US5063153A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Integral multilayer analytical element
Assignee: Fujifilm Holdings Corp
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Filing: 1987-10-09
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Granted: 1991-11-05
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Expires: 2007-10-09
Abstract
Abstract An improvement of a dry integral multilayer analytical element having functional layers which contain dehydrogenase, oxidized nicotinamide coenzyme, an electron transport compound and an electron acceptable dye-forming compound is disclosed. The improvement the analytical element resides in that an alkali agent or an alkaline buffer is contained in a layer which is different from a layer or layers containing the oxidized nicotinamide coenzyme and electron acceptable dye-forming compound.
Inventors
- 👤 Fuminori Arai
- 👤 Takeshi Igarashi
- 👤 Mitsutoshi Tanaka
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 22:25
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/32; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/525
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
135 words
A process for the preparation of a dry integral multilayer analytical element having reagent layers containing a dehydrogenase, oxidized nicotinamide coenzyme, an electron transport compound, an electron acceptable dye-forming compound, an alkali agent or an alkaline buffer selected from the group consisting of 2-amino-2-methyl-1,3-propanediol, 2-amino-2-ethyl-1,3-propanediol, diethanolamine, ethanolamine, 2-amino-2-methyl-1-propanediol, tris(hydroxymethyl)aminomethane and trimethylamine provided on a light-transmissive, water-impermeable support which comprises the steps of: forming on said support a reagent layer containing at least said oxidized nicotinamide coenzyme and electron acceptable dye-forming compound dispersed in a hydrophilic polymer; forming a spreading layer having a metering effect; coating a solution or dispersion containing at least said alkali agent or alkaline buffer in an organic solvent which causes substantially no swelling of the hydrophilic polymer in the underlying reagent layer over the spreading layer; and drying the coated layers.
Claim 2
Dependent Claim
135 words
A process for the preparation of a dry integral multilayer analytical element having reagent layers containing a dehydrogenase, oxidized nicotinamide coenzyme, an electron transport compound, an electron acceptable dye-forming compound, an alkali agent or an alkaline buffer selected from the group consisting of 2-amino-2-methyl-1,3-propanediol, 2-amino-2-ethyl-1,3-propanediol, diethanolamine, ethanolamine, 2-amino-2-methyl-1-propanediol, tris(hydroxymethyl)aminomethane and trimethylamine provided on a light-transmissive, water-impermeable support which comprises the steps of: forming on said support a reagent layer containing at least said oxidized nicotinamide coenzyme and electron acceptable dye-forming compound dispersed in a hydrophilic polymer; forming a spreading layer having a metering effect; coating a solution or dispersion containing at least said alkali agent or alkaline buffer in an organic solvent which causes substantially no swelling of the hydrophilic polymer in the underlying reagent layer over the spreading layer; and drying the coated layers.
Claim 3
Dependent Claim
128 words
A process for the preparation of a dry integral multilayer analytical element having reagent layers containing a dehydrogenase, oxidized nicotinamide coenzyme, an electron transport compound, an electron acceptable dye-forming compound and an alkali agent or alkaline buffer selected from the group consisting of 2-amino-2-methyl-1,3-propanediol, 2-amino-2-ethyl-1,3-propanediol, diethanolamine, ethanolamine, 2-amino-2-methyl-1-propanediol, tris(hydroxymethyl)aminomethane and trimethylamine provided on a light-transmissive, water-impermeable support which comprises the steps of: forming on said support a reagent layer containing at least said oxidized nicotinamide coenzyme and electron acceptable dye-forming compound dispersed in a hydrophilic polymer; coating a solution or dispersion containing at least said alkali agent or alkaline buffer in an organic solvent which causes substantially no swelling of the hydrophilic polymer in the underlying reagent layer over the underlying reagent layer; and drying the coated layers.
Claim 4
Dependent Claim
128 words
A process for the preparation of a dry integral multilayer analytical element having reagent layers containing a dehydrogenase, oxidized nicotinamide coenzyme, an electron transport compound, an electron acceptable dye-forming compound and an alkali agent or alkaline buffer selected from the group consisting of 2-amino-2-methyl-1,3-propanediol, 2-amino-2-ethyl-1,3-propanediol, diethanolamine, ethanolamine, 2-amino-2-methyl-1-propanediol, tris(hydroxymethyl)aminomethane and trimethylamine provided on a light-transmissive, water-impermeable support which comprises the steps of: forming on said support a reagent layer containing at least said oxidized nicotinamide coenzyme and electron acceptable dye-forming compound dispersed in a hydrophilic polymer; coating a solution or dispersion containing at least said alkali agent or alkaline buffer in an organic solvent which causes substantially no swelling of the hydrophilic polymer in the underlying reagent layer over the underlying reagent layer; and drying the coated layers.
Claim 5
Dependent Claim
29 words
The process as defined in claim 1 wherein said hydrophilic polymer is selected from the group consisting of gelatin, hydrophilic gelatin derivative, agarose, polyacrylamide, polyvinyl alcohol and polyvinyl pyrrolidone.
Claim 6
Dependent Claim
25 words
The process as defined in claim 1 wherein said organic solvent is selected from the group consisting of alcohols, dialkyl ketones, dialkylethers and cyclic ethers.
Claim 7
Dependent Claim
29 words
The process as defined in claim 1 wherein said organic solvent is selected from the group consisting of methanol, ethanol, propanol, butanol, isopropyl alcohol, acetone, dimethylether, tetrahydrofuran and dioxane.
Claim 8
Dependent Claim
29 words
The process as defined in claim 2 wherein said hydrophilic polymer is selected from the group consisting of gelatin, hydrophilic gelatin derivative, agarose, polyacrylamide, polyvinyl alcohol and polyvinyl pyrrolidone.
Claim 9
Dependent Claim
25 words
The process as defined in claim 2 wherein said organic solvent is selected from the group consisting of alcohols, dialkyl ketones, dialkylethers and cyclic ethers.
Claim 10
Dependent Claim
30 words
The process as defined in claim 2 wherein said organic solvent is selected from the group consisting of methanol, ethanol, propanol, butanol, isopropyl alcohol, acetone, dimethyl ether, tetrahydrofuran and dioxane.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-11-04 | 1995-05-06 | 1995-11-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-11-04 | 1999-05-06 | 1999-11-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-11-04 | 2003-05-06 | 2003-11-05 | $7700.00 | Window Closed / Lapsed |