US4713332A
Expired / Lapsed
US
⏱️ 0.0 years remaining
T cell specific CDNA clone
Assignee: Princess Margaret Cancer Centre
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Filing: 1984-02-06
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Granted: 1987-12-15
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Expires: 2004-02-06
Abstract
Abstract The invention provides a nucleic acid having a sequence which encodes a polypeptide that is at least part of a T cell antigen receptor. This encoded sequence is about 936 nucleotides in length and preferably is a human T cell antigen receptor. The nucleic acid sequence of one embodiment of the invention is shown in FIG. 3. The nucleic acid sequence may be used as a probe to determine whether an unknown cell, e.g., a tumor cell, is a T cell. Polypeptides encoded by the nucleic acid sequence include about 312 amino acids and are at least part of a T cell antigen receptor. They include at least one sequence which over 21 contiguous amino acids has greater than about 35% homology with mouse and human immunoglobin λ light chains. Antibody to the polypeptide may be prepared and used to identify T cell antigen receptor and to determine whether an unknown cell, e.g., a tumor cell, is a T cell.
Inventors
- 👤 Tak W. Mak
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 11:35
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/566; C; C07; C07K; C07K14/00; C07K14/435; C07K14/705; C07K14/70503; C07K14/7051; C07K14/82; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6876; C12Q1/6881; C12Q2600/00; C12Q2600/158; Y; Y10; Y10S; Y10S930/00; Y10S930/01; Y10S930/30
Claims Analysis
Total Claims: 26
Claim 1
Independent Claim
30 words
An isolated nucleic acid sequence encoding a polypeptide which is at least part of the beta chain of a human T cell antigen receptor comprising at least about 936 nucleotides.
Claim 2
Dependent Claim
30 words
An isolated nucleic acid sequence encoding a polypeptide which is at least part of the beta chain of a human T cell antigen receptor comprising at least about 936 nucleotides.
Claim 3
Dependent Claim
12 words
A nucleic acid of claim 1, wherein the nucleic acid is DNA.
Claim 4
Dependent Claim
13 words
A nucleic acid of claim 2, wherein the nucleic acid is a cDNA.
Claim 5
Dependent Claim
12 words
A nucleic acid of claim 1, wherein the nucleic acid is RNA.
Claim 6
Dependent Claim
29 words
A nucleic acid of claim 2 containing at the 5' end at least about a 174 nucleotide sequence identical to the sequence of YT35 at positions 973 to 800.
Claim 7
Dependent Claim
29 words
A nucleic acid of claim 4 containing at the 3' end at least about a 174 nucleotide sequence complementary to the sequence of YT35 at positions 973 to 800.
Claim 8
Dependent Claim
11 words
A nucleic acid of claim 2 having the sequence of YT35.
Claim 9
Dependent Claim
20 words
A substantially pure DNA sequence being approximately 60% or more homologous with the clone YT35 as measured by hybridization therewith.
Claim 10
Dependent Claim
20 words
A substantially pure DNA sequence being approximately 60% or more homologous with the clone YT35 as measured by hybridization therewith.
Claim 11
Dependent Claim
11 words
A nucleic acid of claim 1 labeled with a detectable marker.
Claim 12
Dependent Claim
11 words
A nucleic acid of claim 9, wherein the marker is radioactive.
Claim 13
Dependent Claim
11 words
A nucleic acid of claim 9, wherein the marker is fluorescent.
Claim 14
Dependent Claim
11 words
A nucleic acid of claim 9, wherein the marker is biotinylated.
Claim 15
Dependent Claim
75 words
A method of preparing the cDNA of claim 3 which comprises obtaining mRNA from a human T cell, preparing cDNA complementary to the mRNA, inserting the cDNA into an appropriate cloning vehicle, introducing the cloning vehicle into a suitable host, culturing the resulting host under appropriate conditions permitting production of multiple copies of the cDNA, recovering the cDNA so produced and screening the cDNA to determine whether said cDNA is expressed only in T cells.
Claim 16
Dependent Claim
13 words
A method of claim 13, wherein the T cell is an MOLT-3 cell.
Claim 17
Dependent Claim
12 words
A method of claim 13, wherein the T cell is a thymocyte.
Claim 18
Dependent Claim
11 words
A method of claim 13, wherein the cloning vehicle is pBR322.
Claim 19
Dependent Claim
12 words
A method of claim 13, wherein the suitable host is E. coli.
Claim 20
Dependent Claim
10 words
A cloning vehicle comprising the nucleic acid of claim 1.
Claim 21
Dependent Claim
10 words
A cloning vehicle comprising the nucleic acid of claim 3.
Claim 22
Dependent Claim
10 words
A cloning vehicle comprising the nucleic acid of claim 8.
Claim 23
Dependent Claim
10 words
A host cell comprising the cloning vehicle of claim 18.
Claim 24
Dependent Claim
9 words
A microorganism comprising the cloning vehicle of claim 18.
Claim 25
Dependent Claim
9 words
A microorganism comprising the cloning vehicle of claim 20.
Claim 26
Dependent Claim
49 words
A method of producing a polypeptide which is at least part of a human T cell antigen receptor which comprises introducing the cloning vehicle of claim 18 into a suitable host, culturing the resulting host under appropriate conditions permitting expression of the polypeptide and recovering the polypeptide so expressed.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-12-14 | 1991-06-15 | 1991-12-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-12-14 | 1995-06-15 | 1995-12-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-12-14 | 1999-06-15 | 1999-12-15 | $7700.00 | Window Closed / Lapsed |