US5059522A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Intrinsic enzyme dot blot immunoassay or indentification of microorganisms
Assignee: Individual
•
Filing: 1988-08-18
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Granted: 1991-10-22
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Expires: 2008-08-18
Abstract
Abstract A method for performing an assay to differentiate between related taxa of micoorganisms, comprising the steps of providing a support having a plurality of zones, each zone comprising immobilized antibody to an enzyme found in a particular microorganism, wherein the antibody in the zone is specific to the enzyme, but not to corresponding enzyme of other taxa to be determined in the assay; contacting the zones with a sample suspected of containing one of the particular microorganisms; permitting enzyme in the sample to become bound by antibody in one or more of the zones; and determining which of the taxa were present in the sample by determining to which of the zones the enzyme has become bound. The determining step may comprise adding a substrate for the enzyme to the support after the enzyme has become bound thereto, wherein the substrate, after being acted on by the enzyme, creates a detectable signal. Also disclosed are an assay kit, an immunoreactive support, and a method for preparing the support, all for use in the method of the present invention.
Inventors
- 👤 Lawrence G. Wayne
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 18:19
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/569; G01N33/56911; Y; Y10; Y10S; Y10S435/00; Y10S435/967; Y10S435/97; Y10S435/973; Y10S435/975; Y10S436/00; Y10S436/807; Y10S436/809
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
156 words
A method for performing an assay to determine the presence of one or more related taxa of microorganisms, comprising the steps of: providing a support having a plurality of first zones, each said first zone comprising immobilized antibody to an enzyme found in a different taxon of microorganism, wherein each antibody in each of said first zones is specific to said enzyme of one taxon, but not to a corresponding enzyme of other related taxa to be determined in said assay, and a control zone having antibody immobilized thereto which binds said corresponding enzyme of all said related taxa; contacting said zones with a sample suspected of containing one of said microorganisms or an extract thereof; permitting enzyme in said sample to become bound by antibody in one or more of said zones; and determining which of said taxa were present in said sample by determining to which of said zones said enzyme has become bound.
Claim 2
Independent Claim
156 words
A method for performing an assay to determine the presence of one or more related taxa of microorganisms, comprising the steps of: providing a support having a plurality of first zones, each said first zone comprising immobilized antibody to an enzyme found in a different taxon of microorganism, wherein each antibody in each of said first zones is specific to said enzyme of one taxon, but not to a corresponding enzyme of other related taxa to be determined in said assay, and a control zone having antibody immobilized thereto which binds said corresponding enzyme of all said related taxa; contacting said zones with a sample suspected of containing one of said microorganisms or an extract thereof; permitting enzyme in said sample to become bound by antibody in one or more of said zones; and determining which of said taxa were present in said sample by determining to which of said zones said enzyme has become bound.
Claim 3
Dependent Claim
44 words
The method of claim 1, wherein said determining step comprises: adding a substrate for said enzyme to said support after said enzyme has become bound thereto, wherein said substrate, after being acted on by said enzyme, creates a detectable signal; and detecting said signal.
Claim 4
Dependent Claim
129 words
An assay kit for differentiating between related taxa of microorganisms, comprising: a solid support; a plurality of first zones on said support, each said first zone comprising immobilized antibody to an enzyme found in a different taxon of microorganism, wherein each antibody in each of said first zones is specific to said enzyme of one taxon, but not to a corresponding enzyme of other related taxa to be determined in said assay, and a control zone to which control antibody is immobilized, wherein said control antibody is specific to said corresponding enzyme of all said taxa to be differentiated; and substrate for said enzyme to which said antibodies in said zones are specific, wherein said substrate, when acted on by enzyme bound by said antibodies, creates a detectable signal.
Claim 5
Dependent Claim
129 words
An assay kit for differentiating between related taxa of microorganisms, comprising: a solid support; a plurality of first zones on said support, each said first zone comprising immobilized antibody to an enzyme found in a different taxon of microorganism, wherein each antibody in each of said first zones is specific to said enzyme of one taxon, but not to a corresponding enzyme of other related taxa to be determined in said assay, and a control zone to which control antibody is immobilized, wherein said control antibody is specific to said corresponding enzyme of all said taxa to be differentiated; and substrate for said enzyme to which said antibodies in said zones are specific, wherein said substrate, when acted on by enzyme bound by said antibodies, creates a detectable signal.
Claim 6
Dependent Claim
14 words
The kit of claim 3, wherein said support has at least three first zones.
Claim 7
Dependent Claim
96 words
An immunoreactive support for use in differentiating between related taxa of microorganisms, comprising: a solid support; a plurality of first zones of immobilized antibody on said support, each of which is specific to an enzyme of one of said taxa, but not to a corresponding enzyme of other of said related taxa, which enzyme is capable of acting on a substrate to produce a detectable signal; and a control zone having antibody immobilized thereto which binds said corresponding enzyme of all of said taxa to which antibody in said first zones on said support is specific.
Claim 8
Dependent Claim
96 words
An immunoreactive support for use in differentiating between related taxa of microorganisms, comprising: a solid support; a plurality of first zones of immobilized antibody on said support, each of which is specific to an enzyme of one of said taxa, but not to a corresponding enzyme of other of said related taxa, which enzyme is capable of acting on a substrate to produce a detectable signal; and a control zone having antibody immobilized thereto which binds said corresponding enzyme of all of said taxa to which antibody in said first zones on said support is specific.
Claim 9
Dependent Claim
14 words
The support of claim 5, wherein said support has at least three first zones.
Claim 10
Independent Claim
99 words
A method for preparing a support for use in differentiating microorganisms, comprising the steps of: providing a solid support; binding a plurality of different first antibody spots thereto, each said first spot being specific to the same enzyme of a different taxon of microorganism, but not to a corresponding enzyme of other related taxa, said enzyme being capable of acting on a substrate to produce a detectable signal; and binding a control antibody spot thereto, which control spot binds said corresponding enzyme of all of said taxa to which antibody in said first spots on said support is specific.
Claim 11
Independent Claim
99 words
A method for preparing a support for use in differentiating microorganisms, comprising the steps of: providing a solid support; binding a plurality of different first antibody spots thereto, each said first spot being specific to the same enzyme of a different taxon of microorganism, but not to a corresponding enzyme of other related taxa, said enzyme being capable of acting on a substrate to produce a detectable signal; and binding a control antibody spot thereto, which control spot binds said corresponding enzyme of all of said taxa to which antibody in said first spots on said support is specific.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-10-21 | 1995-04-22 | 1995-10-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-10-21 | 1999-04-22 | 1999-10-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-10-21 | 2003-04-22 | 2003-10-22 | $7700.00 | Window Closed / Lapsed |