US4306020A
Expired / Lapsed
US
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Composition for the analysis of the alkaline phosphatase and method therefor
Assignee: Istituto Sieroterapico e Vaccinogeno Toscano Sclavo SpA
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Filing: 1980-09-25
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Granted: 1981-12-15
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Expires: 2000-09-25
Abstract
Abstract A novel composition is disclosed for determining the activity of the alkaline phosphatase, which comprises a salt of p-nitrophenylphosphoric acid, a magnesium salt, sodium borate and 2-amino-2-hydroxymethyl-1,3-propanediol. The composition affords advantages over the known composition especially as regards sensitivity.
Inventors
- 👤 Franco Meiattini
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 17:55
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/34; C12Q1/42; C12Q2334/00; C12Q2334/10
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
25 words
A composition for the analysis of alkaline phosphatase, said composition consisting essentially of a salt of p-nitro-phenylphosphoric acid, a magnesium salt, sodium borate and 2-amino-2-hydroxymethyl-1,3-propanediol.
Claim 2
Dependent Claim
25 words
A composition for the analysis of alkaline phosphatase, said composition consisting essentially of a salt of p-nitro-phenylphosphoric acid, a magnesium salt, sodium borate and 2-amino-2-hydroxymethyl-1,3-propanediol.
Claim 3
Dependent Claim
25 words
A composition for the determination of alkaline phosphatase according to claim 1, wherein the 2-amino-2-hydroxymethyl-1,3-propanediol is present at a concentration between 0.7 molar to 2-molar
Claim 4
Dependent Claim
29 words
A composition for the analysis of alkaline phosphatase according to claim 1 wherein the salt of the p-nitrophenylphosphoric acid is selected from among the salts of 2-amino-2-methyl-1,3-propanediol, and cyclohexylamine.
Claim 5
Dependent Claim
26 words
A composition for the analysis of alkaline phosphatase according to claim 1 wherein the magnesium salt is selected from among magnesium acetate, aspartate, chloride and sulfate.
Claim 6
Independent Claim
53 words
A method for the determination of non-placental alkaline phosphatase which comprises contacting a sample containing both placental and non-placental alkaline phosphatase to be tested with a composition consisting essentially of a salt of p-nitrophenylphosphoric acid, a salt of magnesium, sodium borate and 2-amino-2-hydroxymethyl-1,3-propanediol and thereafter photometrically determining the amount of non-placental alkaline phosphatase.
Claim 7
Independent Claim
53 words
A method for the determination of non-placental alkaline phosphatase which comprises contacting a sample containing both placental and non-placental alkaline phosphatase to be tested with a composition consisting essentially of a salt of p-nitrophenylphosphoric acid, a salt of magnesium, sodium borate and 2-amino-2-hydroxymethyl-1,3-propanediol and thereafter photometrically determining the amount of non-placental alkaline phosphatase.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-12-14 | 1985-06-15 | 1985-12-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-12-14 | 1989-06-15 | 1989-12-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-12-14 | 1993-06-15 | 1993-12-15 | $7700.00 | Window Closed / Lapsed |