US4760025A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Modified enzymes and methods for making same
Assignee: Genencor Inc
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Filing: 1984-05-29
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Granted: 1988-07-26
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Expires: 2004-05-29
Abstract
Abstract A cloned subtilisin gene has been modified at specific sites to cause amino acid substitutions at certain spots in the enzyme. The modified enzyme, preferably produced by Bacillus, is useful in combination with detergents.
Inventors
- 👤 David A. Estell
- 👤 James A. Wells
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 15:58
CPC Classifications
C; C12; C12N; C12N1/00; C12N1/20; C12N1/205; C11; C11D; C11D3/00; C11D3/16; C11D3/38; C11D3/386; C12N15/00; C12N15/09; C12N15/10; C12N15/102; C12N15/63; C12N15/74; C12N15/75; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/52; C12N9/54; C12R; C12R2001/00; C12R2001/01; C12R2001/07
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
30 words
A subtilisin enzyme having a different amino acid at a site +32, +155, +104, +222, +166, +64, +33, +169, +189, +217, or +157 than subtilisin naturally produced by Bacillus amyloliquefaciens.
Claim 2
Dependent Claim
30 words
A subtilisin enzyme having a different amino acid at a site +32, +155, +104, +222, +166, +64, +33, +169, +189, +217, or +157 than subtilisin naturally produced by Bacillus amyloliquefaciens.
Claim 3
Dependent Claim
14 words
A composition comprising an enzyme according to claim 1 in combination with a detergent.
Claim 4
Dependent Claim
25 words
A composition according to claim 2 wherein the detergent is a linear alkyl benzene sulfonate, alkyl benzene sulfate, sulfated linear alcohol, or ethoxylated linear alcohol.
References Cited (Backward Citations)
US3985686A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-07-26 | 1992-01-25 | 1992-07-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-07-26 | 1996-01-25 | 1996-07-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-07-26 | 2000-01-25 | 2000-07-26 | $7700.00 | Window Closed / Lapsed |