US4361509A
Expired / Lapsed
US
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Ultrapurification of factor VIII using monoclonal antibodies
Assignee: Scripps Research Institute
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Filing: 1981-12-14
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Granted: 1982-11-30
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Expires: 2001-12-14
Abstract
Abstract A method of preparing high purity procoagulant protein comprising the steps of (a) adsorbing a VIII:C/VIII:RP complex from a plasma or commercial concentrate source of factor VIII onto agarose beads bound to a monoclonal antibody specific to VIII:RP, (b) eluting VIII:C with a salt solution, (c) adsorbing the eluted VIII:C on an animohexyl agarose column and eluting the VIII:C with a salt solution.
Inventors
- 👤 Theodore S. Zimmerman
- 👤 Carol A. Fulcher
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 22:42
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/745; C07K14/755; A; A61; A61P; A61P7/00; A61P7/04; A61K; A61K38/00; Y; Y10; Y10S; Y10S530/00; Y10S530/808; Y10S530/81; Y10S530/812; Y10S530/813; Y10S530/814; Y10S530/827; Y10S530/829; Y10S530/83
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
70 words
An improved method of preparing Factor VIII procoagulant activity protein comprising the steps of (a) adsorbing a VIII:C/VIII:RP complex from a plasma or commercial concentrate source onto particles bound to a monoclonal antibody specific to VIII:RP, (b) eluting the VIII:C, (c) adsorbing the VIII:C obtained in step (b) in another adsorption to concentrate and further purify same, (d) eluting the adsorbed VIII:C, and (e) recovering highly purified and concentrated VIII:C.
Claim 2
Dependent Claim
70 words
An improved method of preparing Factor VIII procoagulant activity protein comprising the steps of (a) adsorbing a VIII:C/VIII:RP complex from a plasma or commercial concentrate source onto particles bound to a monoclonal antibody specific to VIII:RP, (b) eluting the VIII:C, (c) adsorbing the VIII:C obtained in step (b) in another adsorption to concentrate and further purify same, (d) eluting the adsorbed VIII:C, and (e) recovering highly purified and concentrated VIII:C.
Claim 3
Dependent Claim
21 words
A method according to claim 1, wherein the elutant used in each of steps (b) and (d) is a saline solution.
Claim 4
Dependent Claim
13 words
The method according to claim 2, wherein the saline solution is calcium chloride.
Claim 5
Dependent Claim
29 words
The method according to claim 3, wherein the concentration of said calcium chloride solution used in steps (b) and (d) ranges from about 0.25 M to about 0.5 M.
Claim 6
Dependent Claim
15 words
The method according to claim 1, wherein said adsorbent particles in step (a) are agarose.
Claim 7
Dependent Claim
17 words
The method according to claim 1, wherein aminohexyl agarose is employed in step (c) as the adsorbent.
Claim 8
Dependent Claim
50 words
The method according to claim 6, wherein calcium chloride solution is employed as the elutant in steps (b) and (d), concentration of said calcium chloride solution ranging from about 0.25 M to about 0.5 M in step (b) and from about 0.25 M to about 0.5 M in step (d).
Claim 9
Dependent Claim
22 words
An improved immunoadsorbent for isolation and purification of VIII:C from VIII:C/VIII:RP comprising a monoclonal antibody specific to VIII:RP bound to solid particles.
Claim 10
Dependent Claim
22 words
An improved immunoadsorbent for isolation and purification of VIII:C from VIII:C/VIII:RP comprising a monoclonal antibody specific to VIII:RP bound to solid particles.
Claim 11
Dependent Claim
13 words
The improved immunoadsorbent of claim 8, wherein said solid particles comprise a resin.
Claim 12
Dependent Claim
11 words
The improved immunoadsorbent of claim 9, wherein said resin comprises agarose.
Claim 13
Dependent Claim
12 words
The improved immunoadsorbent of claim 10, wherein said agarose is cross-linked agarose.
Claim 14
Dependent Claim
25 words
The improved immunoadsorbent of claim 11, wherein said immunoadsorbent has a coupling density of 3 to 4 g of monoclonal antibody per liter of agarose.
Claim 15
Dependent Claim
14 words
Highly purified and concentrated VIII:C prepared in accordance with the method of claim 1.
Claim 16
Dependent Claim
14 words
Highly purified and concentrated VIII:C prepared in accordance with the method of claim 6.
Claim 17
Dependent Claim
48 words
In a method for purifying Factor VIII procoagulant activity protein from plasma or concentrate, the improvement comprising the step of passing said plasma or concentrate through a chromatographic type column having adsorbent to which is bound monoclonal antibodies which is specific to VIII:RP and eluting the VIII-C therefrom.
Claim 18
Dependent Claim
48 words
In a method for purifying Factor VIII procoagulant activity protein from plasma or concentrate, the improvement comprising the step of passing said plasma or concentrate through a chromatographic type column having adsorbent to which is bound monoclonal antibodies which is specific to VIII:RP and eluting the VIII-C therefrom.
Claim 19
Dependent Claim
18 words
The method according to claim 15, wherein said adsorbent is agarose and said elutant is a saline solution.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-11-29 | 1986-05-31 | 1986-11-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-11-29 | 1990-05-31 | 1990-11-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-11-29 | 1994-05-31 | 1994-11-30 | $7700.00 | Window Closed / Lapsed |