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US4906749A
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Cyclic anhydride derivatives of chromophors
Assignee: Viomedics Inc
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Filing: 1989-01-24
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Granted: 1990-03-06
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Expires: 2009-01-24
Abstract
Abstract Novel chromophor derivatives of cyclic anhydrides are provided which have the ability to react with a variety of organic substrates forming adducts which are useful in analytical techniques for the detection and measurement of biological compounds.
Inventors
- 👤 Spyros Theodoropulos
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 14:27
CPC Classifications
C; C07; C07D; C07D405/00; C07D405/02; C07D405/12; C07D407/00; C07D407/02; C07D407/12; C07D413/00; C07D413/02; C07D413/12; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/533; Y; Y10; Y10S; Y10S435/00; Y10S435/968
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
172 words
An adduct of an anhydride derivative of chromophors having the structural formula: ##STR7## wherein R is an organic chromophoric group exhibiting fluorescence, luminescence, chemiluminescence or absorption properties; Z is hydrogen, alkyl, aryl, or a heterocyclic group composed of carbon ,hydrogen and at least one of oxygen, nitrogen or sulfur, and wherein Z contains from 1 to 20 carbon atoms; each X contains 2 to 3 straight chain carbon atoms connecting the carbonyl groups; and Y is a divalent or polyvalent group comprised of carbon, hydrogen, and optionally oxygen, and contains up to 20 carbon atoms, and provides at least one single or multivalent alkyl, alkenyl, cycloalkyl, bicycloalkyl, bicyloalkenyl, aryl or carbonyl containing group bridging the carbonyl-containing moieties through one or ore of the carbon atoms of the X groups; L is nitrogen or oxygen; S is an organic substrate; and R' is hydrogen or alkyl, aryl or heterocyclic, of from 1 to 20 carbon atoms; and n is 0 or 1, with the proviso that when L is oxygen n is 0.
Claim 2
Dependent Claim
172 words
An adduct of an anhydride derivative of chromophors having the structural formula: ##STR7## wherein R is an organic chromophoric group exhibiting fluorescence, luminescence, chemiluminescence or absorption properties; Z is hydrogen, alkyl, aryl, or a heterocyclic group composed of carbon ,hydrogen and at least one of oxygen, nitrogen or sulfur, and wherein Z contains from 1 to 20 carbon atoms; each X contains 2 to 3 straight chain carbon atoms connecting the carbonyl groups; and Y is a divalent or polyvalent group comprised of carbon, hydrogen, and optionally oxygen, and contains up to 20 carbon atoms, and provides at least one single or multivalent alkyl, alkenyl, cycloalkyl, bicycloalkyl, bicyloalkenyl, aryl or carbonyl containing group bridging the carbonyl-containing moieties through one or ore of the carbon atoms of the X groups; L is nitrogen or oxygen; S is an organic substrate; and R' is hydrogen or alkyl, aryl or heterocyclic, of from 1 to 20 carbon atoms; and n is 0 or 1, with the proviso that when L is oxygen n is 0.
Claim 3
Dependent Claim
31 words
The adduct of the anhydride derivative of claim 1 wherein R is selected from the group consisting of fluorescein, rhodamine, cresyl violet, Nile blue A, an oxazinne, a thiazine and luminol.
Claim 4
Dependent Claim
24 words
The adduct of claim 1 wherein the anhydride derivative of the chromophor has the structural formula: ##STR8## wherein R and Z are as indicated.
Claim 5
Dependent Claim
24 words
The adduct of claim 1 wherein the anhydride derivative of the chromophor has the structural formula: ##STR9## wherein R and Z are as indicated.
Claim 6
Dependent Claim
24 words
The adduct of claim 1 wherein the anhydride derivative of the chromophor has the structural formula: ##STR10## wherein R and Z are as indicated.
Claim 7
Dependent Claim
24 words
The adduct of claim 1 wherein the anhydride derivative of the chromophor has the structural formula: ##STR11## wherein R and Z are as indicated.
Claim 8
Dependent Claim
24 words
The adduct of claim 1 wherein the anhydride derivative of the chromophor has the structural formula: ##STR12## wherein R and Z are as indicated.
Claim 9
Dependent Claim
24 words
The adduct of claim 1 wherein the anhydride derivative of the chromophor has the structural formula: ##STR13## wherein R and Z are as indicated.
Claim 10
Dependent Claim
24 words
The adduct of claim I wherein the anhydride derivative of the chromophor has the structural formula: ##STR14## wherein R and Z are as indicated.
Claim 11
Dependent Claim
24 words
The adduct of claim 1 wherein the anhydride derivative of the chromophor has the structural formula: ##STR15## wherein R and Z are as indicated.
Claim 12
Dependent Claim
17 words
The adduct of claim 1 wherein the anhydride derivative of the chromophor has the structural formula: ##STR16##
Claim 13
Dependent Claim
17 words
The adduct of claim 1 wherein the anhydride derivative of the chromophor has the structural formula: ##STR17##
Claim 14
Dependent Claim
17 words
The adduct of claim 1 wherein the anhydride derivative of the chromophor has the structural formula: ##STR18##
Claim 15
Dependent Claim
23 words
The adduct of claim 1 wherein said organic substrate is a material which is employed in the detection and measurement of biological compounds.
Claim 16
Dependent Claim
24 words
The adduct of claim 14 wherein said substrate is coupled to said anhydride derivatives of the chromophor through an active site on said substrate.
Claim 17
Dependent Claim
25 words
The adduct of claim 15 wherein said active site is selected from the group consisting of primary amines, secondary amines, hydroxyl groups and mercapto groups.
Claim 18
Dependent Claim
11 words
The adduct of claim 14 wherein said substrate is spherical beads.
Claim 19
Dependent Claim
12 words
The adduct of claim 17 wherein said beads are compose of polystyrene.
Claim 20
Dependent Claim
11 words
The adduct of claim 14 wherein said substrate is a protein.
Claim 21
Dependent Claim
11 words
The adduct of claim 14 wherein said substrate is an antibody.
Claim 22
Dependent Claim
11 words
The adduct of claim 14 wherein said substrate is an antigen.
References Cited (Backward Citations)
US4600775A
Cited
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