Method for diagnostically imaging lesions in the brain inside a blood-brain barrier

Claim 1

A method for diagnostically imaging a naturally occurring brain lesion in a warm-blooded animal having a brain situated inside a blood-brain barrier having an ambient permeability level, the method comprising the steps: administering a first chemical agent to said animal at a location outside the blood-brain barrier, where the first chemical agent is capable of increasing the permeability of the blood-brain barrier from the ambient level to an elevated permeability level; administering a second chemical agent to said animal at a location outside the blood-brain barrier following the administration of said first chemical agent, said second chemical agent being capable of binding preferentially to a naturally occurring brain lesion and of passing through the animal's blood-brain barrier to the brain lesion when the blood-brain barrier is at the elevated permeability level but not at the ambient permeability level, said second chemical agent comprised of molecules having at least one labeling agent attached to each said molecule; allowing the animal to eliminate any amount of the second chemical agent with attached labeling agent not bound to said brain lesion; and analyzing said brain of said animal for the presence of said labeling agent bound to the brain lesion in order to determine the size, type, and character of said brain lesion.

Claim 2

A method for diagnostically imaging a naturally occurring brain lesion in a warm-blooded animal having a brain situated inside a blood-brain barrier having an ambient permeability level, the method comprising the steps: administering a first chemical agent to said animal at a location outside the blood-brain barrier, where the first chemical agent is capable of increasing the permeability of the blood-brain barrier from the ambient level to an elevated permeability level; administering a second chemical agent to said animal at a location outside the blood-brain barrier following the administration of said first chemical agent, said second chemical agent being capable of binding preferentially to a naturally occurring brain lesion and of passing through the animal's blood-brain barrier to the brain lesion when the blood-brain barrier is at the elevated permeability level but not at the ambient permeability level, said second chemical agent comprised of molecules having at least one labeling agent attached to each said molecule; allowing the animal to eliminate any amount of the second chemical agent with attached labeling agent not bound to said brain lesion; and analyzing said brain of said animal for the presence of said labeling agent bound to the brain lesion in order to determine the size, type, and character of said brain lesion.

Claim 7

A method for diagnostically imaging a naturally occurring brain lesion in a warm-blooded animal having a blood-brain barrier having an ambient permeability level, the method comprising the steps of: administering a first chemical agent to said animal at a location outside the blood-brain barrier, where the first chemical agent is capable of increasing the permeability of the blood-brain barrier from the ambient level to an elevated permeability level and comprises a hypertonic solution of a material selected from the group consisting of mannitol, arabinose, and glycerol; administering a second chemical agent to said animal at a location outside the blood-brain barrier following administration of said first chemical agent, said second chemical agent being capable of binding preferentially to a naturally occurring brain lesion tissues and of passing through the animal's blood-brain barrier to the brain lesion when the blood-brain barrier is at the elevated permeability level but not at the ambient permeability level, where said second chemical agent comprises monoclonal antibody molecules each having at least one labeling agent comprising gadolinium-DTPA attached to each said monoclonal antibody molecule; allowing the animal to eliminate any amount of the second chemical agent with attached labeling agent not bound to said brain lesion; and analyzing said brain of said animal for the presence of said labeling agent bound to the brain lesion in order to determine the size, type, and character of said brain lesion, said analyzing comprising the step of conducting a magnetic resonance imaging scan of said brain.

Claim 8

A method for diagnostically imaging a naturally occurring brain lesion in a warm-blooded animal having a blood-brain barrier having an ambient permeability level, the method comprising the steps of: administering a first chemical agent to said animal at a location outside the blood-brain barrier, where the first chemical agent is capable of increasing the permeability of the blood-brain barrier from the ambient level to an elevated permeability level and comprises a hypertonic solution of a material selected from the group consisting of mannitol, arabinose, and glycerol; administering a second chemical agent to said animal at a location outside the blood-brain barrier following administration of said first chemical agent, said second chemical agent being capable of binding preferentially to a naturally occurring brain lesion tissues and of passing through the animal's blood-brain barrier to the brain lesion when the blood-brain barrier is at the elevated permeability level but not at the ambient permeability level, where said second chemical agent comprises monoclonal antibody molecules each having at least one labeling agent comprising gadolinium-DTPA attached to each said monoclonal antibody molecule; allowing the animal to eliminate any amount of the second chemical agent with attached labeling agent not bound to said brain lesion; and analyzing said brain of said animal for the presence of said labeling agent bound to the brain lesion in order to determine the size, type, and character of said brain lesion, said analyzing comprising the step of conducting a magnetic resonance imaging scan of said brain.