Patent US1287156A successfully refreshed from Google Patents.
Patent US5064943A successfully refreshed from Google Patents.
US5064943A
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Method for solubilization and naturation of somatotropin
Assignee: Wyeth Holdings LLC
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Filing: 1988-12-16
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Granted: 1991-11-12
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Expires: 2008-12-16
Abstract
Abstract A method for solubilization and naturation of somatotropin using an aqueous alkaline solution results in lower dimer formation and eliminates denaturants and separate renaturation steps and agents.
Inventors
- 👤 Kevin M. McCoy
- 👤 Robert A. Frost
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 19:11
CPC Classifications
C; C12; C12N; C12N9/00; C07; C07K; C07K14/00; C07K14/435; C07K14/575; C07K14/61
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
109 words
A method for solubilization and naturation of recombinantly derived somatotropin which comprises: (a) dispersing somatotropin refractile bodies into water in concentration of about 5 g/l; (b) adjusting the pH to range from pH 11.5 to pH 12.5; (c) maintaining the pH range for about two minutes to twenty minutes to solubilize the refractile bodies; (d) readjusting the pH of the solution to a range from 11 to 12; and (e) maintaining the solution at the readjusted pH range for about five hours to twelve hours to effect naturation of the somatotropin, and wherein all steps of the process are carried out in the absence of chaotropic and denaturing agents.
Claim 2
Independent Claim
109 words
A method for solubilization and naturation of recombinantly derived somatotropin which comprises: (a) dispersing somatotropin refractile bodies into water in concentration of about 5 g/l; (b) adjusting the pH to range from pH 11.5 to pH 12.5; (c) maintaining the pH range for about two minutes to twenty minutes to solubilize the refractile bodies; (d) readjusting the pH of the solution to a range from 11 to 12; and (e) maintaining the solution at the readjusted pH range for about five hours to twelve hours to effect naturation of the somatotropin, and wherein all steps of the process are carried out in the absence of chaotropic and denaturing agents.
Claim 3
Dependent Claim
14 words
The method as in claim 1 wherein the somatotropin is bovine or porcine somatotropin.
Claim 4
Dependent Claim
41 words
The method as claimed in claim 1 wherein the pH in step (b) is adjusted to a range from about pH 12.0 to pH 12.2 and the pH range is readjusted in step (d) to about pH 11.3 to pH 11.7.
Claim 5
Dependent Claim
19 words
The method as in claim 2 wherein the concentration in step (a) is about 0.5 g/l to 5 g/l.
Claim 6
Dependent Claim
36 words
The method as in claim 4 wherein the concentration in step (a) is about 2.5 g/l, the pH is readjusted in step (d) to pH 11.5 and the time in step (e) is about 10 hours.
Claim 7
Independent Claim
75 words
A method for solubilization and naturation of somatotropin which comprises: (a) dispersing somatotropin refractile bodies into water in concentration of less than about 5 g/l; (b) adjusting the pH to a range from pH 11.5 to pH 12.5; and (c) maintaining the pH range for about two minutes to twenty minutes to effect naturation of the somatotropin, and wherein all steps of the process are carried out in the absence of chaotropic and denaturing agents.
Claim 8
Independent Claim
75 words
A method for solubilization and naturation of somatotropin which comprises: (a) dispersing somatotropin refractile bodies into water in concentration of less than about 5 g/l; (b) adjusting the pH to a range from pH 11.5 to pH 12.5; and (c) maintaining the pH range for about two minutes to twenty minutes to effect naturation of the somatotropin, and wherein all steps of the process are carried out in the absence of chaotropic and denaturing agents.
Claim 9
Dependent Claim
48 words
The method in claim 6 wherein the pH is about pH 12.0 to about pH 12.2, the concentration is about 0.5 g/l to about 5 g/l, and the time is about 5 hours to about 12 hours at a temperature of about 20° C. to about 30° C.
References Cited (Backward Citations)
WO1983004418A1
Cited
US4511503A
Cited
EP0122080A1
Cited
US4652630A
Cited
US4652630B1
Cited
US4677196A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-11-11 | 1995-05-13 | 1995-11-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-11-11 | 1999-05-13 | 1999-11-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-11-11 | 2003-05-13 | 2003-11-12 | $7700.00 | Window Closed / Lapsed |