Patent US4897468A successfully refreshed from Google Patents.
US4897468A
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Immobilization of peptide-containing compounds on metal hydroxide gels
Assignee: Shimadzu Corp
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Filing: 1988-09-09
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Granted: 1990-01-30
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Expires: 2008-09-09
Abstract
Abstract Peptide-containing compounds such as enzymes, antibodies and antigens are immobilized on metal hydroxy gels formed by hydrolysis of metal alkoxides in an aqueous solvent while evaporating off the solvent. Preferably, hydrogen fluoride is present in the solvent during hydrolysis to provide a fluoride-substituted metal hydroxy gel. Water containing lower alcohol is a preferred solvent. The gel is treated with a coupling agent and a peptide-containing compound is chemically bonded to the gel.
Inventors
- 👤 Shotaro Oka
- 👤 Shu Tahara
- 👤 Hiroyoshi Minakuchi
- 👤 Hajime Karatani
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 15:36
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/551; G01N33/552; C; C03; C03C; C03C11/00; C03C11/002; C12; C12N; C12N11/00; C12N11/02
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
76 words
A process for preparing a bioreactor, comprising the steps of: (a) hydrolyzing a metal alkoxide compound in an aqueous solvent containing from 0.05 to 1.0 mole of hydrogen fluoride per mole of the metal alkoxide, while evaporating off said solvent, to form a gel of the corresponding fluoride-substituted metal hydroxy compound gel, (b) treating said fluoride-substituted gel with a coupling agent, and (c) chemically bonding a peptide-containing compound to the treated gel to form said bioreactor.
Claim 2
Dependent Claim
76 words
A process for preparing a bioreactor, comprising the steps of: (a) hydrolyzing a metal alkoxide compound in an aqueous solvent containing from 0.05 to 1.0 mole of hydrogen fluoride per mole of the metal alkoxide, while evaporating off said solvent, to form a gel of the corresponding fluoride-substituted metal hydroxy compound gel, (b) treating said fluoride-substituted gel with a coupling agent, and (c) chemically bonding a peptide-containing compound to the treated gel to form said bioreactor.
Claim 3
Dependent Claim
34 words
The process of claim 1, wherein the hydrolysis is carried out in step (a) in an aqueous solvent containing a lower alcohol and 0.1-1.0 mole of hydrogen fluoride per mole of the metal alkoxide.
Claim 4
Dependent Claim
41 words
The process of claim 1, wherein the metal alkoxide is Si(OCH 3 ) 4 or Si(OC 2 H 5 ) 4 , and said alkoxide is hydrolyzed at a pH of 1-3 and a temperature between ambient temperature and 100° C.
Claim 5
Dependent Claim
34 words
The process of claim 3, wherein the hydrolysis is carried out in step (a) in an aqueous solvent containing a lower alcohol and 0.1-1.0 mole of hydrogen fluoride per mole of the metal alkoxide.
Claim 6
Dependent Claim
9 words
The bioreactor produced by the process of claim 2.
Claim 7
Dependent Claim
9 words
The biorecator produced by the process of claim 4.
Claim 8
Dependent Claim
88 words
A process for preparing a bioreactor comprising the steps of: (a) forming an aqueous mixture of a metal alkoxide compound with a lower alcohol and 0.1-1.0 mole of hydrogen fluoride per mole of the metal alkoxide; (b) hydrolyzing the mixture by dispersing the resulting metal alkoxide-hydrofluoric acid aqueous solution in a hot liquid medium to form a gel while evaporating of water and lower alcohol; (c) treating said gel with a coupling agent; and (d) immobilizing a peptide-containing compound by chemical bonds to said coupling agent treated gel.
Claim 9
Dependent Claim
88 words
A process for preparing a bioreactor comprising the steps of: (a) forming an aqueous mixture of a metal alkoxide compound with a lower alcohol and 0.1-1.0 mole of hydrogen fluoride per mole of the metal alkoxide; (b) hydrolyzing the mixture by dispersing the resulting metal alkoxide-hydrofluoric acid aqueous solution in a hot liquid medium to form a gel while evaporating of water and lower alcohol; (c) treating said gel with a coupling agent; and (d) immobilizing a peptide-containing compound by chemical bonds to said coupling agent treated gel.
Claim 10
Dependent Claim
18 words
The process of claim 7, in which the temperature of the liquid medium is 50 to 200° C.
Claim 11
Dependent Claim
9 words
The bioreactor produced by the process of claim 7.
Claim 12
Dependent Claim
9 words
The bioreactor produced by the process of claim 8.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-01-29 | 1993-07-31 | 1994-01-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-01-29 | 1997-07-31 | 1998-01-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-01-29 | 2001-07-31 | 2002-01-30 | $7700.00 | Window Closed / Lapsed |