US4555484A
Expired / Lapsed
US
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Analytical element and method for alkaline phosphatase assay
Assignee: Clinical Diagnostic Systems Inc
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Filing: 1983-07-25
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Granted: 1985-11-26
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Expires: 2003-07-25
Abstract
Abstract Disclosed herein is a dry analytical element and a method of using same for quantitatively detecting alkaline phosphatase in an aqueous liquid. The element comprises, in fluid contact, first and second reagent zones which can be self-supporting or carried on a support. The first reagent zone contains a substrate for alkaline phosphatase, e.g. p-nitrophenyl phosphate, and the second reagent zone contains a buffer which is an alkali metal or ammonium salt and has a pKa in the range of 9-11.5, e.g. an alkali metal salt of carbonic acid.
Inventors
- 👤 Denise D. LaRossa
- 👤 Allen L. Thunberg
- 👤 Gary E. Norton
- 👤 Glen M. Dappen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 23:18
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/34; C12Q1/42; C12Q2334/00; C12Q2334/10; Y; Y10; Y10S; Y10S435/00; Y10S435/805
Claims Analysis
Total Claims: 25
Claim 1
Independent Claim
74 words
A dry analytical element for the quantitative determination of alkaline phosphatase in an aqueous liquid, said element comprising a support having therein, in fluid contact, first and second reagent zones, said first reagent zone consisting essentially of a substrate for alkaline phosphatase and said second reagent zone consisting essentially of a buffer which is an alkali metal or ammonium salt and has a pKa in the range of from about 9 to about 11.5.
Claim 2
Dependent Claim
74 words
A dry analytical element for the quantitative determination of alkaline phosphatase in an aqueous liquid, said element comprising a support having therein, in fluid contact, first and second reagent zones, said first reagent zone consisting essentially of a substrate for alkaline phosphatase and said second reagent zone consisting essentially of a buffer which is an alkali metal or ammonium salt and has a pKa in the range of from about 9 to about 11.5.
Claim 3
Dependent Claim
17 words
The element of claim 1 wherein said substrate is an organic mono- or diester of phosphoric acid.
Claim 4
Dependent Claim
13 words
The element of claim 2 wherein said phosphoric acid ester is p-nitrophenyl phosphate.
Claim 5
Dependent Claim
9 words
The element of claim 1 comprising a phosphate acceptor.
Claim 6
Dependent Claim
13 words
The element of claim 4 wherein said phosphate acceptor is an amino alcohol.
Claim 7
Dependent Claim
11 words
The element of claim 5 wherein said phosphate acceptor is 2-amino-2-methyl-1-propanol.
Claim 8
Dependent Claim
16 words
The element of claim 1 comprising a subbing zone between said first and second reagent zones.
Claim 9
Dependent Claim
37 words
The element of claim 1 wherein said buffer is present at a coverage of up to about 6 g/m 2 , and said substrate is present at a coverage of up to about 5 g/m 2 .
Claim 10
Dependent Claim
16 words
The element of claim 1 wherein said buffer is an alkali metal salt of carbonic acid.
Claim 11
Dependent Claim
74 words
A dry analytical element for the quantitative determination of alkaline phosphatase in an aqueous liquid, said element comprising a support having thereon, in fluid contact, first and second reagent zones, said first reagent zone consisting essentially of a substrate for alkaline phosphatase and said second reagent zone consisting essentially of a buffer which is an alkali metal or ammonium salt and has a pKa in the range of from about 9 to about 11.5.
Claim 12
Dependent Claim
74 words
A dry analytical element for the quantitative determination of alkaline phosphatase in an aqueous liquid, said element comprising a support having thereon, in fluid contact, first and second reagent zones, said first reagent zone consisting essentially of a substrate for alkaline phosphatase and said second reagent zone consisting essentially of a buffer which is an alkali metal or ammonium salt and has a pKa in the range of from about 9 to about 11.5.
Claim 13
Dependent Claim
17 words
The element of claim 10 wherein said substrate is an organic mono- or diester of phosphoric acid.
Claim 14
Dependent Claim
21 words
The element of claim 10 wherein said buffer has a pKa in the range of from about 9.5 to about 11.
Claim 15
Dependent Claim
14 words
The element of claim 10 comprising a phosphate acceptor in said second reagent zone.
Claim 16
Dependent Claim
56 words
A dry analytical element for the quantitative determination of alkaline phosphatase in a biological fluid, said element comprising a support having thereon, in order and in fluid contact, a first reagent layer consisting essentially of a buffer which is an alkali metal salt of carbonic acid; and a second reagent layer consisting essentially of p-nitrophenyl phosphate.
Claim 17
Dependent Claim
56 words
A dry analytical element for the quantitative determination of alkaline phosphatase in a biological fluid, said element comprising a support having thereon, in order and in fluid contact, a first reagent layer consisting essentially of a buffer which is an alkali metal salt of carbonic acid; and a second reagent layer consisting essentially of p-nitrophenyl phosphate.
Claim 18
Dependent Claim
16 words
The element of claim 14 comprising a subbing layer between said first and second reagent layers.
Claim 19
Dependent Claim
14 words
The element of claim 15 wherein said second reagent layer is a spreading layer.
Claim 20
Dependent Claim
7 words
The element of claim 15 containing 2-amino-2-methyl-1-propanol.
Claim 21
Dependent Claim
59 words
A dry analytical element for the quantitative determination of alkaline phosphatase in a biological fluid, said element comprising a support having thereon, in order and in fluid contact, a first reagent layer consisting essentially of p-nitrophenyl phosphate; a subbing layer; and a second reagent layer consisting essentially of a buffer which is an alkali metal salt of carbonic acid.
Claim 22
Dependent Claim
59 words
A dry analytical element for the quantitative determination of alkaline phosphatase in a biological fluid, said element comprising a support having thereon, in order and in fluid contact, a first reagent layer consisting essentially of p-nitrophenyl phosphate; a subbing layer; and a second reagent layer consisting essentially of a buffer which is an alkali metal salt of carbonic acid.
Claim 23
Dependent Claim
7 words
The element of claim 18 containing 2-amino-2-methyl-1-propanol.
Claim 24
Independent Claim
95 words
A method for the quantification of alkaline phosphatase in an aqueous liquid, said method comprising the steps of: (A) physically contacting a sample of said liquid and an analytical element, said element comprising a support having thereon, in fluid contact, a first reagent layer consisting essentially of a substrate for alkaline phosphates; and a second reagent layer consisting essentially of a buffer which is an alkali metal or ammonium salt and has a pKa in the range of from about 9 to about 11.5, to produce a quantifiable change; and (B) quantitatively detecting said change.
Claim 25
Independent Claim
95 words
A method for the quantification of alkaline phosphatase in an aqueous liquid, said method comprising the steps of: (A) physically contacting a sample of said liquid and an analytical element, said element comprising a support having thereon, in fluid contact, a first reagent layer consisting essentially of a substrate for alkaline phosphates; and a second reagent layer consisting essentially of a buffer which is an alkali metal or ammonium salt and has a pKa in the range of from about 9 to about 11.5, to produce a quantifiable change; and (B) quantitatively detecting said change.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-11-25 | 1989-05-27 | 1989-11-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-11-25 | 1993-05-27 | 1993-11-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-11-25 | 1997-05-27 | 1997-11-26 | $7700.00 | Window Closed / Lapsed |