Patent US4965186A successfully refreshed from Google Patents.
US4965186A
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US
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Method for low-temperature preservation of spermatozoa
Assignee: INSTITUT PROBLEM KRIOBILOGII I KRIOMEDITSINY
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Filing: 1988-06-21
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Granted: 1990-10-23
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Expires: 2008-06-21
Abstract
Abstract The method resides in cooling the spermatozoa in a high thermal-diffusive powdered material that has preliminarily been cooled down to the refrigerant temperature. Cooling is carried out in a medium of a cryopreservative, containing a salt buffer, an antishock component, and a cryoprotector. The cryoprotector is taken in a concentration high enough for vitrification to develop in the spermatozoa. An example of the high thermal diffusive powdered material is alumina.
Inventors
- 👤 Valentin I. Grischenko
- 👤 Jury V. Kalugin
- 👤 Jury S. Paraschuk
- 👤 Nina A. Luchko
- 👤 Elena N. Chernysh
- 👤 Valery F. Tarasov
- 👤 Sergei E. Galchenko
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 04:37
CPC Classifications
A; A01; A01N; A01N1/00; A01N1/10; A01N1/12; A01N1/122; A01N1/125; A61; A61K; A61K35/00; A61K35/12; A61K35/48; A61K35/52
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
85 words
A method for cooling spermatozoa to the temperature of a refrigerant in which they are to be stored comprising: adding said spermatozoa to a cryopreservative; said cryopreservative comprising a salt buffer, an antishock component, and a cryoprotector, placing said spermatozoa in said cryopreservative into a container made of food packing foil; introducing said container with said spermatozoa into a thermal diffusive powdered material; said powdered material having been cooled to the temperature of the refrigerant; said powdered material being alumina (Al 2 O 3 ).
Claim 2
Independent Claim
85 words
A method for cooling spermatozoa to the temperature of a refrigerant in which they are to be stored comprising: adding said spermatozoa to a cryopreservative; said cryopreservative comprising a salt buffer, an antishock component, and a cryoprotector, placing said spermatozoa in said cryopreservative into a container made of food packing foil; introducing said container with said spermatozoa into a thermal diffusive powdered material; said powdered material having been cooled to the temperature of the refrigerant; said powdered material being alumina (Al 2 O 3 ).
Claim 3
Dependent Claim
21 words
A method as claimed in claim 1, wherein the cryoprotector is used in a concentration of from 1.5 to 2.5 percent.
Claim 4
Dependent Claim
13 words
A method as claimed in claim 1, wherein the cryopreservative contains choline chloride.
Claim 5
Dependent Claim
18 words
A method as claimed in claim 3, wherein choline chloride is taken in a concentration of 2 percent.
Claim 6
Dependent Claim
13 words
A method as in claim 1, wherein the antishock component is egg yolk.
References Cited (Backward Citations)
US3185623A
Cited
US3791384A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-10-22 | 1994-04-23 | 1994-10-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-10-22 | 1998-04-23 | 1998-10-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-10-22 | 2002-04-23 | 2002-10-23 | $7700.00 | Window Closed / Lapsed |