US5077199A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Ready to use liquid reagent for determining the glucose content in blood
Assignee: Amenarini
•
Filing: 1985-01-03
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Granted: 1991-12-31
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Expires: 2005-01-03
Abstract
Abstract Stable and ready to use liquid reagent for determining the glucose content in blood, comprising a glucose-oxidase free from catalase and a nonionic surface-active agent, such as, for example, the polyoxyethylene derivatives, hydroxypolyethylene ethoxydodecane, the lauryl ether of polyoxyethylene-glycol, as a stabilizer.
Inventors
- 👤 Umberto Basagni
- 👤 Francesco Bonicolini
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 12:00
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/54; Y; Y10; Y10S; Y10S435/00; Y10S435/81
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
48 words
A ready-to-use aqueous liquid reagent for colorimetric determination of the glucose content in blood, comprising: (a) from 9,000 to 40,000 u.i/liter of enzymatically-active glucose oxidase free from catalase; and (b) from 5 mg/liter to 50 g/liter of a nonionic surface active agent effective to stabilize the liquid reagent.
Claim 2
Dependent Claim
48 words
A ready-to-use aqueous liquid reagent for colorimetric determination of the glucose content in blood, comprising: (a) from 9,000 to 40,000 u.i/liter of enzymatically-active glucose oxidase free from catalase; and (b) from 5 mg/liter to 50 g/liter of a nonionic surface active agent effective to stabilize the liquid reagent.
Claim 3
Dependent Claim
16 words
A liquid reagent according to claim 1, wherein the surface active agent is a polyoxyethylene derivative.
Claim 4
Dependent Claim
15 words
A liquid reagent according to claim 2, wherein the surface active agent is polyoxyethylene-lauryl alcohol.
Claim 5
Dependent Claim
14 words
A liquid reagent according to claim 2, wherein the surface active agent is polyoxyethylene-octylphenol.
Claim 6
Dependent Claim
31 words
A liquid reagent according to claim 1, further comprising peroxidase and a chromogenic system such that upon combination of the reagent with a glucose-containing sample a colored product will be formed.
Claim 7
Dependent Claim
19 words
A liquid reagent according to claim 5, wherein the chromogenic system comprises phenol and 4-aminophenazone or a derivative thereof.
Claim 8
Dependent Claim
15 words
A liquid reagent according to claim 5, wherein the chromogenic system comprises phenol and 4-aminoantipyrine.
References Cited (Backward Citations)
GB1592632A
Cited
US4340448A
Cited
EP0024578A1
Cited
EP0044432A2
Cited
US4540659A
Cited
WO1983003254A1
Cited
US4473638A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-12-30 | 1995-07-01 | 1995-12-31 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-12-30 | 1999-07-01 | 1999-12-31 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-12-30 | 2003-07-01 | 2003-12-31 | $7700.00 | Window Closed / Lapsed |