 3
Dependent Claim
11 words
A mammalian cell transfected with the expression vector of claim 1.
Claim 4
Dependent Claim
16 words
The mammalian cell of claim 2 wherein said cell is transiently transfected with said expression vector.
Claim 5
Dependent Claim
16 words
The mammalian cell of claim 3 wherein said cell is stably transfected with said expression vector.
Claim 6
Dependent Claim
2 words
Plasmid VP2.
Claim 7
Dependent Claim
2 words
Plasmid VP2.
Claim 8
Dependent Claim
10 words
A mammalian cell transfected with the plasmid of claim 5.
Claim 9
Dependent Claim
11 words
The cell of claim 6, said cell being a COS cell.
Claim 10
Dependent Claim
11 words
The cell of claim 6, said cell being a CHO cell.
Claim 11
Dependent Claim
39 words
A method for making recombinant human nerve growth factor, comprising transfecting a mammalian cell with the expression vector of claim 1, maintaining said mammalian cell in a culture medium, and isolating recombinant human nerve growth factor from said medium.
References Cited (Backward Citations)
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Cited
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Cited
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Cited
US4770999A
Cited
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Cited
WO1988000975A1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-01-20 | 1995-07-22 | 1996-01-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-01-20 | 1999-07-22 | 2000-01-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-01-20 | 2003-07-22 | 2004-01-21 | $7700.00 | Window Closed / Lapsed |