Abstract Biologically pure culture of Aureobasidium sp. SN-124A strain (FERM BP-1429) and artificial mutants thereof according to the present invention show the properties of forming and accumulating erythritol in a culture solution, when aerobically cultured on a liquid culture medium containing an assimilable carbon source and an assimilable nitrogen source, and are useful for the preparation of erythritol by fermentation of sugars. A method for preparing erythritol by fermentation of sugars according to the present invention comprises inoculating microorganism selected from the group consisting of Aureobasidium sp. SN-124A strain (FERM BP-1429) and mutants thereof on a liquid culture medium of pH 4 to 9 containing an assimilable carbon source and an assimilable nitrogen source, and aerobically culturing them at a temperature of 30° to 38° C. to form and accumulate erythritol in said culture medium for collection.
A biologically pure culture of Aureobasidium sp. SN-124A strain (FERM BP-1429) and artificial mutants thereof, which have the properties of forming and accumulating erythritol in a culture solution when aerobically cultured on a liquid culture medium containing an assimilable carbon source and an assimilable nitrogen source, and are capable of preparing erythritol by fermentation of sugars.
Claim 2
Dependent Claim
56 words
A biologically pure culture of Aureobasidium sp. SN-124A strain (FERM BP-1429) and artificial mutants thereof, which have the properties of forming and accumulating erythritol in a culture solution when aerobically cultured on a liquid culture medium containing an assimilable carbon source and an assimilable nitrogen source, and are capable of preparing erythritol by fermentation of sugars.
Claim 3
Dependent Claim
74 words
A biologically pure culture strain of an artificial mutant of Aureobasidium sp. SN-124A strain (BP-1429), which has the properties of forming and accumulating erythritol in a culture solution without any substantial occurrence of foams due to fermentation when aerobically cultured on a liquid medium containing an assimilable carbon source in an amount of at least 40% and an assimilable nitrogen source, produce hydrophilic cells, and is capable of preparing erythritol by fermentation of sugars.
Claim 4
Dependent Claim
74 words
A biologically pure culture strain of an artificial mutant of Aureobasidium sp. SN-124A strain (BP-1429), which has the properties of forming and accumulating erythritol in a culture solution without any substantial occurrence of foams due to fermentation when aerobically cultured on a liquid medium containing an assimilable carbon source in an amount of at least 40% and an assimilable nitrogen source, produce hydrophilic cells, and is capable of preparing erythritol by fermentation of sugars.
Claim 5
Dependent Claim
25 words
The biologically pure culture strain as defined in claim 1, in which said artificial mutant of Aureobasidium sp. SN-124A strain is Aureobasidium sp. SN-G42 strain
Claim 6
Dependent Claim
27 words
The biological pure culture strain as defined in claim 3, in which said artificial mutant of Aureobasidium sp. SN-124A strain is Aureobasidium sp. SN-γ96 strain (FERM BP-1431).