US4900665A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Integral multilayer analytical element for use in the measurement of alkaline phosphatase activity
Assignee: Fujifilm Holdings Corp
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Filing: 1988-04-19
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Granted: 1990-02-13
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Expires: 2008-04-19
Abstract
Abstract An integral multilayer analytical element for use in the measurement of alkaline phosphatase activity, which comprises a porous spreading layer of woven fabric or knitted fabric containing an alkaline phosphatase-sensitive self-developing substrate, a buffer layer containing one or more compounds functioning as a phosphoric acid acceptor and a buffering agent and a support layer in a laminated form.
Inventors
- 👤 Kaoru Terashima
- 👤 Shigeki Kageyama
- 👤 Harumi Katsuyama
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 01:06
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/525; G01N33/526; C; C12; C12Q; C12Q1/00; C12Q1/34; C12Q1/42; C12Q2334/00; C12Q2334/10; Y; Y10; Y10S; Y10S435/00; Y10S435/805
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
59 words
An integral multilayer analytical element for use in the measurement of alkaline phosphatase activity, which comprises in sequential order a porous spreading layer of woven fabric or knitted fabric containing an alkaline phosphatase-sensitive self-developing substrate, a buffer layer containing a compound functioning as a phosphoric acid acceptor and a buffering agent and a support layer in a laminated form.
Claim 2
Dependent Claim
59 words
An integral multilayer analytical element for use in the measurement of alkaline phosphatase activity, which comprises in sequential order a porous spreading layer of woven fabric or knitted fabric containing an alkaline phosphatase-sensitive self-developing substrate, a buffer layer containing a compound functioning as a phosphoric acid acceptor and a buffering agent and a support layer in a laminated form.
Claim 3
Dependent Claim
27 words
The integral multilayer analytical element as claimed in claim 1, wherein said phosphoric acid acceptor is an amino-alcohol having a pKa value of not lower than 9.0.
Claim 4
Dependent Claim
33 words
The integral multilayer analytical element as claimed in claim 2, wherein said amino-alcohol is a member selected from the group consisting of 2-(methylamino)ethanol, 2-(dimethylamino)ethanol, 2-(isopropylamino)ethanol, 2-(ethylamino)ethanol, 3-aminopropanol, DL-2-amino-1-propanol, N-methylglucamine, hydroxyproline, 2-aminoethanol and 2-amino-2-methyl-1-propanol.
Claim 5
Dependent Claim
21 words
The integral multilayer analytical element as claimed in claim 1, wherein said self-developing substrate is a self-developing aryl phosphate amine salt.
Claim 6
Dependent Claim
61 words
The integral multilayer analytical element as claimed in claim 4, wherein said self-developing aryl phosphate amine salt contains an aryl group selected from the group consisting of nitro-substituted phenyl, phenolphthalein, thymolphthalein, arylazo-substituted aryl group and fluorescein group and the amine of said salt is selected from the group consisting of tris(hydroxymethyl)aminomethane, 2-amino-2-methyl-1,3-propanediol, 2-amino-2-ethyl-1,3-propanediol, bis(cyclohexyl)amine, 2-(ethylamino)ethanol, N-methylglucamine, 2-(methylamino)ethanol, 2-(dimethylamino)ethanol, 2-(isopropylamino)ethanol and 2-amino-2-methyl-1-propanol.
Claim 7
Dependent Claim
27 words
The integral multilayer analytical element as claimed in claim 4 wherein said phosphoric acid acceptor is an amino-alcohol having a pKa value of not lower than 9.0.
Claim 8
Dependent Claim
33 words
The integral multilayer analytical element as claimed in claim 6 wherein said amino-alcohol is a member selected from the group consisting of 2-(methylamino)ethanol, 2-(dimethylamino)ethanol, 2-(isopropylamino)ethanol, 2-(ethylamino)ethanol, 3-aminopropanol, DL-2-amino-1-propanol, N-methylglucamine, hydroxyproline, 2-aminoethanol and 2-amino-2-methyl-1-propanol.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-02-12 | 1993-08-14 | 1994-02-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-02-12 | 1997-08-14 | 1998-02-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-02-12 | 2001-08-14 | 2002-02-13 | $7700.00 | Window Closed / Lapsed |