US4473638A
Expired / Lapsed
US
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Specific binding assay
Assignee: Dade Behring Inc
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Filing: 1982-04-22
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Granted: 1984-09-25
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Expires: 2002-04-22
Abstract
Abstract An improvement in a specific binding assay for determining a ligand in a liquid medium, in which assay the labelled conjugate includes FAD and the ligand and in which the labelled conjugate can bind to apoglucose oxidase to form a holoenzyme is provided. The improvement, which renders the assay practical at 37° C., comprises carrying out the assay in the presence of at least about a 250 mM concentration of a polyhydric aldehyde or alcohol, including substrate.
Inventors
- 👤 Karen Auditore-Hargreaves
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 01:17
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/535; G01N33/536; G01N33/542
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
149 words
In a specific binding assay for quantitatively detecting a ligand in a liquid sample, said assay comprising the steps of (1) reacting said liquid sample with a liquid reagent comprising (i) a ligand binding component, (ii) a conjugate comprising flavin adenine dinucleotide and a ligand, (iii) apoglucose oxidase and (iv) a substrate, wherein the conjugate is capable of binding to the apoglucose oxidase to form a holoenzyme which, in turn, is capable of catalyzing the conversion of the substrate into a detectable product; and (2) measuring the detectable product, the improvement comprising carrying out the assay in a liquid solution at about 37° C. in the presence of at least a 250 mM final concentration of a polyhydric aldehyde or polyhydric alcohol, the polyhydric aldehyde or polyhydric alcohol being the same as or different from the substrate, whereby the formation of the holoenzyme from the apoglucose oxidase is provided.
Claim 2
Dependent Claim
149 words
In a specific binding assay for quantitatively detecting a ligand in a liquid sample, said assay comprising the steps of (1) reacting said liquid sample with a liquid reagent comprising (i) a ligand binding component, (ii) a conjugate comprising flavin adenine dinucleotide and a ligand, (iii) apoglucose oxidase and (iv) a substrate, wherein the conjugate is capable of binding to the apoglucose oxidase to form a holoenzyme which, in turn, is capable of catalyzing the conversion of the substrate into a detectable product; and (2) measuring the detectable product, the improvement comprising carrying out the assay in a liquid solution at about 37° C. in the presence of at least a 250 mM final concentration of a polyhydric aldehyde or polyhydric alcohol, the polyhydric aldehyde or polyhydric alcohol being the same as or different from the substrate, whereby the formation of the holoenzyme from the apoglucose oxidase is provided.
Claim 3
Dependent Claim
10 words
The process of claim 1 wherein the ligand is theophylline.
Claim 4
Dependent Claim
27 words
The process of claim 1 wherein the polyhydric aldehyde or alcohol is a mono- or disaccharide or is a polyhydric alcohol having up to six carbon atoms.
Claim 5
Dependent Claim
15 words
The process of claim 3 wherein the polyhydric aldehyde or alcohol is glucose or sucrose.
Claim 6
Dependent Claim
26 words
The process of claim 3 wherein the polyhydric aldehyde or alcohol is a six-carbon monosaccharide or a disaccharide and the concentration thereof is about 1000 mM.
Claim 7
Dependent Claim
15 words
The process of claim 5 wherein the polyhydric aldehyde or alcohol is glucose or sucrose.
Claim 8
Dependent Claim
10 words
The process of claim 6 wherein the ligand is theophylline.
Claim 9
Dependent Claim
28 words
The process of claim 3 wherein the polyhydric aldehyde or alcohol is a six-carbon monosaccharide or a dissacharide and the concentration thereof is at least about 500 mM.
Claim 10
Dependent Claim
15 words
The process of claim 8 wherein the polyhydric aldehyde or alcohol is glucose or sucrose.
Claim 11
Dependent Claim
10 words
The process of claim 8 wherein the ligand is theophylline.
References Cited (Backward Citations)
US4366243A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-09-25 | 1988-03-26 | 1988-09-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-09-25 | 1992-03-26 | 1992-09-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-09-25 | 1996-03-26 | 1996-09-25 | $7700.00 | Window Closed / Lapsed |