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US4985543A
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Lectins and antiretroviral drugs containing the lectins as active ingredient
Assignee: Kureha Corp
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Filing: 1988-06-17
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Granted: 1991-01-15
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Expires: 2008-06-17
Abstract
Abstract Disclosed herein are lectins extracted form a plant of the class Dicotyledoneae and an antiretoviral drug comprising as an active ingredient an effective amount of the lectins.
Inventors
- 👤 Norifumi Sugita
- 👤 Koichi Niimura
- 👤 Yoshiharu Oguchi
- 👤 Kunitaka Hirose
- 👤 Kenichi Matsunaga
- 👤 Minoru Oohara
- 👤 Shigeaki Muto
- 👤 Junji Kakuchi
- 👤 Takao Furusho
- 👤 Chikao Yoshikumi
- 👤 Masaaki Takahashi
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 15:01
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/415; C07K14/42; A; A61; A61K; A61K36/00; A61K36/18; A61K36/185; A61K36/36; A61K36/47; A61K36/48; A61P; A61P31/00; A61P31/12
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
189 words
A lectin obtained from a plant selected from: (1) Lotus tetragonolobus and Ulex europeus; (2) Abrus precatorius, Arachis hypogaea, Bandeirea simplifolia, Banhinia purpunea, Calpuria aegggptiana, Fomes formentarius, Glycine max, Maackia amurensis, Phaseolus lunatas, Ricinus communis, Sophora japonica, Wistaria floribunda and Vicia cracca; and (3) Canavalia ensiformis, Lens eulinaria, Pisum satiuvm, Vicia faba, Jack bean, Cytisus sessilifolius, Laburnus alpinum, Cerastrium fomentosus and Ulex europeus, each of (1), (2) and (3) being in the Makela classification, said lectin having the following properties: (a) positive results when subjected to the α-naphthol-sulfuric acid, indole-sulfuric acid, anthrone-sulfuric acid or phenol-sulfuric acid reaction and, after hydrochloric acid hydrolysis, the Lowry-Folin process or the ninhydrin reaction; (b) elementary analysis: 20-55% carbon, 3-9% hydrogen and 0.1-16% nitrogen; (c) a pH of 6.0-7.0; (d) the sugar component, when present, comprises at least glucose and N-acetyl-glucosamine and the protein component comprises at least glutamic acid, aspartic acid and lysine; (e) infrared absorption spectrum peaks at 3,600-3,200 cm -1 ; (f) a molecular weight of 10 3 -3×10 6 as measured by gel filtration chromatography; and (g) soluble in water and aqueous solvent, and insoluble in chloroform, benzene and ether.
Claim 2
Dependent Claim
189 words
A lectin obtained from a plant selected from: (1) Lotus tetragonolobus and Ulex europeus; (2) Abrus precatorius, Arachis hypogaea, Bandeirea simplifolia, Banhinia purpunea, Calpuria aegggptiana, Fomes formentarius, Glycine max, Maackia amurensis, Phaseolus lunatas, Ricinus communis, Sophora japonica, Wistaria floribunda and Vicia cracca; and (3) Canavalia ensiformis, Lens eulinaria, Pisum satiuvm, Vicia faba, Jack bean, Cytisus sessilifolius, Laburnus alpinum, Cerastrium fomentosus and Ulex europeus, each of (1), (2) and (3) being in the Makela classification, said lectin having the following properties: (a) positive results when subjected to the α-naphthol-sulfuric acid, indole-sulfuric acid, anthrone-sulfuric acid or phenol-sulfuric acid reaction and, after hydrochloric acid hydrolysis, the Lowry-Folin process or the ninhydrin reaction; (b) elementary analysis: 20-55% carbon, 3-9% hydrogen and 0.1-16% nitrogen; (c) a pH of 6.0-7.0; (d) the sugar component, when present, comprises at least glucose and N-acetyl-glucosamine and the protein component comprises at least glutamic acid, aspartic acid and lysine; (e) infrared absorption spectrum peaks at 3,600-3,200 cm -1 ; (f) a molecular weight of 10 3 -3×10 6 as measured by gel filtration chromatography; and (g) soluble in water and aqueous solvent, and insoluble in chloroform, benzene and ether.
Claim 3
Dependent Claim
31 words
The lectin according to claim 1, which is obtained by extracting said plant with an aqueous solvent at 4°-150° C. for 20 minutes to 20 hours, and thereafter refining the extract.
Claim 4
Dependent Claim
23 words
The lectin according to claim 2, wherein said aqueous solvent is an aqueous solution of a water-soluble organic solvent, acid, base or salt.
Claim 5
Dependent Claim
23 words
The lectin according to claim 3, wherein said aqueous solution is an aqueous solution of ammonia, sodium hydroxide, potassium hydroxide or sodium carbonate.
Claim 6
Dependent Claim
28 words
The lectin according to claim 2, wherein the extract is further refined by salting-out, affinity chromatography, dialysis, ultrafiltration, reverse osmosis, gel filtration, or precipitation with an organic solvent.
References Cited (Backward Citations)
US4699787A
Cited
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