US4940581A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Platelet cryopreservation
Assignee: University of Tennessee Research Foundation
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Filing: 1988-08-15
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Granted: 1990-07-10
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Expires: 2008-08-15
Abstract
Abstract A method for preserving blood platelets by freezing the platelets in contact with a cryoprotectant solution containing a sufficient quantity of prostacyclin for the substantially complete inhibition of platelet function and having a pH which promotes the preservation of the platelets and reconstituting the platelets for infusion.
Inventors
- 👤 James M. Mason
- 👤 David D. Pifer
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 09:31
CPC Classifications
A; A01; A01N; A01N1/00; A01N1/10; A01N1/12; A01N1/122; A01N1/125; A61; A61K; A61K35/00; A61K35/12; A61K35/14; A61K35/19
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
102 words
In a method for the preservation of blood platelets wherein the platelets are frozen in a concentrated state in the presence of a sufficient quantity of prostacyclin for the substantially complete inhibition of platelet function and subsequently thawed in the presence of prostacyclin and at a pH below about 8, the improved step comprising the substantially continuous addition to said thawed concentrate of a sufficient quantity of a diluent to reduce the concentration of the platelets to that quantity acceptable for infusion, and thereafter incubating said diluted platelets at about 37° C. to permit said platelets to substantially recover their pre-freezing morphology.
Claim 2
Dependent Claim
102 words
In a method for the preservation of blood platelets wherein the platelets are frozen in a concentrated state in the presence of a sufficient quantity of prostacyclin for the substantially complete inhibition of platelet function and subsequently thawed in the presence of prostacyclin and at a pH below about 8, the improved step comprising the substantially continuous addition to said thawed concentrate of a sufficient quantity of a diluent to reduce the concentration of the platelets to that quantity acceptable for infusion, and thereafter incubating said diluted platelets at about 37° C. to permit said platelets to substantially recover their pre-freezing morphology.
Claim 3
Dependent Claim
23 words
The method of claim 1 wherein said diluted platelets are incubated for a time period equal to about the half life of prostacyclin.
Claim 4
Dependent Claim
14 words
The method of claim 2 wherein said platelets are incubated for about 15 minutes.
Claim 5
Dependent Claim
19 words
The method of claim 1 wherein said incubation temperature is maintained between about 36.5° C. and about 37.5° C.
Claim 6
Dependent Claim
21 words
The method of claim 1 wherein said substantially continuous addition of diluent continues over a time period of about 5 minutes.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-07-09 | 1994-01-08 | 1994-07-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-07-09 | 1998-01-08 | 1998-07-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-07-09 | 2002-01-08 | 2002-07-10 | $7700.00 | Window Closed / Lapsed |