US5013657A
Expired / Lapsed
US
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Subtilisin mutations
Assignee: Genex Corp
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Filing: 1988-04-12
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Granted: 1991-05-07
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Expires: 2008-04-12
Abstract
Abstract The invention relates to subtilisin enzymes which have been modified by mutating a nucleotide sequence (gene) coding for the subtilisin. The modified subtilisin enzymes have enhanced thermal stability.
Inventors
- 👤 Philip N. Bryan
- 👤 Michael W. Pantoliano
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 22:07
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/52; C12N9/54
Claims Analysis
Total Claims: 3
Claim 1
Independent Claim
22 words
A mutant subtilisin gene encoding a thermally stable subtilisin BPN' comprising an amino acid substitution of cysteine at amino acid position 206.
Claim 2
Dependent Claim
22 words
A mutant subtilisin gene encoding a thermally stable subtilisin BPN' comprising an amino acid substitution of cysteine at amino acid position 206.
Claim 3
Dependent Claim
59 words
A method of increasing the thermal stability of subtilisin comprising mutagenizing DNA encoding subtilisin BPN' using oligonucleotide matagenesis to substitute DNA encoding glutamine at amino acid position 206 with DNa encoding a cysteine residue in order to produce the mutant subtilisin gene of claim 1 and expressing said mutant gene to make a mutated subtilisin with increased thermal stability.
References Cited (Backward Citations)
US4760025A
Cited
US4749511A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-05-06 | 1994-11-05 | 1995-05-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-05-06 | 1998-11-05 | 1999-05-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-05-06 | 2002-11-05 | 2003-05-07 | $7700.00 | Window Closed / Lapsed |