US4123343A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Purification of glycoproteins and immunization therewith
Assignee: Wyeth LLC
•
Filing: 1977-06-14
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Granted: 1978-10-31
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Expires: 1997-06-14
Abstract
Abstract {PG,1 A method is disclosed for the purification of the beta subunit of human chorionic gonadotrophin. Also disclosed is an antiserum composition and method of producing same using spleen immunization to obtain antiserum compositions having high antibody titers.
Inventors
- 👤 John Krupey
- 👤 Ewald F. Welchner
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 16:59
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; G01N33/76; A; A61; A61K; A61K39/00; A61K39/0005; A61K39/0006; C; C07; C07K; C07K14/00; C07K14/435; C07K14/575; C07K14/59; C07K16/00; C07K16/18; C07K16/26
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
88 words
A method for purifying the beta subunit of human chorionic gonatrophin which comprises subjecting the β-hCG subunit to polyacrylamide gel electrophoresis in a tube containing an upper gel and a lower gel suspended in an electrophoretic apparatus having upper and lower buffer chambers wherein both the lower gel and the upper chamber buffer contain a fluorescent probe consisting of the magnesium salt of 8-anilino-1-naphthalene-sulfonic acid terminating the electrophoresis, and cutting out the fluorescent boundaries corresponding to the β-hCG subunit located at the most anodic portion of the gel.
Claim 2
Independent Claim
88 words
A method for purifying the beta subunit of human chorionic gonatrophin which comprises subjecting the β-hCG subunit to polyacrylamide gel electrophoresis in a tube containing an upper gel and a lower gel suspended in an electrophoretic apparatus having upper and lower buffer chambers wherein both the lower gel and the upper chamber buffer contain a fluorescent probe consisting of the magnesium salt of 8-anilino-1-naphthalene-sulfonic acid terminating the electrophoresis, and cutting out the fluorescent boundaries corresponding to the β-hCG subunit located at the most anodic portion of the gel.
Claim 3
Dependent Claim
14 words
The method of claim 1, wherein said lower gel contains about 5.4% polymerized acrylamide.
Claim 4
Dependent Claim
25 words
The method of claim 2, wherein the concentration of the fluorescent probe in both the lower gel and upper buffer is about 0.5 milligram percent.
Claim 5
Dependent Claim
14 words
The beta subunit of human chorionic gonadotrophin obtained by the method of claim 1.
Claim 6
Independent Claim
73 words
A method for purifying a glycoprotein antigen which comprises subjecting said antigen to polyacrylamide gel electrophoresis in a tube containing an upper gel and a lower gel suspended in an electrophoretic apparatus having upper and lower buffer chambers wherein both the lower gel and the upper chamber buffer contain a fluorescent probe consisting of the magnesium salt of 8-anilino-1-naphthalene-sulfonic acid terminating the electorphoresis, and cutting out the fluorescent boundaries corresponding to said antigens.
Claim 7
Independent Claim
73 words
A method for purifying a glycoprotein antigen which comprises subjecting said antigen to polyacrylamide gel electrophoresis in a tube containing an upper gel and a lower gel suspended in an electrophoretic apparatus having upper and lower buffer chambers wherein both the lower gel and the upper chamber buffer contain a fluorescent probe consisting of the magnesium salt of 8-anilino-1-naphthalene-sulfonic acid terminating the electorphoresis, and cutting out the fluorescent boundaries corresponding to said antigens.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-10-30 | 1982-05-01 | 1982-10-31 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-10-30 | 1986-05-01 | 1986-10-31 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-10-30 | 1990-05-01 | 1990-10-31 | $7700.00 | Window Closed / Lapsed |