US3926800A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Temperature stabilized water jacket chromatographic column
Assignee: Beckman Coulter Inc
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Filing: 1974-01-25
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Granted: 1975-12-16
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Expires: 1994-01-25
Abstract
Abstract Thermal stability of a water jacketed chromatographic column is increased by providing an annular space of non-circulating water immediately surrounding the chromatographic column. This is accomplished by surrounding the column with a concentric tube, the internal diameter of which exceeds the external diameter of the column. A seal is provided between tube and column at the bottom. This arrangement, installed within a conventional water jacket, provides an annular space of non-circulating water. It effectively increases the thermal capacity of the chromatographic column which serves to damp out short cyclic fluctuations in temperature while permitting sufficient heat transfer between the column and the circulating water to effect closely controlled temperature under long-term load changes.
Inventors
- 👤 Donald E Stephens
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 11:28
CPC Classifications
G; G01; G01N; G01N30/00; G01N30/02; G01N30/26; G01N30/28; G01N30/30
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
158 words
IN AN ANALYSIS SYSTEM HAING AN ANALYTICAL CHROMATOGRAPHY COLUMN, THE IMPROVEMENT WHICH COMPRISES, IN CONJUNCTION WITH MEANS FOR AVOIDING FLUCTATIONS OF COLUMN TEMPERATURE WHICH HAVE LONG TIME CYCLES: SPACED TUBES WHICH INCLUDE AN INNER TUBE SURROUNDING THE COLUMN BUT SPACED THEREFROM WITH A RELATIVELY HIGH THERMAL CAPACITY MEDIUM BETWEEN THE COLUMN AND THE INNER TUBE, A WATER JACKET CONSTITUTING THE MEANS FOR AVOIDING TEMPERATURE FLUCTUATIONS WHICH HAVE LONG TIME CYCLES, AND LOCATED AROUND THE COLUMN ENCOMPASSED NY THE TUBE, WHEREBY WATER FROM THE WATER JACKET IS MAINTAINED BETWEEN THE COLUMN AND THE TUBES TO FORM THE RELATIVE HIGH THERMAL CAPACITY MEDIUM, AND MEANS FOR LIMITING FLOW OF WATER BETWEEN THE COLUMN AND THE INNER TUBE TO RETAIN A RELATIVELY HIGH THERMAL CAPACITY MEDIUM BETWEEN THE COLUMN AND THE INNER TUBE TO SUPPLEMENT THE THERMAL CAPACITY OF THE COLUMN AND MINIMIZE TEMPERATUR-INDUCED VARIATIONS IN VOLUME OF THE COLUMN, AND THUS MINIMIZING TEMPERATURE-INDUCED VARIATION IN RATE OF FLOW THE COLUMN.
Claim 2
Dependent Claim
158 words
IN AN ANALYSIS SYSTEM HAING AN ANALYTICAL CHROMATOGRAPHY COLUMN, THE IMPROVEMENT WHICH COMPRISES, IN CONJUNCTION WITH MEANS FOR AVOIDING FLUCTATIONS OF COLUMN TEMPERATURE WHICH HAVE LONG TIME CYCLES: SPACED TUBES WHICH INCLUDE AN INNER TUBE SURROUNDING THE COLUMN BUT SPACED THEREFROM WITH A RELATIVELY HIGH THERMAL CAPACITY MEDIUM BETWEEN THE COLUMN AND THE INNER TUBE, A WATER JACKET CONSTITUTING THE MEANS FOR AVOIDING TEMPERATURE FLUCTUATIONS WHICH HAVE LONG TIME CYCLES, AND LOCATED AROUND THE COLUMN ENCOMPASSED NY THE TUBE, WHEREBY WATER FROM THE WATER JACKET IS MAINTAINED BETWEEN THE COLUMN AND THE TUBES TO FORM THE RELATIVE HIGH THERMAL CAPACITY MEDIUM, AND MEANS FOR LIMITING FLOW OF WATER BETWEEN THE COLUMN AND THE INNER TUBE TO RETAIN A RELATIVELY HIGH THERMAL CAPACITY MEDIUM BETWEEN THE COLUMN AND THE INNER TUBE TO SUPPLEMENT THE THERMAL CAPACITY OF THE COLUMN AND MINIMIZE TEMPERATUR-INDUCED VARIATIONS IN VOLUME OF THE COLUMN, AND THUS MINIMIZING TEMPERATURE-INDUCED VARIATION IN RATE OF FLOW THE COLUMN.
Claim 3
Dependent Claim
46 words
The improvement defined in claim 1 wherein the tube means comprises an intermediate tube surrounding the inner tube with means for substantially preventing flow of water between the column and the inner tube and means for retarding flow of water between the inner and intermediate tubes.
Claim 4
Dependent Claim
116 words
In an analysis system having an analytical chromatography column through which sample flows and a water jacket surrounding this column in which thermostatically temperature-controlled liquid circulates, the improvement comprising: tube means surrounding the column and spaced therefrom; and flow restrictor means between the column and the tube means for limiting flow of liquid within the jacket immediately adjacent the column thereby providing a stagnant body of liquid adjacent the column for supplementing the thermal capacity of the column and thereby minimizing variations in column temperature of short term compared to temperature variations of liquid flowing directly through said water jacket and thus minimizing temperature-induced variations in volumn of the column and in rate of flow therefrom.
Claim 5
Dependent Claim
116 words
In an analysis system having an analytical chromatography column through which sample flows and a water jacket surrounding this column in which thermostatically temperature-controlled liquid circulates, the improvement comprising: tube means surrounding the column and spaced therefrom; and flow restrictor means between the column and the tube means for limiting flow of liquid within the jacket immediately adjacent the column thereby providing a stagnant body of liquid adjacent the column for supplementing the thermal capacity of the column and thereby minimizing variations in column temperature of short term compared to temperature variations of liquid flowing directly through said water jacket and thus minimizing temperature-induced variations in volumn of the column and in rate of flow therefrom.
Claim 6
Dependent Claim
38 words
The improvement defined in claim 3 wherein a seal is provided between the column and the tube means at one end of the tube means and the flow restrictor is at the other end of the tube means.
Claim 7
Dependent Claim
13 words
The improvement defined in claim 3 wherein the tube is composed of glass.
Claim 8
Dependent Claim
146 words
In a chromatograph system having a vertical chromatograph column, a thermostatically controlled water jacket around the column, the water jacket containing a circulating liquid of substantially constant temperature capable of substantially avoiding temperature fluctuations having a long time cycle, the improvement comprising: a tube also within said water jacket and surrounding the column but spaced therefrom, said tube being open at the upper end, a seal at the lower end of said tube between said column and said tube whereby water circulating through said water jacket is maintained between said column and said tube to provide a body of substantially stationary liquid around said column which maintains substantial uniformity of temperature of the column and minimizes temperature induced variations in the column volume of the order of 1-minute cycles or less, whereby the flow rate of solutions eluting from said coluMn are thereby maintained substantially constant.
Claim 9
Dependent Claim
146 words
In a chromatograph system having a vertical chromatograph column, a thermostatically controlled water jacket around the column, the water jacket containing a circulating liquid of substantially constant temperature capable of substantially avoiding temperature fluctuations having a long time cycle, the improvement comprising: a tube also within said water jacket and surrounding the column but spaced therefrom, said tube being open at the upper end, a seal at the lower end of said tube between said column and said tube whereby water circulating through said water jacket is maintained between said column and said tube to provide a body of substantially stationary liquid around said column which maintains substantial uniformity of temperature of the column and minimizes temperature induced variations in the column volume of the order of 1-minute cycles or less, whereby the flow rate of solutions eluting from said coluMn are thereby maintained substantially constant.
Claim 10
Dependent Claim
12 words
The improvement defined in claim 6 wherein the seal constitutes an O-ring.
Claim 11
Dependent Claim
127 words
In a column type analytical system involving the flow of samples through a column and delivering effluent at a fixed rate of flow from the column, the method of minimizing short term temperature change induced fluctuations in rate of flow from the column which comprises separating the space surrounding the column to form inner and outer spaces with circulating liquid flowing in the outer space and liquid flow restricted in the inner space to cause retention of a stagnant body of liquid in the inner space around the column for damping out short-term fluctuations in temperature of the column while circulating liquid of controlled temperature in outer space surrounding the column and the stagnant body of liquid to avoid long term fluctuations in temperature of the column.
Claim 12
Dependent Claim
127 words
In a column type analytical system involving the flow of samples through a column and delivering effluent at a fixed rate of flow from the column, the method of minimizing short term temperature change induced fluctuations in rate of flow from the column which comprises separating the space surrounding the column to form inner and outer spaces with circulating liquid flowing in the outer space and liquid flow restricted in the inner space to cause retention of a stagnant body of liquid in the inner space around the column for damping out short-term fluctuations in temperature of the column while circulating liquid of controlled temperature in outer space surrounding the column and the stagnant body of liquid to avoid long term fluctuations in temperature of the column.
References Cited (Backward Citations)
US3490202A
Cited
US3683589A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-12-15 | 1979-06-16 | 1979-12-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-12-15 | 1983-06-16 | 1983-12-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-12-15 | 1987-06-16 | 1987-12-16 | $7700.00 | Window Closed / Lapsed |