US3791384A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Artificial insemination of sows
Assignee: Individual
•
Filing: 1972-05-11
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Granted: 1974-02-12
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Expires: 1992-05-11
Abstract
Abstract A method of preserving boar sperm by deep-freezing thawing and introduction into the uterus of the sow, in which method the ejaculate after being collected is centrifuged, and the sediment is diluted, frozen to a temperature of -180* to -200*C, at the time of use is thawed by being placed in such a quantity of diluent warmed to 40* to 50* C that a dilution of the sperm which is appropriate for effective bathing of the uterus is achieved, and the diluted fluid is introduced into the uterus. The ejaculate is inactivated after being collected and before being centrifuged, and after being thawed the sperm solution has added to it a solution which mobilizes the spermatozoa.
Inventors
- 👤 L Richter
- 👤 A Liedicke
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 18:43
CPC Classifications
A; A61; A61D; A61D17/00; A01; A01K; A01K29/00
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
96 words
The method of impregnating a sow comprising the steps of immobilizing the spermatozoa of boar semen with a first diluting spermatozoa inactivating solution, centrifuging the diluted semen to produce a sperm sediment, diluting the sperm sediment with a freezing diluent, slowing cooling the resultant sperm solution to approximately 5* C, freezing and storing the cooled sperm solution at a temperature of approximately -180* C to -200* C, thawing the frozen sperm solution when needed, mixing the thawed sperm solution with a spermatozoa activating solution and utilizing said activated sperm solution within the uterus of a sow.
Claim 2
Independent Claim
96 words
The method of impregnating a sow comprising the steps of immobilizing the spermatozoa of boar semen with a first diluting spermatozoa inactivating solution, centrifuging the diluted semen to produce a sperm sediment, diluting the sperm sediment with a freezing diluent, slowing cooling the resultant sperm solution to approximately 5* C, freezing and storing the cooled sperm solution at a temperature of approximately -180* C to -200* C, thawing the frozen sperm solution when needed, mixing the thawed sperm solution with a spermatozoa activating solution and utilizing said activated sperm solution within the uterus of a sow.
Claim 3
Dependent Claim
26 words
In the method of impregnating a sow as in claim 1 wherein said first diluting solution and said freezing diluent contain glucose and a buffer system.
Claim 4
Dependent Claim
74 words
In the method of impregnating a sow as in claim 1 wherein the thawing of the frozen sperm solution, mixing with an activating solution and utilization within the uterus of a sow comprises the steps of thawing the frozen sperm solution within a spermatozoa diluting solution, introducing the thawed diluted solution within the uterus of a sow, and introducing a spermatozoa activating solution into the uterus of the sow to activate the spermatozoa therein.
Claim 5
Dependent Claim
34 words
In the method of impregnating a sow as in claim 1 wherein said boar semen is diluted by said first solution immediately upon collection and said first solution is warmed to approximately 36* C.
Claim 6
Dependent Claim
25 words
In the method of impregnating a sow as in claim 4 wherein said first solution is slowly cooled to approximately room temperature prior to centrifuging.
Claim 7
Dependent Claim
52 words
In the method of impregnating a sow as in claim 1 wherein said resultant sperm solution is maintained at said approximate 5* C from 1 1/2 to 2 hours and is then dropped in droplets upon CO2 dry ice to form solid pellets, said pellets being stored by immersion in liquid nitrogen.
Claim 8
Dependent Claim
31 words
In the method of impregnating a sow as in claim 1 wherein the step of thawing the frozen sperm solution comprises mixing the frozen sperm solution with a heated diluting solution.
Claim 9
Dependent Claim
28 words
In the method of impregnating a sow as in claim 7 wherein said heated diluting solution is of an initial temperature of approximately 60* C to 70* C.
Claim 10
Dependent Claim
81 words
In the method of impregnating a sow as in claim 7 wherein said thawing of said frozen sperm solution is made with an amount of heated diluting solution insufficient to obtain a desired final dilution, and the desired final dilution is obtained by introducing the thawed prediluted solution into the uterus of the sow and thereafter introducing a sufficient amount of said heated diluting solution into the uterus of the sow to obtain the desired final dilution of activated sperm solution.
Claim 11
Dependent Claim
31 words
In the method of impregnating a sow as in claim 7 wherein said heated diluting solution inactivates the spermatozoa at room temperature but activates the spermatozoa at the sow''s body temperature.
Claim 12
Dependent Claim
52 words
In the method of impregnating a sow as in claim 7, characterized in that said solutions used for spermatozoa activation and inactivation contain glucose, sodium citrate, sodium bicarbonate, antibiotics, ethylenediaminetetraacetic acid and potassium chloride, and said freezing diluent contains skimmed milk powder, glucose, egg yolk, glycerine, antibiotics, ethylenediaminetetrAacetic acid and potassium chloride.
Claim 13
Dependent Claim
114 words
In the method of impregnating a sow as in claim 11 wherein said spermatozoa activating and inactiviating solutions contain about 100 to 140 g, preferably about 120 g, glucose, about 10 to 15 g, preferably about 12 g, sodium citrate, about 1 to 3 g, preferably about 2 g, sulfanilamide, about 0.5 to 3 g, preferably about 1.5 g, potassium chloride, about 1.5 to 4 g, preferably about 2.5 g, sodium bicarbonate, about 1.4 to 4 g, preferably about 2.5 g, ethylenediaminetetraacetic acid, about 1.7 millions i. u. penicillin G sodium, about 1.7 i.u. dihydrostreptomycin base in 2,000 ml water, and is adjusted be means of 4% NaOH to a pH of about 6.8.
Claim 14
Dependent Claim
88 words
In the method of impregnating a sow as in claim 11 wherein said freezing diluent contains about 100 to 120 g, preferably about 100 g, skimmed milk powder, about 20 to 30 g, preferably about 25 g, glucose, about 0.5 to 3 g, preferably about 1 g, ethylenediaminetetraacetic acid, about 0.5 to 1.5 g, preferably about 0.8 g, potassium chloride, about 1 million i.u. penicillin G sodium, about 1 million i.u. dihydrostreptomycin base, about 180 ml egg yolk, and about 60 ml glycerine per 1000 ml of water.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1977-02-11 | 1977-08-13 | 1978-02-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1981-02-11 | 1981-08-13 | 1982-02-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1985-02-11 | 1985-08-13 | 1986-02-12 | $7700.00 | Window Closed / Lapsed |