US4739045A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for the dissolution of nucleotides and polynucleotides
Assignee: Shell USA Inc
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Filing: 1985-03-05
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Granted: 1988-04-19
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Expires: 2005-03-05
Abstract
Abstract Process for the dissolution of nucleotides and polynucleotides in non-aqueous solvents and mixed non-aqueous solvent systems using macromolecular ligands, solutions thereby obtained and their use for the separation and purification of DNA, e.g. for RNA and protein, in the chemical synthesis of DNA, in the conversion of double stranded DNA into single stranded DNA, and in the chemical modification of DNA.
Inventors
- 👤 Barbara Odell
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 00:36
CPC Classifications
C; C07; C07H; C07H1/00; C07H1/06; C07H21/00
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
46 words
A process for the dissolution of DNA polynucleotides in a non-aqueous medium which consists of treating DNA polynucleotides with a non-aqueous medium in the presence of a macromolecular ligand or a mixture of macromolecular ligands to obtain a solution of solubilized DNA polynucleotides in the medium.
Claim 2
Dependent Claim
46 words
A process for the dissolution of DNA polynucleotides in a non-aqueous medium which consists of treating DNA polynucleotides with a non-aqueous medium in the presence of a macromolecular ligand or a mixture of macromolecular ligands to obtain a solution of solubilized DNA polynucleotides in the medium.
Claim 3
Dependent Claim
20 words
A process according to claim 1 in which the weight ratio of macromolecular ligand:polynucleotide is in the range of 1-500:1.
Claim 4
Dependent Claim
26 words
A process according to claim 2 in which the macromolecular ligand is selected from the group consisting of 18-crown-6, 15-crown-5, dibenzo-18-crown-6, dicyclohexyl-18-crown-6, 21-crown-7, and cryptand [222].
Claim 5
Dependent Claim
41 words
A process according to claim 1 in which the non-aqueous medium is a solvent selected from the group consisting of methanol, ethanol or dimethylsulphoxide or a mixture of these solvents with dichloromethane, chloroform, ethyl acetate, acetone, toluene, diethyl ether and tetrahydrofuran.
Claim 6
Dependent Claim
11 words
A process according to claim 4 wherein the solvent is methanol.
Claim 7
Dependent Claim
21 words
A process according to claim 1 in which the polynucleotides are in the form of the free acid or its salt.
Claim 8
Dependent Claim
24 words
A process according to claim 1 in which a substantial amount of the macromolecular ligands had been attached to organic or inorganic support materials.
Claim 9
Dependent Claim
45 words
A process in which double-stranded DNA is converted into single-stranded DNA which consists of treating double-stranded DNA with a non-aqueous medium in the presence of a macromolecular ligand or a mixture of macromolecular ligands and recovering the single-stranded DNA as a solution in the medium.
Claim 10
Dependent Claim
45 words
A process in which double-stranded DNA is converted into single-stranded DNA which consists of treating double-stranded DNA with a non-aqueous medium in the presence of a macromolecular ligand or a mixture of macromolecular ligands and recovering the single-stranded DNA as a solution in the medium.
References Cited (Backward Citations)
US3433782A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-04-19 | 1991-10-19 | 1992-04-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-04-19 | 1995-10-19 | 1996-04-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-04-19 | 1999-10-19 | 2000-04-19 | $7700.00 | Window Closed / Lapsed |