US4973394A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Immobilized valinomycin molecule for K+ sensor
Assignee: SRI International Inc
•
Filing: 1988-09-02
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Granted: 1990-11-27
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Expires: 2008-09-02
Abstract
Abstract The present invention provides a stabilized potassium ion-selective membrane comprising lysine-substituted valinomycin covalently bound to a polymeric substrate. The membrane of the invention exhibits improved stability and is used either on ISEs or on solid-state coated wire or semiconductor devices.
Inventors
- 👤 Pepi Ross
- 👤 Allan J. Johnston
- 👤 Amrit K. Judd
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 20:28
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/28; G01N27/30; G01N27/333; G01N27/3335; Y; Y10; Y10S; Y10S435/00; Y10S435/817; Y10S436/00; Y10S436/806
Claims Analysis
Total Claims: 24
Claim 1
Independent Claim
17 words
A stabilized potassium ion-selective membrane comprising lysine-substituted valinomycin covalently bonded to an active site-containing hydrophobic polymer substrate.
Claim 2
Dependent Claim
17 words
A stabilized potassium ion-selective membrane comprising lysine-substituted valinomycin covalently bonded to an active site-containing hydrophobic polymer substrate.
Claim 3
Dependent Claim
23 words
The membrane of claim 1 wherein the active site is selected from the group consisting of carboxylic acid, carbonyl, hydroxyl, and amide groups.
Claim 4
Dependent Claim
20 words
The membrane of claim 2 wherein the hydrophobic polymer substrate is fabricated to form a plasticized, high impedance, conducting membrane.
Claim 5
Dependent Claim
12 words
The membrane of claim 3 wherein the hydrophobic polymer comprises polyvinyl chloride.
Claim 6
Dependent Claim
11 words
The membrane of claim 2 wherein the membrane is liquid impermeable.
Claim 7
Dependent Claim
35 words
The membrane of claim 1 wherein the valinomycin is covalently bonded to the polymer substrate through an amide bond formed from the amine of the lysine substituent and a carboxylic acid group of the substrate.
Claim 8
Dependent Claim
22 words
The membrane of claim 1 wherein a spacer arm is inserted between the reactive amine of the lysine residue and the substrate.
Claim 9
Dependent Claim
23 words
The membrane of claim 7 wherein the spacer arm is an organic spacer arm from about 1 to about 20 atoms in length.
Claim 10
Dependent Claim
37 words
The membrane of claim 7 wherein the spacer arm is covalently bonded at one end to the primary amine of the lysine residue and at the other end to a carboxylic acid group of a carboxylated polymer.
Claim 11
Dependent Claim
35 words
The membrane of claim 9 wherein the reactive spacer arm comprises a linear diaminoalkane of the formula NH 2 (CH 2 ) n NH 2 , wherein n is an integer from 1 to 10.
Claim 12
Dependent Claim
10 words
The membrane of claim 1 wherein the membrane is insulating.
Claim 13
Dependent Claim
47 words
In a potassium ion selective sensor wherein potassium ion selectivity is imparted by the selective association of potassium ion with a valinomycin species, the improvement comprising employing as the valinomycin species, a lysine substituted valinomycin covalently bonded via the amine group of the lysine to a polymer.
Claim 14
Dependent Claim
47 words
In a potassium ion selective sensor wherein potassium ion selectivity is imparted by the selective association of potassium ion with a valinomycin species, the improvement comprising employing as the valinomycin species, a lysine substituted valinomycin covalently bonded via the amine group of the lysine to a polymer.
Claim 15
Dependent Claim
18 words
The sensor of claim 12 wherein the polymerbound valinomycin is in the form of a liquid permeable membrane.
Claim 16
Dependent Claim
58 words
The sensor of claim 13 configured as a potassium ion-selective electrode comprising: an internal reference element having a predetermined concentration of salt solution; and in physical contact with said reference element, a hydrophobic potassium ion-selective membrane comprising a lysine derivative of valinomycin covalently bonded through the amine group of the lysine to an active site on the polymer.
Claim 17
Dependent Claim
19 words
The sensor of claim 12 wherein the polymer bound valinomycin is in the form of a liquid impermeable membrane.
Claim 18
Dependent Claim
10 words
The sensor of claim 15 wherein the membrane is insulating.
Claim 19
Dependent Claim
12 words
The sensor of claim 15 configured as a solid state semiconductor device.
Claim 20
Dependent Claim
29 words
The sensor of claim 17 wherein the solid state semiconductor device is a field effect transistor with said liquid impermeable membrane being an insulating membrane over its gate region.
Claim 21
Dependent Claim
17 words
The sensor of claim 18 further comprising at least one silicon layer adjacent to said insulating membrane.
Claim 22
Dependent Claim
22 words
The sensor of claim 17 wherein the membrane is formed using glow discharge polymerization of an organic monomer with a carrier gas.
Claim 23
Dependent Claim
21 words
The sensor of claim 20 wherein the organic monomer is selected from the group consisting of 1,3-perfluorodimethylcyclohexane, acetylene, paralene and styrene.
Claim 24
Dependent Claim
22 words
The sensor of claim 20 wherein the carrier gas comprises a member selected from the group consisting of nitrogen, oxygen and argon.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-11-26 | 1994-05-28 | 1994-11-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-11-26 | 1998-05-28 | 1998-11-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-11-26 | 2002-05-28 | 2002-11-27 | $7700.00 | Window Closed / Lapsed |