US4945146A
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Dispersion polymers processes for their preparation and their use
Assignee: Siemens Healthcare Diagnostics GmbH Germany
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Filing: 1987-11-18
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Granted: 1990-07-31
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Expires: 2007-11-18
Abstract
Abstract The invention relates to dispersion polymers, processes for their preparation, and their use, and also active dispersion polymers (latex conjugates) prepared therefrom, the dispersion polymers being obtained by polymerizing compounds of the formula I H2C=C(R1)-CO-NH-(CH2)n-CH(OR2)(OR3)I in which n denotes 1-6; R1 denotes H or CH3 and R2 and R3 are identical or different and denote -(CH2)m-CH3, m denotes 0-7, or -CY(X)(Z), in which X, Y and Z denote (CH2)pCH3 and p denotes 1-3, where X, Y and Z are identical or different, and compounds of the formula II H2C=C(R1)-CO-R-(CH2)n-(CHOH)m-(CH2)l-HII in which R denotes O or NH; n denotes 1-3; m denotes 1-4; l denotes 0-4; and R1 denotes H or CH3, with one another in the presence of a seed dispersion, whereby the copolymer is formed on the surface of the latex particles. Latex conjugates are obtained therefrom by bonding the interesting antibodies or antigens to the dispersion polymers according to the invention.
Inventors
- 👤 Wolfgang Kapmeyer
- 👤 Helmut Rinno
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 06:41
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54313; C; C08; C08F; C08F257/00; C08F257/02; C08F291/00
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
187 words
A process for preparing a dispersion polymer comprising the steps of: (a) adding an emulsifier and a free-radical initiator to an aqueous seed dispersion of latex having a particle diameter of 0.02 μm to 2 μm; and (b) adding dropwise with stirring at a temperature in the range of 10° C. to 120° C. to the seed dispersion containing the emulsifier and the initiator a monomer of Formula I ##STR4## in which n denotes 1-6; R 1 denotes H or CH 3 ; R 2 and R 3 are identical or different and denote --(CH 2 ) m --CH 3 , and m denotes 0-7, or ##STR5## in which X, Y and Z denote (CH 2 ) p --CH 3 and p denotes 1-3, where X, Y and Z are identical or different, and a monomer of Formula II ##STR6## in which R denotes 0 or NH; n denotes 1-3; m denotes 1-4; l denotes 0-4; and R 1 denotes H or CH 3 to form a dispersion polymer comprising latex particles and a copolymer of Formula I and II formed on the surface of the particle.
Claim 2
Dependent Claim
187 words
A process for preparing a dispersion polymer comprising the steps of: (a) adding an emulsifier and a free-radical initiator to an aqueous seed dispersion of latex having a particle diameter of 0.02 μm to 2 μm; and (b) adding dropwise with stirring at a temperature in the range of 10° C. to 120° C. to the seed dispersion containing the emulsifier and the initiator a monomer of Formula I ##STR4## in which n denotes 1-6; R 1 denotes H or CH 3 ; R 2 and R 3 are identical or different and denote --(CH 2 ) m --CH 3 , and m denotes 0-7, or ##STR5## in which X, Y and Z denote (CH 2 ) p --CH 3 and p denotes 1-3, where X, Y and Z are identical or different, and a monomer of Formula II ##STR6## in which R denotes 0 or NH; n denotes 1-3; m denotes 1-4; l denotes 0-4; and R 1 denotes H or CH 3 to form a dispersion polymer comprising latex particles and a copolymer of Formula I and II formed on the surface of the particle.
Claim 3
Dependent Claim
20 words
The process of claim 1, wherein mono- or di-hydroxyalkylacrylic or methacrylic compounds are employed as monomers of the formula II.
Claim 4
Dependent Claim
29 words
The process of claim 1, wherein at least one of the compounds N-2,3-dihydroxypropylmethacrylamide, N-2-hydroxypropylmethacrylamide, 2-hydroxypropyl methacrylate, N-2,3-dihydroxypropylacrylamide, N-2-hydroxypropylacrylamide or ethyl 2-hydroxypropylacrylate are employed as monomers of the formula II.
Claim 5
Dependent Claim
28 words
The process of claim 1, wherein acrylamidoalkyl or methacrylamidoalkyl aldehyde di-alkyl acetal wherein alkyl denotes C 2 to C 8 are employed as monomers of the formula I.
Claim 6
Dependent Claim
19 words
The process of claim 1, in which methacrylamido acetaldehyde di-n-pentyl acetal is used as monomers of the Formula I.
Claim 7
Dependent Claim
42 words
The process of claim 1, wherein the mixture of monomers contains, as the compound of the formula II, N-2,3-dihydroxypropylmethacrylamide, N-2-hydroxypropylmethacrylamide, 2-hydroxypropyl methacrylate or the corresponding acrylic compounds, and acrylamidoalkyl or methacrylamidoalkyl acetaldehyde di-n-pentyl acetal is employed as compound of the formula I.
Claim 8
Dependent Claim
27 words
The process of claim 1, wherein the mixture of monomers additionally contains up to 30% by weight, based on the total mixture, of styrene, vinylnapthalene or vinyltoluene.
Claim 9
Dependent Claim
30 words
The process of claim 1, wherein the mixture of monomers additionally contains up to 30% by weight, based on the total mixture, of methacrylic acid, acrylic acid or crotonic acid.
Claim 10
Dependent Claim
57 words
The process of claim 1, wherein the mixture of monomers contains, in addition to monomers of the formulae I and II, compounds from the group comprising styrene, vinylnapthalene or vinyltoluene, and one of the compounds methacrylic acids, acrylic acid or crotonic acid, and in addition up to 20% by weight, based on the total mixture, of dimethylformamide.
Claim 11
Dependent Claim
48 words
The process as claimed in claim 1, wherein the mixture of monomers contains, as compound of the formula II, mono- or dihydroxyalkylacrylic or methacrylic compounds, and acrylamidoalkyl or methacrylamidoalkyl aldehyde dialkyl acetal where alkyl denotes C 2 to C 8 is employed as compound of the formula I.
Claim 12
Dependent Claim
26 words
A biologically active dispersion polymer, comprising a dispersion polymer obtained by the process of claim 1 and an antibody, an antigen or a hapten bonded thereon.
Claim 13
Dependent Claim
42 words
A method of diagnostically detecting an antigen, an antibody or a hapten comprising the step of bonding a dispersion polymer obtained by the process of claim 1 to an antigen, an antibody, or a hapten to form a biologically active dispersion polymer.
Claim 14
Dependent Claim
23 words
The method of claim 12, further comprising the step of detecting the biologically active dispersion polymer by nephelometric, turbidimetric or particle counting methods.
References Cited (Backward Citations)
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