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Ultrapurification of factor IX and other vitamin K-dependent proteins
Assignee: Scripps Research Institute
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Filing: 1988-11-23
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Granted: 1991-10-08
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Expires: 2008-11-23
Abstract
Abstract A method of recovering active, highly purified and concentrated vitamin K-dependent proteins from plasma, concentrate or mixtures of proteins produced by recombinant DNA technology using an immuno adsorbent comprising a monoclonal antibody.
Inventors
- 👤 Theodore S. Zimmerman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 07:13
CPC Classifications
B; B01; B01J; B01J20/00; B01J20/30; B01J20/32; B01J20/3231; B01J20/3242; B01J20/3268; B01J20/3272; B01J20/3274; B01D; B01D15/00; B01D15/08; B01D15/26; B01D15/38; B01D15/3804; B01D15/3809; B01J20/281; B01J20/286; B01J20/3202; B01J20/3204; B01J20/3206; B01J20/3208; B01J20/3212; C; C07; C07K; C07K14/00; C07K14/435; C07K14/745; C07K16/00; C07K16/18; C07K16/36; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6424; C12N9/644; C12Y; C12Y304/00; C12Y304/21; C12Y304/21022; A; A61; A61K; A61K39/00; B01D15/42; B01D15/424; B01D15/426; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/829; Y10S530/83
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
89 words
A method of recovering an active, highly purified and concentrated vitamin K-dependent protein, from a source comprising a mixture of said vitamin K-dependent protein with one or more other proteins, comprising: (a) adsorbing said vitamin K-dependent protein from said source onto particles bound to a monoclonal antibody specific to said vitamin K-dependent protein, (b) removing the source depleted in said vitamin K-dependent protein from the particles with an aqueous buffered solution containing lithium chloride, and then (c) eluting the adsorbed vitamin K-dependent protein from particles with a buffered eluant.
Claim 2
Independent Claim
89 words
A method of recovering an active, highly purified and concentrated vitamin K-dependent protein, from a source comprising a mixture of said vitamin K-dependent protein with one or more other proteins, comprising: (a) adsorbing said vitamin K-dependent protein from said source onto particles bound to a monoclonal antibody specific to said vitamin K-dependent protein, (b) removing the source depleted in said vitamin K-dependent protein from the particles with an aqueous buffered solution containing lithium chloride, and then (c) eluting the adsorbed vitamin K-dependent protein from particles with a buffered eluant.
Claim 3
Dependent Claim
17 words
The method of claim 1 wherein said source is plasma, a plasma fraction, or a plasma concentrate.
Claim 4
Dependent Claim
22 words
The method of claim 1 wherein said source has been expressed by a cell containing a gene for producing said selected protein.
Claim 5
Dependent Claim
12 words
The method of claim 1 wherein said selected protein is Factor IX.
Claim 6
Dependent Claim
12 words
The method of claim 2 wherein said selected protein is Factor IX.
Claim 7
Dependent Claim
12 words
The method of claim 3 wherein said selected protein is Factor IX.
Claim 8
Dependent Claim
20 words
The method according to claim 1, wherein the buffered eluant used in step (c) is sodium thiocyanate in aqueous solution.
Claim 9
Dependent Claim
20 words
The method according to claim 1, wherein the buffered eluant used in step (c) is potassium bromide in aqueous solution.
Claim 10
Dependent Claim
16 words
The method according to claim 1, wherein the buffered eluant used in step (c) is diethylamine.
Claim 11
Dependent Claim
20 words
The method according to claim 1, wherein the buffered eluant used in step (c) is calcium chloride in aqueous solution.
Claim 12
Dependent Claim
15 words
The method according to claim 1, wherein said adsorbent particles in step (a) are agarose.
Claim 13
Dependent Claim
39 words
The method of claim 1 wherein the buffered eluant used in step (c) is diethylamine or an aqueous solution of a solute selected from the group consisting of calcium chloride, sodium thiocyanate, potassium bromide, acetic acid, and glycine-hydrochloric acid.
Claim 14
Independent Claim
94 words
A method of recovering active, highly purified and concentrated Factor IX, from a source comprising a mixture of Factor IX with one or more other proteins, comprising: (a) contacting said source for a period of between 1 to 6 hours with a monoclonal antibody specific to Factor IX which has been coupled to a solid substrate, (b) eluting the source depleted in Factor IX from said substrate with an aqueous buffered solution containing lithium chloride, and then (c) eluting the adsorbed Factor IX from said substrate with a buffered eluant to recover Factor IX.
Claim 15
Independent Claim
94 words
A method of recovering active, highly purified and concentrated Factor IX, from a source comprising a mixture of Factor IX with one or more other proteins, comprising: (a) contacting said source for a period of between 1 to 6 hours with a monoclonal antibody specific to Factor IX which has been coupled to a solid substrate, (b) eluting the source depleted in Factor IX from said substrate with an aqueous buffered solution containing lithium chloride, and then (c) eluting the adsorbed Factor IX from said substrate with a buffered eluant to recover Factor IX.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-10-07 | 1995-04-08 | 1995-10-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-10-07 | 1999-04-08 | 1999-10-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-10-07 | 2003-04-08 | 2003-10-08 | $7700.00 | Window Closed / Lapsed |