US4810643A
Expired / Lapsed
US
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Production of pluripotent granulocyte colony-stimulating factor
Assignee: Amgen Inc
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Filing: 1986-03-03
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Granted: 1989-03-07
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Expires: 2006-03-03
Abstract
Abstract Disclosed are novel polypeptides possessing part or all of the primary structural conformation and one or more of the biological properties of a mammalian (e.g., human) pluripotent granulocyte colony-stimulating factor ("hpG-CSF") which are characterized in preferred forms by being the product of procaryotic or eucaryotic host expression of an exogenous DNA sequence. Sequences coding for part or all of the sequence of amino acid residues of hpG-CSF or for analogs thereof may be incorporated into autonomously replicating plasmid or viral vectors employed to transform or transfect suitable procaryotic or eucaryotic host cells such as bacteria, yeast or vertebrate cells in culture. Products of expression of the DNA sequences display, i.e., the physical and immunological properties and in vitro biological activities of isolates of hpG-CSF derived from natural sources. Disclosed also are chemically synthesized polypeptides sharing the biochemical and immunological properties of hpG-CSF.
Inventors
- 👤 Lawrence M. Souza
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 12:43
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/18; C07K16/24; C07K16/243; C12; C12N; C12N15/00; A; A61; A61P; A61P17/00; A61P17/02; A61P25/00; A61P25/04; A61P29/00; A61P35/00; A61P35/02; A61P43/00; A61P7/00; A61P7/06; C07K14/00; C07K14/435; C07K14/52; C07K14/53; C07K14/535; C07K7/00; A61K; A61K38/00
Claims Analysis
Total Claims: 33
Claim 1
Independent Claim
102 words
A purified and isolated DNA molecule for use in securing expression in a procaryotic or eucaryotic host cell of a polypeptide product having at least a part of the primary structural conformation and the hematopoietic biological activity of naturally-occurring pluripotent granulocyte colony-stimulting factor, said DNA selected from among: (a) the DNA molecules set out in FIGS. 2 and 3 or their complementary strands; (b) DNA molecules which hybridize to the DNA molecules defined in (a) or fragments thereof; and (c) DNA molecules which, but for the degeneracy of the genetic code, would hybridize to the DNA molecules defined in (a) and (b).
Claim 2
Dependent Claim
102 words
A purified and isolated DNA molecule for use in securing expression in a procaryotic or eucaryotic host cell of a polypeptide product having at least a part of the primary structural conformation and the hematopoietic biological activity of naturally-occurring pluripotent granulocyte colony-stimulting factor, said DNA selected from among: (a) the DNA molecules set out in FIGS. 2 and 3 or their complementary strands; (b) DNA molecules which hybridize to the DNA molecules defined in (a) or fragments thereof; and (c) DNA molecules which, but for the degeneracy of the genetic code, would hybridize to the DNA molecules defined in (a) and (b).
Claim 3
Dependent Claim
37 words
A purified and isolated DNA molecule coding for procaryotic or eucaryotic host expression of a polypeptide product having part or all of the primary structural conformation and the hematopoietic biological activity of human pluripotent granulocyte colony-stimulating factor.
Claim 4
Dependent Claim
37 words
A purified and isolated DNA molecule coding for procaryotic or eucaryotic host expression of a polypeptide product having part or all of the primary structural conformation and the hematopoietic biological activity of human pluripotent granulocyte colony-stimulating factor.
Claim 5
Dependent Claim
7 words
A cDNA molecule according to claim 2.
Claim 6
Dependent Claim
8 words
A genomic DNA molecule according to claim 3.
Claim 7
Dependent Claim
8 words
A maufactured DNA molecule according to claim 2.
Claim 8
Dependent Claim
21 words
A manufactured DNA molecule according to claim 5 and including one or more codons preferred for expression in E. coli cells.
Claim 9
Dependent Claim
20 words
A manufactured DNA molecule according to claim 5 and including one or more codons preferred for expression in yeast cells.
Claim 10
Dependent Claim
14 words
A DNA molecule according to claim 2 covalently associated with a detectable label substance.
Claim 11
Dependent Claim
14 words
A DNA molecule according to claim 8 wherein the detectable label is a radiolabel.
Claim 12
Dependent Claim
8 words
A single-stranded DNA molecule according to claim 8.
Claim 13
Dependent Claim
27 words
A purified and isolated DNA molecule coding for a polypeptide fragment or polypeptide analog of naturally-occurring pluripotent granulocyte colony-stimulating factor and possessing the hematopoietic biological activity thereof.
Claim 14
Dependent Claim
27 words
A purified and isolated DNA molecule coding for a polypeptide fragment or polypeptide analog of naturally-occurring pluripotent granulocyte colony-stimulating factor and possessing the hematopoietic biological activity thereof.
Claim 15
Dependent Claim
16 words
A purified and isolated DNA molecule according to claim 11 coding for [Ala 1 ] hpG-CSF.
Claim 16
Dependent Claim
21 words
A biologically functional plasmid or viral DNA vector including a DNA molecule according to one of claims 1, 2 or 11.
Claim 17
Dependent Claim
29 words
A procaryotic or eucaryotic host cell stably transformed or transfected with a DNA vector according to claim 13 in a manner allowing expression of said polypeptide, fragment or analog.
Claim 18
Dependent Claim
78 words
A process for the production of a polypeptide product having part or all of the primary structural conformation and the hematopoietic biological activity of naturally occurring pluripotent granulocyte colony-stimulating factor, said process comprising: growing, under suitable nutrient conditions, procaryotic or eucaryotic host cells transformed or transfected with a DNA molecule according to claim 1, 2 or 11 in a manner allowing expression of said polypeptide product, and isolating desired polypeptide product of the expression of said DNA molecule.
Claim 19
Dependent Claim
15 words
A purified and isolated DNA molecule according to claim 11 coding for [Ser 17 ]hpG-CSF.
Claim 20
Dependent Claim
20 words
A polypeptide product of the expression in a procaryotic or eucaryotic host cell of a DNA according to claim 15.
Claim 21
Dependent Claim
16 words
A biologically functional plasmid or viral DNA vector including a DNA molecule according to claim 16.
Claim 22
Dependent Claim
18 words
A procaryotic or eucaryotic host cell stably transformed or transfected with a DNA vector according to claim 17.
Claim 23
Dependent Claim
68 words
A purified and isolated DNA molecule according to claim 11 coding for an analog of hpG-CSF selected from the group consisting of: [Met -1 ]hpG-CSF; [Ser 36 ]hpG-CSF; [Ser 42 ]hpG-CSF; [Ser 64 ]hpG-CSF; [Ser 74 ]hpG-CSF; [Met -1 , Ser 17 ]hpG-CSF; [Met -1 , Ser 36 ]hpG-CSF; [Met -1 , Ser 42 ]hpG-CSF; [Met -1 , Ser 64 ]hpG-CSF; and [Met -1 , Ser 74 ]hpG-CSF.
Claim 24
Dependent Claim
28 words
A procaryotic or eucaryotic cell transformed or transfected with a DNA molecule according to claim 1 in a manner allowing the host cell to express said polypeptide product.
Claim 25
Dependent Claim
9 words
An E. coli host cell according to claim 21.
Claim 26
Dependent Claim
8 words
A yeast host cell according to claim 21.
Claim 27
Dependent Claim
9 words
A mammalian host host cell according to claim 21.
Claim 28
Dependent Claim
9 words
An E. coli host cell according to claim 14.
Claim 29
Dependent Claim
8 words
A yeast host cell according to claim 14.
Claim 30
Dependent Claim
8 words
A mammalian host cell according to claim 14.
Claim 31
Dependent Claim
14 words
A process according to claim 15 wherein said host cells are E. coli cells.
Claim 32
Dependent Claim
13 words
A process according to claim 15 wherein said host cells are yeast cells.
Claim 33
Dependent Claim
13 words
A process according to claim 15 wherein said host cells are mammalian cells.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-03-06 | 1992-09-05 | 1993-03-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-03-06 | 1996-09-05 | 1997-03-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-03-06 | 2000-09-05 | 2001-03-07 | $7700.00 | Window Closed / Lapsed |