US4273870A
Expired / Lapsed
US
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Method and composition for the determination of glycerol
Assignee: Roche Diagnostics GmbH
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Filing: 1979-07-05
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Granted: 1981-06-16
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Expires: 1999-07-05
Abstract
Abstract Method and composition for the determination of glycerol using glycerin dehydrogenase, an electron transfer agent, NAD and a tetrazolium salt and, in addition, at least one microgram of zinc, in the form of a zinc salt, per 50 U of glycerol dehydrogenase.
Inventors
- 👤 Hans Mollering
- 👤 Siegfried Looser
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 15:59
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/32
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
75 words
Method for the determination of glycerol, which method comprises contacting a sample with glycerol dehydrogenase, an electron transfer agent, NAD and a tetrazolium salt in amounts suitable for enzymatic activity and, in addition, at least one microgram of zinc, in the form of a water soluble zinc salt, per 50 units of glycerol dehydrogenase under conditions suitable for enzymatic activity; and determining the glycerol initially present in the sample by measuring the formation of dye.
Claim 2
Dependent Claim
75 words
Method for the determination of glycerol, which method comprises contacting a sample with glycerol dehydrogenase, an electron transfer agent, NAD and a tetrazolium salt in amounts suitable for enzymatic activity and, in addition, at least one microgram of zinc, in the form of a water soluble zinc salt, per 50 units of glycerol dehydrogenase under conditions suitable for enzymatic activity; and determining the glycerol initially present in the sample by measuring the formation of dye.
Claim 3
Dependent Claim
14 words
Method as claimed in claim 1 wherein said zinc salt is ZnCl 2 .
Claim 4
Dependent Claim
47 words
Reagent for the determination of glycerol comprising glycerol dehydrogenase, an electron transfer agent, NAD, tetrazolium salt, and buffer, in amounts suitable for enzymatic acitivity; and at least one microgram of zinc, in the form of a water soluble zinc salt, for every 50 units of glycerol dehydrogenase.
Claim 5
Dependent Claim
47 words
Reagent for the determination of glycerol comprising glycerol dehydrogenase, an electron transfer agent, NAD, tetrazolium salt, and buffer, in amounts suitable for enzymatic acitivity; and at least one microgram of zinc, in the form of a water soluble zinc salt, for every 50 units of glycerol dehydrogenase.
Claim 6
Dependent Claim
14 words
Reagent as claimed in claim 3 wherein said zinc salt is ZnCl 2 .
Claim 7
Dependent Claim
13 words
Reagent as claimed in claim 3 wherein said zinc salt is zinc sulfate.
Claim 8
Dependent Claim
13 words
Reagent as claimed in claim 3 wherein said zinc salt is zinc acetate.
Claim 9
Dependent Claim
30 words
Reagent as claimed in claim 3 wherein said zinc salt is contained in an amount of from 0.01 to 1.0 millimoles of zinc salt per liter of reagent test mixture.
Claim 10
Dependent Claim
53 words
Reagent as claimed in claim 3 containing 5 to 20 units/ml of glycerol dehydrogenase, 0.2 to 1.5 units/ml of diaphorase, 0.7+5 millimoles/l of NAD 0.1 to 0.5 millimoles/l of tetrazolium salt, 0.05 to 0.2 moles/l of buffer, pH 7.5 to 9.5, 0 to 0.5% detergent, and 0.01 to 1.0 millimoles/l of zinc salt.
Claim 11
Dependent Claim
13 words
Reagent as claimed in claim 8 wherein said zinc salt is zinc chloride.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-06-15 | 1984-12-15 | 1985-06-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-06-15 | 1988-12-15 | 1989-06-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-06-15 | 1992-12-15 | 1993-06-16 | $7700.00 | Window Closed / Lapsed |