§
Luxuse
Patent Intelligence
Sign In
← Back to Patent Explorer
US5028424A Expired / Lapsed US ⏱️ 0.0 years remaining

Antibodies to receptor and antigen for natural killer and non-specific cytotoxic cells

Assignee: University of Georgia
•
Filing: 1987-10-16
•
Granted: 1991-07-02
•
Expires: 2007-10-16
📄 Original PDF 🌐 Google Patents 🔄 Sync Now

Abstract

Abstract The present invention encompasses a receptor molecule present on the surface of natural killer cells (NK cells) and non-specific cytotoxic cells (NCC) which is involved in non-specific lysis of target cells bearing an antigen recognized by the receptor molecule, an antigen common to the surface of cells recognized and lysed by natural killer cells, monoclonal and heterologous antibodies which bind to the receptor and target cell antigen(s) which are useful in their identification and purification, and methods for altering NK cell-mediated lysis of target cells. The monoclonal antibodies (mAbs) of the present invention were prepared by cell fusions between spleen cells from mice immunized with either non-specific cytotoxic cells (NCC) from catfish (anti-receptor antibodies) or NC-37 human lymphoblastoid B-cells, which are susceptible to lysis by NK cells, and myeloma cells. Eight distinct mAbs have been characterized, four directed against the NK/NCC target cell receptor and four against the NK/NC cell antigen. As used in the present invention "NK cells" will be used to include both natural killer cells of mammalian origin and non-specific cytotoxic cells of fish origin, unless specifically stated otherwise. Two of the mAbs directed against the receptor and two of the mAbs against the NK target cell antigen are able to significantly inhibit NK lysis at low mAb concentrations, as assessed by inhibition of cellular toxicity. Two of the mAbs directed against the receptor and two of the mAbs against the NK target cell antigen bind to the antigen(s) but do not inhibit lysis.

Inventors

  • 👤 Donald L. Evans

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-11-06 10:46
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/18; C07K16/28; A; A61; A61K; A61K38/00; Y; Y10; Y10S; Y10S530/00; Y10S530/863; Y10S530/864

Claims Analysis

Total Claims: 8
Claim 1
Independent Claim 109 words
A method for altering lysis of target cells by natural killer cells of mammalian or fish origin comprising providing an antibody to a receptor found as a liner in the presence of a reducing agent having a subunit with a molecular weight of about 38,000 daltons and a subunit with a molecular weight of about 43,000 daltons, on the surface of natural killer cells of mammalian and fish origin that is recognized by an antibody produced by a hybridoma selected from the group deposited with the ATCC on Oct. 16, 1987 and designated HB9572 and HB9573 and which is involved in non-MHC restricted, non-antigen specific cytolysis of target cells.
Claim 2
Independent Claim 109 words
A method for altering lysis of target cells by natural killer cells of mammalian or fish origin comprising providing an antibody to a receptor found as a liner in the presence of a reducing agent having a subunit with a molecular weight of about 38,000 daltons and a subunit with a molecular weight of about 43,000 daltons, on the surface of natural killer cells of mammalian and fish origin that is recognized by an antibody produced by a hybridoma selected from the group deposited with the ATCC on Oct. 16, 1987 and designated HB9572 and HB9573 and which is involved in non-MHC restricted, non-antigen specific cytolysis of target cells.
Claim 3
Dependent Claim 30 words
The method of claim 1 wherein the antibody is to a receptor recognizing an antigen on the surface of a target cell susceptible of lysis by the natural killer cells.
Claim 4
Dependent Claim 31 words
The method of claim 1 wherein said antibody is derived from a hybridoma produced by fusing a myeloma cell with spleen cells from an animal immunized with fish non-specific cytotoxic cells.
Claim 5
Dependent Claim 20 words
The method of claim 3 produced by a hybridoma selected from the group consisting of 5C6.10.4, 6D3.2.10, 2B2.4.9, and 6D3.4.4.
Claim 6
Dependent Claim 15 words
The method of claim 1 wherein lysis is inhibited by the addition of the antibody.
Claim 7
Independent Claim 117 words
A method for delivering a biologically active agent to natural killer cells or non-specific cytotoxic cells comprising providing an antibody to a receptor found as a non-disulfide linked heterodimer having a subunit with a molecular weight of about 38,000 daltons and a subunit with a molecular weight of about 43,000 dollars on the surface of natural killer cells of mammalian and fish origin that is recognized by an antibody produced by a hybridoma selected from the hybridomas deposited with the ATCC on Oct. 16, 1987 and designated HB9572 and HB9573 and which is involved in non-MHC restricted, non-antigen specific cytolysis of target cells, in combination with a biologically active agent and exposing the cells to the antibody.
Claim 8
Independent Claim 117 words
A method for delivering a biologically active agent to natural killer cells or non-specific cytotoxic cells comprising providing an antibody to a receptor found as a non-disulfide linked heterodimer having a subunit with a molecular weight of about 38,000 daltons and a subunit with a molecular weight of about 43,000 dollars on the surface of natural killer cells of mammalian and fish origin that is recognized by an antibody produced by a hybridoma selected from the hybridomas deposited with the ATCC on Oct. 16, 1987 and designated HB9572 and HB9573 and which is involved in non-MHC restricted, non-antigen specific cytolysis of target cells, in combination with a biologically active agent and exposing the cells to the antibody.

References Cited (Backward Citations)

US4550086A Cited
US4607007A Cited
US4713332A Cited
US4739045A Cited
US4734363A Cited
US4767700A Cited

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1994-07-01 1994-12-31 1995-07-02 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 1998-07-01 1998-12-31 1999-07-02 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 2002-07-01 2002-12-31 2003-07-02 $7700.00 Window Closed / Lapsed

Assigned Categories & Tags

No categories assigned to this patent yet.

Assign Category Tag