US4971677A
Expired / Lapsed
US
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Fluorescence detection type electrophoresis apparatus
Assignee: Hitachi Ltd
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Filing: 1989-02-15
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Granted: 1990-11-20
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Expires: 2009-02-15
Abstract
Abstract In an electrophoresis apparatus for separating and detecting a sample of DNA or RNA labelled with fluorescence, use is made of a gel plate having a polyacrylamide concentration of 2 to 6% in order to greatly shorten the time required for the measurement.
Inventors
- 👤 Hideki Kambara
- 👤 Yoshiko Katayama
- 👤 Tetsuo Nishikawa
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 15:54
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44717; G01N27/44721
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
87 words
In a fluorescence detection type electrophoresis apparatus for determining base sequence of a sample in real time comprising an electrophoresis separation device for electrophoretically separating said sample labelled with fluorescence, an excitation light source for exciting said sample, and a detection means for detecting the fluorescence emitted by said sample that is excited, the improvement wherein use is made of a gel plate having a polyacrylamide concentration of 2 to 6% in terms of percentage of weight/volume (g/ml) of total monomer concentration as said electrophoresis separation device.
Claim 2
Dependent Claim
87 words
In a fluorescence detection type electrophoresis apparatus for determining base sequence of a sample in real time comprising an electrophoresis separation device for electrophoretically separating said sample labelled with fluorescence, an excitation light source for exciting said sample, and a detection means for detecting the fluorescence emitted by said sample that is excited, the improvement wherein use is made of a gel plate having a polyacrylamide concentration of 2 to 6% in terms of percentage of weight/volume (g/ml) of total monomer concentration as said electrophoresis separation device.
Claim 3
Dependent Claim
19 words
A fluorescence detection type electrophoresis apparatus according to claim 1, wherein said polyacrylamide concentration is from 3.2 to 4.3%.
Claim 4
Dependent Claim
19 words
A fluorescence detection type electrophoresis apparatus according to claim 1, wherein said polyacrylamide concentration is from 2.6 to 3.2%.
Claim 5
Dependent Claim
91 words
In a fluorescence detection type electrophoresis apparatus for determining base sequence of a sample of DNA or RNA in real time comprising an electrophoresis separation device for electrophoretically separating said sample labelled with fluorescence, an excitation light source for exciting said sample, and a detection means for detecting the fluorescence emitted by said sample that is excited, the improvement wherein use is made of a gel plate having a polyacrylamide concentration of 2 to 6% in terms of percentage of weight/volume (g/ml) of total monomer concentration as said electrophoresis separation device.
Claim 6
Dependent Claim
91 words
In a fluorescence detection type electrophoresis apparatus for determining base sequence of a sample of DNA or RNA in real time comprising an electrophoresis separation device for electrophoretically separating said sample labelled with fluorescence, an excitation light source for exciting said sample, and a detection means for detecting the fluorescence emitted by said sample that is excited, the improvement wherein use is made of a gel plate having a polyacrylamide concentration of 2 to 6% in terms of percentage of weight/volume (g/ml) of total monomer concentration as said electrophoresis separation device.
Claim 7
Dependent Claim
148 words
A fluorescence detection type electrophoresis apparatus for determining base sequence of a sample of DNA or RNA labelled with fluorescence in real time comprising: an electrophoresis separation device comprising a gel plate holding an electrophoresis gel for electrophoretically separating said sample by migration of said sample from a first end of said gel towards a second end of said gel, wherein said gel contains polyacrylamide at a concentration of 2 to 6% in terms of percentage of weight/volume (g/ml) of total monomer concentration; an excitation light source directed on said gel at a position separated by a predetermined distance from said first end for exciting said sample; and a detection means for detecting fluorescence emitted by said sample as it is excited and passes through said position; whereby said base sequence of said sample can be determined by separating and detecting fragments of said sample in real time.
Claim 8
Dependent Claim
148 words
A fluorescence detection type electrophoresis apparatus for determining base sequence of a sample of DNA or RNA labelled with fluorescence in real time comprising: an electrophoresis separation device comprising a gel plate holding an electrophoresis gel for electrophoretically separating said sample by migration of said sample from a first end of said gel towards a second end of said gel, wherein said gel contains polyacrylamide at a concentration of 2 to 6% in terms of percentage of weight/volume (g/ml) of total monomer concentration; an excitation light source directed on said gel at a position separated by a predetermined distance from said first end for exciting said sample; and a detection means for detecting fluorescence emitted by said sample as it is excited and passes through said position; whereby said base sequence of said sample can be determined by separating and detecting fragments of said sample in real time.
Claim 9
Dependent Claim
87 words
A fluorescence detection type electrophoresis apparatus for determining base sequence of a sample in real time comprising an electrophoresis separation device for electrophoretically separating said sample labelled with fluorescence, and excitation light source for exciting said sample, and a detection means for detecting the fluorescence emitted by said sample that is excited, the improvement wherein use is made of a gel plate having a polyacrylamide concentration of from 4.3 to 6.2% in terms of percentage of weight/volume (g/ml) of total monomer concentration as said electrophoresis separation device.
Claim 10
Dependent Claim
87 words
A fluorescence detection type electrophoresis apparatus for determining base sequence of a sample in real time comprising an electrophoresis separation device for electrophoretically separating said sample labelled with fluorescence, and excitation light source for exciting said sample, and a detection means for detecting the fluorescence emitted by said sample that is excited, the improvement wherein use is made of a gel plate having a polyacrylamide concentration of from 4.3 to 6.2% in terms of percentage of weight/volume (g/ml) of total monomer concentration as said electrophoresis separation device.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-11-19 | 1994-05-21 | 1994-11-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-11-19 | 1998-05-21 | 1998-11-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-11-19 | 2002-05-21 | 2002-11-20 | $7700.00 | Window Closed / Lapsed |