EP0121338B1
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Biosynthetically produced human nerve growth factor, process for producing it, compositions containing it, dna sequence encoding it, vectors containing the sequence and host cells transformed thereby
Assignee: Genentech Inc
•
Filing: 1984-03-02
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Granted: 1989-08-09
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Expires: 2004-03-02
Abstract
Abstract The beta -subunit of human nerve growth factor is prepared by recombinant DNA technology.
Inventors
- 👤 Alane Molly Gray
- 👤 Axel Ullrich
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-06 05:19
CPC Classifications
C; C12; C12N; C12N15/00; C07; C07K; C07K14/00; C07K14/435; C07K14/475; C07K14/48; A; A61; A61P; A61P25/00; A61P43/00; A61K; A61K38/00
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
175 words
A process which comprises expressing in a recombinant host cell DNA encoding a polypeptide of the amino acid sequence ser - ser - ser - his - pro - ile - phe - his - arg - gly - glu - phe - ser - val - cys - asp - ser - val - ser - val - trp - gly - asp - lys - thr - thr - ala - thr - asp - ile - lys - gly - glys - glu - val - met - val - leu - gly - glu - val- asn - ile - asn - asn - ser - val - phe - lys - gin - tyr - phe - phe - glu - thr - lys - cys - arg - asp - pro - asn -pro-val-asp-ser-gly-cys-arg-gly-ile-asp-ser-lys-his-trp-asn-ser-tyr-cys-thr-thr-thr-his- thr-phe-val-lys-ala-leu-thr-met-asp-gly-lys-gln-ala-ala-trp-arg-phe-ile-arg-ile-asp-thr- ala - cys - val - cys - val - cys - val - leu - ser - arg - lys - ala - val - arg and having the properties of human ß-NGF.
Claim 2
Dependent Claim
175 words
A process which comprises expressing in a recombinant host cell DNA encoding a polypeptide of the amino acid sequence ser - ser - ser - his - pro - ile - phe - his - arg - gly - glu - phe - ser - val - cys - asp - ser - val - ser - val - trp - gly - asp - lys - thr - thr - ala - thr - asp - ile - lys - gly - glys - glu - val - met - val - leu - gly - glu - val- asn - ile - asn - asn - ser - val - phe - lys - gin - tyr - phe - phe - glu - thr - lys - cys - arg - asp - pro - asn -pro-val-asp-ser-gly-cys-arg-gly-ile-asp-ser-lys-his-trp-asn-ser-tyr-cys-thr-thr-thr-his- thr-phe-val-lys-ala-leu-thr-met-asp-gly-lys-gln-ala-ala-trp-arg-phe-ile-arg-ile-asp-thr- ala - cys - val - cys - val - cys - val - leu - ser - arg - lys - ala - val - arg and having the properties of human ß-NGF.
Claim 3
Dependent Claim
22 words
A process according to claim 1 in which the initial serine residue in the stated sequence is preceded by the methionine residue.
Claim 4
Dependent Claim
29 words
A process which comprises expressing in a recombinant host cell DNA encoding a polypeptide of claim 1 or claim 2, and recovering the polypeptide from the host cell culture.
Claim 5
Dependent Claim
29 words
A process according claim 3 wherein the polypeptide is expressed in E. coli as a mature sequence free from additional protein, other than N-terminal methionyl, fused to the sequence.
Claim 6
Dependent Claim
33 words
A process which, following the preparation of a said polypeptide by the process of any of claims 1 to 4, comprises the use of said polypeptide in the production of a pharmaceutical preparation.
Claim 7
Dependent Claim
15 words
A process according to claim 5 in which the preparation is suitable for parenteral administration.
Claim 8
Dependent Claim
18 words
A process of claim 5 or claim 6 wherein the pharmaceutical preparation is for use in nerve treatment.
Claim 9
Dependent Claim
21 words
A process involving the preparation of a DNA isolate comprising a sequence encoding the polypeptide of claim 1 or claim 2.
Claim 10
Dependent Claim
34 words
A process involving the preparation of a replicable expression vector comprising a DNA sequence encoding the polypeptide of claim 1 or claim 2 for expression in a suitable host cell transformed with the vector.
Claim 11
Dependent Claim
29 words
A recombinant host cell transformed with the expression vector as defined in claim 9 so as to be capable of producing said polypeptide by expression of said coding sequence.
Claim 12
Dependent Claim
15 words
A recombinant host cell according to claim 10 which is a strain of E. coli.
References Cited (Backward Citations)
No citation records indexed.
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