Patent US5018209A successfully refreshed from Google Patents.
US5018209A
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US
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Analysis method and apparatus for biological specimens
Assignee: Cell Analysis Systems Inc
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Filing: 1986-11-04
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Granted: 1991-05-21
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Expires: 2006-11-04
Abstract
Abstract A method and apparatus are provided for selecting and analyzing a subpopulation of cells or cell objects for a certain parameter such as DNA, estrogen, and then measuring the selected cells. The observer in real time views a field of cells and then gates for selection based on the morphological criteria those cells that have the visual parameter such as colored DNA or colored antigen into a subpopulation that is to be measured. The selected cells are examined by digital image processing and are measured for a parameter such as a true actual measurement of DNA in picograms. A quantitation of the measured parameter is generated and provided.
Inventors
- 👤 James W. Bacus
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 22:21
CPC Classifications
G; G06; G06V; G06V20/00; G06V20/60; G06V20/69; G06V20/693; G01; G01N; G01N15/00; G01N15/10; G01N15/14; G01N15/1468; G01N15/1429; G01N15/1433; G01N15/1012; G01N2015/1014; G01N21/00; G01N21/62; G01N21/63; G01N21/64; G01N21/645; G01N21/6456; G01N21/6458; G01N2201/00; G01N2201/12; G01N2201/127
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
186 words
An interactive method for selecting a subpopulation of cell objects from a population of cell objects and for measuring a subpopulation of malignant cell objects for a given measured parameter with a digital image processing means, said method comprising the steps of: providing in real time an enlarged view of a field containing a plurality of malignant cell objects from the population of cell objects to a user-observer through the digital image processing means, visually selecting which of the plurality of cell objects have visual parameters identifying them for being placed into a selected subpopulation of malignant cell objects to be measured and rejecting from the subpopulation those cells objects being viewed and lacking the visual parameters, measuring only the selected malignant cell objects in the selected subpopulation for the measured parameter with a digital image processing means, and generating with a digital image processing means a quantitation based on the measured parameter for the selected malignant cell object subpopulation, including the generation of a parameter distribution of the measured parameter for the malignant cells showing a first peak and/or second peak for the selected subpopulation.
Claim 2
Dependent Claim
186 words
An interactive method for selecting a subpopulation of cell objects from a population of cell objects and for measuring a subpopulation of malignant cell objects for a given measured parameter with a digital image processing means, said method comprising the steps of: providing in real time an enlarged view of a field containing a plurality of malignant cell objects from the population of cell objects to a user-observer through the digital image processing means, visually selecting which of the plurality of cell objects have visual parameters identifying them for being placed into a selected subpopulation of malignant cell objects to be measured and rejecting from the subpopulation those cells objects being viewed and lacking the visual parameters, measuring only the selected malignant cell objects in the selected subpopulation for the measured parameter with a digital image processing means, and generating with a digital image processing means a quantitation based on the measured parameter for the selected malignant cell object subpopulation, including the generation of a parameter distribution of the measured parameter for the malignant cells showing a first peak and/or second peak for the selected subpopulation.
Claim 3
Dependent Claim
56 words
A method in accordance with claim 1 in which the step of selecting includes the step of gating the malignant cell objects into the subpopulation and in which the measuring step includes the measurement of DNA in the gated cell objects into a normal cell object subpopulation and into one of several malignant cell object subpopulations.
Claim 4
Dependent Claim
34 words
A method in accordance with claim 1 in which the classifying step includes the user classifying the cell objects into a normal cell object subpopulation and into one of several abnormal cell object subpopulations.
Claim 5
Dependent Claim
26 words
A method in accordance with claim 1 in which said subpopulation of the cell objects is a small fraction of the total population of cell objects.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-05-20 | 1994-11-19 | 1995-05-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-05-20 | 1998-11-19 | 1999-05-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-05-20 | 2002-11-19 | 2003-05-21 | $7700.00 | Window Closed / Lapsed |