US5039488A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Devices for amino acid sequence determination
Assignee: Genentech Inc
•
Filing: 1990-09-04
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Granted: 1991-08-13
•
Expires: 2010-09-04
Abstract
Abstract A device for the determination of amino acid sequence of a polypeptide comprises two new features offering great advantages in the cost and efficiency of operation of amino acid sequencers. The sequencer is provided with the capability for the bidirectional flow of sequencing reagents and contains a sample chamber having a bicompositional adsorbent for the polypeptide.
Inventors
- 👤 William J. Kohr
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 04:11
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/68; G01N33/6803; G01N33/6818; G01N33/6824; Y; Y10; Y10S; Y10S530/00; Y10S530/81; Y10S530/812; Y10S530/815; Y10S530/817
Claims Analysis
Total Claims: 23
Claim 1
Independent Claim
108 words
An amino acid sequencer comprising: (a) a sample chamber defining a fluid flow path and having first and second ports along said flow path; (b) a plurality of discontinuous, tandemly arrayed first and second chromatography resins for adsorption of polypeptide, said first chromatography resin including a hydrophilic resin and said second chromatography resin including a hydrophobic resin, said first and second resins being in contact with one another without substantial intermixing and disposed in said chamber in fluid communication with one another along said flow path; and (c) positive pressure means for the sequential introduction of fluid reagents into said chamber alternately through said first and second ports.
Claim 2
Dependent Claim
108 words
An amino acid sequencer comprising: (a) a sample chamber defining a fluid flow path and having first and second ports along said flow path; (b) a plurality of discontinuous, tandemly arrayed first and second chromatography resins for adsorption of polypeptide, said first chromatography resin including a hydrophilic resin and said second chromatography resin including a hydrophobic resin, said first and second resins being in contact with one another without substantial intermixing and disposed in said chamber in fluid communication with one another along said flow path; and (c) positive pressure means for the sequential introduction of fluid reagents into said chamber alternately through said first and second ports.
Claim 3
Dependent Claim
18 words
The sequencer of claim 1 wherein said first and second chromatography resins are high pressure liquid chromatography resins.
Claim 4
Dependent Claim
31 words
The sequencer of claim 1 wherein said hydrophobic resin is C-4 to C-18 alkyl substituted silica or a polymeric hydrophobic resin and said hydrophilic resin is controlled-pore glass or unsubstituted silica.
Claim 5
Dependent Claim
40 words
The sequencer of claim 1 wherein said first resin is a first substantially homogeneous mixture and said second resin is a second substantially homogeneous mixture, said first mixture and said second mixture being characterized by respective and opposing electrostatic charges.
Claim 6
Dependent Claim
23 words
The sequencer of claim 4 wherein said second mixture includes an electronegatively charged resin and said first mixture includes an electropositively charged resin.
Claim 7
Dependent Claim
25 words
The sequencer of claim 5 wherein said electropositively charged resin is a quaternary or tertiary amino substituted macroporous polyolefin or a sulfonate substituted macroporous polyolefin.
Claim 8
Dependent Claim
19 words
The sequencer of claim 1 wherein said first and second ports are at substantially opposite locations in said chamber.
Claim 9
Dependent Claim
25 words
The sequencer of claim 7 wherein said first resin is adjacent to said first port and said second resin is adjacent to said second port.
Claim 10
Dependent Claim
23 words
The sequencer of claim 8 further comprising liquid introduction means for introducing first and second liquids into said first and second ports respectively.
Claim 11
Dependent Claim
19 words
The sequencer of claim 9 wherein said chamber has means for reversibly and sealably engaging said liquid introduction means.
Claim 12
Dependent Claim
37 words
The sequencer of claim 9 further comprising first and second peptide sequencing reagents and first and second reservoirs containing said first and second peptide sequencing reagents, respectively, said liquid introduction means being in communication with said reservoirs.
Claim 13
Dependent Claim
40 words
The sequencer of claim 11 wherein said liquid introduction means comprises first introduction means for introducing said first peptide sequencing reagent into said first port and second introduction means for introducing said second peptide sequencing reagent into said second port.
Claim 14
Dependent Claim
30 words
The sequencer of claim 12 wherein said positive pressure means includes pressurizing means for conveying by pressurized gas said first and second reagents to said first and second ports, respectively.
Claim 15
Dependent Claim
28 words
The sequencer of claim 12 wherein said first peptide sequencing reagent includes a solution of phenylisothiocyanate or a derivative thereof for coupling with a peptide to be sequenced.
Claim 16
Dependent Claim
13 words
The sequencer of claim 14 wherein said solution further includes a polar solvent.
Claim 17
Dependent Claim
14 words
The sequencer of claim 15 wherein said solvent is an alkaline buffered aqueous solution.
Claim 18
Dependent Claim
17 words
The sequencer of claim 12 wherein said second peptide sequencing reagent includes a substantially nonpolar organic solvent.
Claim 19
Dependent Claim
38 words
The sequencer of claim 8 wherein said chamber is an elongated cylinder having first and second ends, said first port having an opening through said first end and said second port having an opening through said second end.
Claim 20
Dependent Claim
19 words
The sequencer of claim 1 wherein said first and second chromatography resins are substantially continuous mixtures of chromatography resins.
Claim 21
Dependent Claim
23 words
The sequencer of claim 1 further comprising thermal control means for adjusting the temperature of said chamber in coordination with reactions occurring therein.
Claim 22
Dependent Claim
46 words
The sequencer of claim 20 wherein said thermal control means is such that it can maintain the temperature of said chamber at about 55° C. for coupling of phenylisothiocyanate to a polypeptide to be sequenced and at about 0° C. to 50° C. for acid extraction.
Claim 23
Dependent Claim
29 words
The sequencer of claim 1 further comprising detection means for detecting derivatized amino acid, said detection means being in fluid communication with one of said first and second ports.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-08-12 | 1995-02-11 | 1995-08-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-08-12 | 1999-02-11 | 1999-08-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-08-12 | 2003-02-11 | 2003-08-13 | $7700.00 | Window Closed / Lapsed |